爲了分析弓形蟲GT1蟲株GRA15（GRA15GT1）蛋白的反應原性，通過(guò)PCR擴增編碼GRA15GT1 52~635氨基酸肽段的基因片段，構建pGEX-6P-1-GRA15GT1載體，轉化BL21菌誘導表達；通過(guò)SDS-PAGE和Western blot方法進(jìn)行表達驗證及反應原性分析。結果顯明內示：SDS-PAGE及以GST标簽抗體爲一抗進(jìn)行Western blot，均有目的條帶，比理論值稍大；業南以豬弓形蟲陽性血清爲一抗的Western blot條帶與GST抗體孵育後(hòu)的條帶大小一緻。上述結果表明，GRA15GT1蛋白具有較好(hǎo)的反應原性，這(zhè)爲下一步分段那光表達GRA15GT1蛋白，研究其在弓形蟲血清學(xué)分劇報型中的應用奠定了基礎。

Expression and Immunoreactivi師對ty Exploration of火離 GRA15Protein

of Toxoplasma gondi訊到i GT1 Strain

In order to analyze the immunoreacti間中vity of GRA15pr制區otein of Toxoplasma gondii GT1 str雜小ain（GRA15GT1），the gene encoding a 584-residue pep妹慢tide（from aa52 to aa635）was amplified by PCR，then the pGEX-6P-1-GRA15GT1 vect術麗or wasconstructed and transformed 但朋into BL21 strain of E. coli. After in答秒ducibleexpression，the purified GRA資大15 protein were obtainedan海務d verified by SDS-PAGE and Wester少綠n blot. Results showed that，the target strip was 路跳slightly larger thanit雪志s estimated size when the G遠服ST-tag mouse monoclonal antib個章ody was used asprimary antibodies，as well as the swine anti-問們T. gondii positive seru行鐵m. The resultsindi生森cated GRA15GT1 protein had 高看good immunoreact睡弟ivity，which would lay a foun子為dation for the ne子那xt stepof GRA15GT1 protein exp通電ression and its applicat區也ion in serological typing ofToxopla文廠sma gondii.

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