I群禽腺病毒的分離鑒定與同源性分析

2019-03-04 14:56:11 矯海婷等 中國(guó)動物檢疫

爲了解雞群中I群禽腺病毒(FAdV-I)的流行現狀,從山東、黑龍江、吉林等地部分大規模養雞場采集病料師地,進(jìn)行病毒分離、PCR鑒定及同源性分析。結果顯示:從匠跳76份病料中分離到10FAdV-I,分離率爲13%10FAdV-I中,1株爲血清4型,6株爲血清8b型,1株爲血清9型,2株爲血清11型。結果表明,山東、黑龍江、吉林等地均存在一定的FAdV-I流行,主要流行血清型已由4型轉變爲8b型,因此需要評估當前的4型滅活苗能(néng)否有效防控這(zhè)些地區房水的FAdV-I流行。


Isolation and Ide會討ntificationand Homology Analysis on Gr城路oup I Fowl Adenovirus

In order to recognize the prevale村腦nce ofgroup I fowl ade答草novirusFAdV-Iin poultrysamples were colle機麗cted from several large訊報-scalechicken farms in Shando制務ngHeilongjiang and Jilin 算影provinces for FAdV isolatio吧說nPCR assay and ho的裡mology analysis. Resu秒書ltsshowed that 10 FA船刀dV-I strains were isolated from高去 76 samples with t懂拿he positiverate reaching 13%. Among 明綠the 10 FAdV-I strains1 of them belonged to serotype 46 of them belonged to serot木美ype 8b1 of them belonged to serotype 9and 2 of them belonged to 鄉理serotype 11. Theresults indi紙去cated that certain FAdV-I i路朋nfection existed白村 in ShandongHeilongjiang and Jilin p頻歌rovincesand the main serotypes had been tra微坐nsferredfrom serotype 4 to唱數 serotype 8bthus the current serotyp站花e 4 inactivated vaccines need to 些我be evaluatedto det女朋ermine if they could地雨 control the spread of FA白她dV-I effectively男山.

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