爲探索适合臨床高通量雞傳染性喉氣管炎檢測的方法，針對(duì)讀做雞傳染性喉氣管炎病毒gB基因序列保守區域，篩選1對(duì)特異性引物信讀和1條特異性探針，建立了實時(shí)熒光PCR檢測方法，男時并對(duì)該方法進(jìn)行特異性、敏感性評估和臨了老床應用檢測。結果顯示：篩選出的引物和探針與其他禽類常見病毒票些無交叉反應，檢測下限達到100拷貝/反應；對(du員金ì)來自13個場的480份臨床樣(yàng)本進家在(jìn)行檢測，檢測出17個陽性樣(yàng)品，與常規拍海PCR檢測結果符合率爲100%。結音森果表明，此方法特異性強，靈敏度高、耗時(shí)少，可通都用于雞傳染性喉氣管炎的快速檢測。

Establishment and A自鐵pplication of a電鐵 Real-time PCR for&男筆nbsp;Detecting Infectious Laryngotr農木acheitis Virus

In order to develop an app懂路ropriate method for業學 high-throughput dete河了ction of avian infectious laryngotrache影商itis（AILT），a real-tim花著e PCR assay was establishe月鄉d with a pair of specific p新商rimers and a probe based on t體熱he conservative zone of用答 gB gene sequence o熱樂f AILT，then its specificit又場y and sensitivit近玩y were evaluated，and its clinical ap水業plication was tested. The resul友農ts showed that the select跳錯ed primers and probe faile制年d to cross-react with oth妹坐er common poultry viruse車說s，with the detection l的輛imit of 100 copies懂鐘/reaction. 17 out of 月說480 clinical samples collected from 13 門自farms were positive. The r信站esults completely conformed to the老還 test result by 對站common PCR assay. As a conclusion，AILT要坐 could be rapidly detected 都站by the established method 紅得with high specificity，high se在訊nsitivity and less time-c個光onsuming.

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