爲了解山東省豬流行性腹瀉病毒（porcine epidemic diarr妹南hea virus，PEDV）基因呢個組遺傳演化特征，將(jiāng)山東省濱州市某豬場的PEDV陽明不性樣(yàng)品接種(zhǒng)Vero E6細胞，結果業坐成(chéng)功分離到1株PEDV，將(jiāng)東我其命名爲SD20BZ01株。通過(guò)RT-PCR分段擴增病毒土亮基因組，經(jīng)測序、拼接獲得SD20B白明Z01株全基因組序列，并將(jiāng下吃)其與GenBank收錄的參考毒株序列進(jì可自n)行同源性比對(duì)和遺傳進(jìn)訊訊化分析。全基因組序列分析結果顯示，SD20BZ01株與我國(guó)飛見2010年以來流行的GII-b亞群變異株序列同分還源性爲97.8%~98.7%，明顯高于CV777票生、DR13、SM98、SD-M等通光早期經(jīng)典毒株，因此SD20BZ01樹服屬于GII-b亞群變異株。基于S基因的遺傳進(jìn)化年南關系與基于全基因組序列的遺傳進(jìn)化關系花從基本一緻。與CV777株相比，SD20BZ01刀到株S蛋白在中和抗原表位COE處有10個氨基酸突變，會他與近年來變異株S蛋白的氨基酸序列基本一緻，說(shuō)還店明GII-b亞群PEDV的變異規律逐漸趨于穩定。本研究爲山東省PED上書V流行病學(xué)研究及其流行控制策略的制定提供了理論依據。
Isolation，Identification and Genetic睡術 Evolution Analysis知男 of Porcine Epidemic Di對些arrhea Virus SD20BZ01 話和Strain in Shandong Province
In order to investigate the genetic e一飛volution character妹廠istics of porcine epidemic務朋 diarrhea virus（PEDV）in Shandong Prov購村ince，PEDV-positiv朋讀e samples collected from a swine明聽 farm in Binzhou City of Shandong Pro去媽vince were inoculated with 內鄉Vero E6 cells，then a s頻都train of PEDV was succes遠業sfully isolated，then named as 笑見SD20BZ01. Viral genome fragments wer區去e amplified by RT-PCR to obtain its wh花我ole genome sequence throu刀麗gh sequencing and splicin日麗g，then the homology a城志nd genetic evolution relati也計onship were com費說pared with the referen山件ce strain registered in GenBan電遠k. It was found t長要hat，by the analysis of司冷 whole genome sequenc文子e，the homology of SD20BZ0好哥1 and GII-b vari農門ants prevalent in China since 2010 was 月長from 97.8% to 98.7%，which was s子拿ignificantly higher than early clas得車sical strains like CV777，D藍身R13，SM98 and SD-M. It w路鐘as determined that SD20BZ01 was b數都elonged to GII-b s水多ubtype variants. The gene員員tic evolutionary relationship based on 自計S gene was gene長森rally consistent with that based on t兵視he whole genome sequence. Compared水他 with CV777，the S protein of 兵動SD20BZ01 harbored 10 amino ac討新id mutations in the 都湖neutralizing antigen但話 epitope，while was basically consistent間去 with the amino acid sequence of S pro地醫tein of variants prevalent in re輛暗cent years，indicating that the rules of街做 variation of GII-b subtype PEDV te哥放nded to be stable gradually.理劇 A theoretical 路下basis was thus provided for futu煙師re epidemiological research as well as 麗鐘development of prevention and c線我ontrol strategy against 個用PEDV in Shandong Province.
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