2018年5月，山東省菏澤市某育肥豬場新進(j木話ìn)仔豬突然出現急性發(fā)病死亡，死亡率章志高達30%以上。爲确診病原，分析病原的遺傳演化規律，對(duì)病死豬進信少(jìn)行了病理組織學(xué)診斷和病毒分離鑒定，并對(d機文uì)分離株的E2基因進(jìn)行了序列測定及遺傳變異分畫務析。結果顯示，組織學(xué)病變符合豬瘟的病變特征，與參考毒株核苷酸來雨和氨基酸的同源性分别爲83.4%~97.9%和88.7%~98.7%，與本實驗室從山東分離的流行毒株的核苷酸和氨相跳基酸同源性均高于其他參考毒株。E2基因遺傳進(jìn)化分析表明，該分離株與本實驗室分離的流行毒株遺傳距離較空喝近，同屬于豬瘟病毒2.1d亞型分支。E2基因推導氨基酸突變位點分析表明，分離株的糖基化位點（T806A）與所有參考株均不相同，其具體生物學(xué)意義有待研究。上美工述結果表明，該豬場仔豬的急性發(fā)病死亡是高新由豬瘟病毒感染所緻，緻病毒株屬于豬瘟病毒2.1d亞型。
Comprehensive D舊雜iagnosis of Swine Fever Cases a美什ndPhylogenetic Analysis
on the Isolated Strain
In May，2018，sudden deaths occurred in s厭媽ome new pigletsi那著n a fattening pig farm in Heze City of都年 Shandong Province，and the mortality rate was more th議煙an 30%.In order 司土to find out the pathogen an內東d to analyze its ge廠聽netic evolution status，pathological histolo雜生gical diagnosis andvirus 自店isolation and identification were ca路生rried out. Then th什舞e E2 gene of theisola討議ted strain was sequenced and analyze為個d for genetic variation. According暗算 tothe results，the histological lesio見可ns were consistentwith the pathologi線妹cal features of swine fever，and the homologies of nucleotides an在作d amino acidsbetween 北離the isolate and the refer拍市ence strains were 83.4% to 97.9% a下還nd 88.7% to98.7黃在%，respectively，at the same time，the homologies were higher 能很when compared with thestrains is化章olated from Shandong than other p森暗rovinces. According to thephylo子房genetic analysis of E2 gene，the isolate was clo玩紙se to the strain i討對solated by thelaborator請現y of Shandong Agr光市icultural University，and both of them belonged to t姐電he 2.1d subtype branchof cla科少ssical swine fever virus快聽（CSFV）. Results of amino acid mutatio著師n siteanalysis sho校吃wed that the glycosylatio笑村n site（T806A）of the isolate wa短站s different from all 舊廠the reference strains，and its specific bi個術ological significancestill needed 腦相to be studied. In co一林nclusion，the epidemic was caused by swine feve哥訊r virus and thevirus s這員train belonged to the 2.1d subtype.
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