爲了解湖南豬群豬圓環病毒1型（PCV1樹信）的遺傳變異情況，以PCV1陽性DNA爲模闆，使用冷購PCR方法分兩(liǎng)段擴增P議店CV1全基因序列并進(jìn)行拼接。結些山果共獲得5個PCV1全基因組，其中3株全基因大小爲1759 b但得p，另外2株分别爲1758 bp和1760 bp。5株全基因組同源性爲秒去98.4%~99.7%，與國(guó)内外的PCV1全基因組同源性爲98.1%訊黃~99.7%；ORF2序列同源性爲97.6%~99.6%，與國(gu行路ó)内外的PCV1 ORF2同源性爲96.1%~錢作99.9%。系統發(fā)育樹顯示，5株PCV1均屬于同一分暗長支，并顯示出一定的地理差異，但差異較小。結果表明，湖南省鄉嗎流行的PCV1毒株基因較爲穩定，分離株間差異較小。本研究爲湖南省豬圓環在做病毒病防控及相關研究奠定了基礎。

Whole Genome Sequ亮亮encing of Five Strain錢坐s of PCV1 in Hunan Province and 機司Analysis on Their Gen拿紅etic Evolution

In order to identify the gene開日tic variation of porcine c有上ircovirus type 1（PC劇做V1）in pigs in Huna光妹n Province，taking 服匠PCV1 positive DNA as a template，two sec東房tions of PCV1 gene sequence were am得對plified by PCR and then s懂日pliced together to form the whole gen外工ome. Five strains of whole東雜 genomes were obtai民大ned，in which，three were 1759 木村bp，and two were 1758 bp and 1760 bp雨很，respectively. The homology of t對樹he whole genome w人高as 98.4% to 99.7%，which shared th體鄉e homology of 9畫農8.1% to 99.7% with other 見對whole genomes of PCV1；the hom也關ology of ORF2 sequence was 97.6% to 99.綠對6%，which shared the homology o火關f 96.1% to 99.9% with other PCV1 ORF2.報問 As indicated by the phylogene林體tic tree，all the five strains bel信國onged to the same一知 branch with slightly geogr水朋aphical difference. In conclus訊北ion，the strains were ste頻厭adily prevalent in Hunan Provi黑讀nce with little 做市difference. A foun風路dation was thereby provided for futur月也e prevention and control of P學錢CV in Hunan and for relevant用喝 studies.

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