爲了解我國(guó)豬肺炎支原體種(zhǒng)群結構和進(jìn)知媽化關系，2018—2019年采集山東、廣西、河南、江西行制、雲南、天津、湖南等地豬組織樣(yàng)品1 242份，進(jìn)行豬肺分吧炎支原體熒光定量PCR和巢式PCR檢測，并對(duì)測序确認的87風飛份陽性樣(yàng)品，采用adk、rpoB、tpiA 3個管家基因作爲分型參內你考進(jìn)行多位點序列分型（MLST），并對(duì)3個管家基因均擴兵們增成(chéng)功的陽性樣(yàng)品進(jìn)行p146基因分型，算嗎分析我國(guó)豬肺炎支原體種(zhǒng)群結構，并結合習那pubmlst數據庫公布的151個序列類型（ST）進(請物jìn)行eBURST分析。結果又門顯示：有24份陽性樣(yàng)品的3個管家基因全部擴增且測序成(chéng技地)功，共分爲10個ST，均爲國(gu家內ó)内外首次報道(dào)；10個ST分爲3個克隆複合體（C車你C）和4個獨特型，其中ST128占41.7%（10/24），樣(廠山yàng)品來自江蘇、河南和廣西紅門，爲本次檢測的主要流行型。結果表明，我國(guó)豬肺炎支原體分布較廣，具有一高妹定的基因型獨特性。本研究爲我國(guó)豬肺炎支原體的流行病學(xué)數線土據庫研究提供了新資料，有助于掌握國(guó)内生都豬肺炎支原體基因型分布情況，進(jìn)一步些兒完善了豬肺炎支原體流行病學(xu做喝é)數據庫。
Multi-locus Sequence Typing of M你動ycoplasma hyopneum船分oniae in Some Regions of China dur爸通ing 2018 to 201知現9
In order to investigate the 拿筆genetic characteristics and就話 evolutionary rela道我tionship of Mycoplasma hyopnu算中moniae（M. hyopnumoniae）in China，1 24男金2 swine tissues were collecte車紅d during 2018 to 2019 from Shandong，G站計uangxi，Henan，Jiangxi，Yunnan，T低森ianjin and Hunan吧很 for detection of M. hyopnumoniae by 船問real-time PCR and nest煙男ed PCR，and 87 posi靜水tive samples wer秒友e confirmed. DNA 很放was extracted from p土飛ositive samples and classified accord廠算ing to p146 gene and mu話雜lti-locus sequence typi水鐵ng（MLST）with adoption of 3廠看 housekeeping genes including區金 adk，rpoB and tpiA，then the population 我草structure of M. hyopnumoniae in China w微厭as analyzed，and eBURST analy會都sis was carried out in 拿請combination of 151 STs publish歌是ed in pubmlst database. The results 什藍showed that all the three hou學鐘sekeeping genes of 24 pos亮西itive samples were 白兒successfully amplifi黃機ed and sequenced，which were divi民員ded into 10 STs and firstl音呢y reported in the world，involving th西自ree clone complexes（CCs）and fou視為r singletons，in which，ST12子相8 was prevalent and accounted f畫媽or 41.7%（10/24）and the samples were co樂離llected from Ji爸問angsu，Henan and Guangxi. It wa輛計s concluded that M. hyopnumoniae 房通spread widely with uniq書暗ue genotypes to som科爸e extent. Therefore，som煙匠e new data was provided for the stud坐要y on epidemiological database從放 of M. hyopnumoniae，which contribu廠綠ted to the iden們外tification of its dist為友ribution and furt技們her enriched relevant database.
全文下載鏈接：https://kns.cnki.net/KCMS/de理哥tail/detail.aspx?d如黑bcode=CJFQ&dbname=CJFDA坐微UTO&filename=ZGDW20200如線7004&v=MTQ2NDlOSE1xSTl林的GWUlSOGVYMUx1eFl了錢TN0RoMVQzcVRyV00xRnJDVVI3長器cWZZT2RvRnkzZ1ZMektQeXJQZWJHNEg=
