犬副流感病毒D121004株的分離鑒定及其F基因遺煙他傳進(jìn)化分析

2019-03-04 14:58南照:58 李 超等 中國(guó)動物檢疫

爲了給犬副流感病毒(CPIV)疫苗研制提供支持,無菌采集有呼吸道(dào)症狀病死犬的腦和肺髒樣(大中yàng)品,處理後(hòu)接種(zhǒng)Marc-145細胞,通過(guò)RT-PCR、紅細胞吸附試驗,對(duì)盲傳3代的細胞培養液進(jìn)行鑒定,成體行(chéng)功分離到1CPIV。該病毒能(néng)使Marc-145細胞出現細胞病變,且具有吸附雞紅細胞的能(néng)力,將(ji吃年āng)其命名爲CPIV-D121004株。對(duì)該毒株的F糖蛋白全基因進(jìn)行克隆及核苷酸同源性分析并構建遺傳進(jìn)化樹,亮討發(fā)現分離株的F基因較爲保守,未出現大的遺傳變異。今後(hòu)需要對(duì)分離株的車通免疫原性進(jìn)行研究,以探究其作爲候選疫苗株的潛力。


Isolation and Identificationof Canine P微你arainfluenza Virus D121004 Strain

and Sequence Analysis on Its腦木F Gene

In order to research歌紙 and develop a vaccineagainst canine 東能parainfluenza virusCPIV),the brains and lungs of dead dogs suspi山慢cious of respiratory symptomswer就東e collected asepticallyand then Marc-145民國 cells were inoculated afte鐵視r treatment to isolateCPIV. RT-PCR an弟劇d erythrocyte adsor還樂ption assay were applied to identify現器 the cellculture medium with three b吃南lind passages. The答在 results showed t店開hat one strain ofcanine些廠 parainfluenza virusCPIV-D121004was successfully isolatedwhich could cause cytopathic 師爸changes in Marc-145 cellsand was cap畫拿able of adsorbing chicken erythr子爸ocytes. Cloning and nucleotide homol為什ogyanalysis on the 風購whole F glycoprotein gene were carried 家海outand the genetic evolution tree was 低資built.The results showed that t一煙he F gene of the 山作isolated strain was relativelyc視綠onservativeindicating no major ge都票netic variation西吧. Infuturethe immunogenicit我來y of the isolate shou厭上ld befurther studie從綠dso as to explore the potential to be 問畫thevaccine strain.

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