爲了解新疆野生北山羊小反刍獸疫病毒（pes志雪te des petits ruminants virus，PPR鄉房V）的分子特征，根據GenBank公布的PPRV基因組序列設鄉匠計并合成(chéng)引物，采用RT-PCR方法和基因測序技術獲得病毒全基因商能序列，應用分子生物學(xué)分析軟件，對(d小你uì)分離的PPRV毒株進(jìn)行序列分析。訊生結果顯示，本次分離的PPRV毒見務株（China/XJAKS/2017）屬于基因IV型費姐，基因組全長(cháng)15 954 nt，編碼6種(z師資hǒng)結構蛋白和2種(zhǒng)非結構蛋白；在系統進(制美jìn)化上，與國(guó)内新疆分離株China/XJBZ/20明科15、China/XJYL/2013同源率輛從分别高達99.0%、99.6%，與國(guó)外的巴基斯坦了街和塔吉克斯坦分離株親緣關系最近。結果表明，PPRV已經(jīng靜河)在家養動物與野生動物之間傳播。結果提示，PPRV傳說影入野生動物群爲我國(guó)消除PPR帶來極大困難，睡月需加強我國(guó)邊疆地區PPR免疫隔離帶建設，并對(duì)但日野生動物PPRV分子流行病學(xué)特坐影點進(jìn)行追蹤監測。
Molecular Characteri秒一stics of PPRV Isolated from Ibex in 服子Xinjiang
In order to identi下地fy the molecular characteristics of 站近peste des petits ruminants v分裡irus（PPRV）in ibex in Xinjiang，pr說鐘imers were designed and syn資男thesized according to the ge音志nome sequence of PPRV published in Gen但一Bank，the whole gen明木e sequence of the virus was議光 obtained by RT-PCR and gene seq這拍uencing，then the isolated PPRV stra但科in was sequenced and analyzed算又 by molecular biolog科問y analysis software. The r要員esults showed that the isolated PPR們計V strain（China/XJ靜關AKS/2017）fell into genotype IV，wi報自th a total genome length of 15 954 東影nt，encoding 6 s坐化tructural proteins and 2視唱 non-structural proteins；for phylogen東海etic evolution，its大去 homology rates with Xinj船化iang isolates including 她明China/XJBZ/2015 and China/XJYL/201那海3 were 99.0% and鐵爸 99.6%，respectively，and it was clos人南est relative to Pakistan and Tajikistan唱在 isolates. It was conclud作志ed that PPRV had been sprea媽他ding among domestic and 動微wild animals，especiall亮跳y wild animals，which br化可ought difficulties to家化 eradicate it in事家 China，so it was necessary 作師to strengthen the constr吧花uction of isolation zone with vac麗車cination against FMDV in b得答order regions in China，and to t機到rack and monitor the mol錯上ecular epidemiological ch做女aracteristics of PPRV腦船 in wild animals.
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