爲建立快速定量檢測家禽樣(yàng)北金本中禽白血病病毒抗原的方法，對(duì)禽白血病病毒p27蛋白單克隆抗體細司但胞株2E5和3D5進(jìn)行複蘇、鑒場微定，通過(guò)反應條件優化，建立了禽白血病病毒抗睡低原高敏熒光微球快速檢測方法。該方法能(néng)夠快速定量檢測家拿在禽樣(yàng)本中禽白血病病毒，敏感性討我高、特異性強，對(duì)其他家禽病原無交叉反應且具有良好(h金大ǎo)的檢測重複性。對(duì)采自全國(guó)的240份臨床家禽老裡樣(yàng)品進(jìn)行檢測，同時(shí)用美場友國(guó)IDEXX公司生産的ELISA抗原試劑盒開(kāi)展比較呢得，結果發(fā)現兩(liǎng)種(zhǒng)街問方法符合率達93.98%，其中陽性樣(yàng)品符合率爲還吃90.83%，陰性樣(yàng)品麗費符合率爲95.83%；組内與組間變異系到海數分别低于10%和15%。本研究建立的禽白血病病毒p27抗原高敏熒費費光微球快速檢測方法，特異性高、敏感性強，操作簡單、飛數快速，具有較高的應用推廣價值。

Establishment of頻討 the Highly Sensitive a頻愛nd Rapid Fluorescence Q少在uantitative Detection for師哥 Avian Leukemia Virus

In order to establish a ra身照pid and quantitative method for看業 detection of avian leukosis viru業音s（ALV）in poultry samples，2E5 and 器南3D5，the monoclonal antibody cell 煙照strains of ALV p27 protein were re國在vived and identifi公黑ed，a highly sensitive and拍友 rapid fluorescence qua飛裡ntitative detection metho林資d，with high sensiti朋生vity and specificity and go土市od test repeatability and no cros也分s reaction to other pathogens，w樂車as established through optimization of話電 reaction conditions，by which，ALV co技短uld be rapidly and qu來說antitatively detected f物自rom poultry samples. 240 clinic站船al samples collected across China were術風 detected and compared with E算了LISA kit produced理吧 by IDEXX. It was土地 found that the coincidence rate鐘可 of the two methods w女謝as 93.98%，in which the coincidence拍喝 rate of positive sam呢醫ples was 90.83%，and that of negat訊廠ive ones was 95.83%. The coeffic短線ients of variation in i風小ntra group and inter grou服風p were lower th友土an 10% and 15%，respecti都一vely. In conclusion，the established m黑日ethod was worthy of being applied and 麗理expanded as supported by its hig著工h specificity and sensitivity，easy 得看and rapid operation畫店.

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