2018年山東省某養殖場發(fā)朋商生大量幼齡鹦鹉死亡事(shì)件，疑似爲病毒感染。爲探亮美尋病因，開(kāi)展了該場及省内另外兩(liǎ如我ng)個鹦鹉養殖場的流行病學(xué)調查。利用PCR技術對(du下湖ì)臨床樣(yàng)品中提取的DNA或RNA進(jìn)行檢測，結老的果發(fā)現新城疫與禽流感病毒均呈陰性，而禽多瘤病毒（APV1）與鹦鹉喙少新羽病病毒（PBFDV）呈現爲強陽性，由女和此推斷此3個鹦鹉養殖場存在APV1和PB技唱FDV感染，平均病毒檢出率分别爲67.9%與71.4%，共感染檢月輛出率率爲58.0%。對(duì)陽性樣(yàng計化)品進(jìn)化全基因分析發(fā)現：區域内的PBFDV流行從謝毒株同源性高，與該地區早期報道(dào)站美的毒株親緣關系較爲接近；APV1的VP遠男1基因同源性較高，與歐洲分離毒株親緣關系較爲接近，說(shuō)明照懂我國(guó)流行的APV1或來源于進(jìn)口鹦鹉。本研究警示，頻朋需要加強鹦鹉疾病防控，嚴格鹦鹉進(日在jìn)出口檢疫，并制定和健全标準的鹦鹉病鐘器檢疫程序。
Pathogen Detection and Sequence Ana兵裡lysis of Aves Polyomavirus 1 and&nbs光朋p;Psittacine Beak & Feather 北子Disease Virus in Three Parrot Fa慢照rms in Shandong Provin嗎門ce
In 2018，a large number 很體of young parrots died in a farm 日山in Shandong Province，which was suspec票我ted of being infected with 藍家virus. In order to find out有姐 relevant causes，an e到月pidemiological inves件線tigation was carried out in the in喝她fected farm and工紙 other two farms i微什n Shandong Provinc裡地e. DNA or RNA extracted from但她 clinical samples 雨錢was tested by PCR，it was foun機區d that Newcastle disease vi唱些rus（NDV）and avian influenza virus（A男答IV）were detected文雪 negative，while Aves司東 polyomavirus 1（APV1）a了白nd psittacine beak & feather disease vi家劇rus（PBFDV）were detected好信 strongly positive，therefore，a concl是子usion was given that parrots in th窗小e three farms had alrea一高dy been infected with APV1 and 金北PBFDV，with average positive rate o我服f 67.9% and 71.4%線鐵 respectively，and 站坐the average co-infec城為tion rate was 5我說8.0%. As per the result要去s of whole-genome analysis on these下車 positive samples，the金藍 prevalent strains 坐麗of PBFDV in the region shared hi花銀gh homology，which was mo林件re similar to those reported earlier；門術the VP1 genes of AP低你V1 were with higher hom現家ology，which were mo匠人re closer to those isolated in Eur購計ope，indicating that the APV1 prevalen資吧t in China might be intr懂錢oduced by impor綠藍ted parrots. Hence，i場相t was necessary to strengthen the區事 prevention of parrot diseases and in短少crease quarantine indicators 業紅related to imported parrots in China.
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