禽網狀内皮組織增生症病毒（retic河飛uloendotheliosis virus，REV）是一種(時暗zhǒng)常見的禽腫瘤性病毒，可誘發(fā)感染雞群免疫抑制，給我國照信(guó)養禽業帶來巨大經(j可妹īng)濟損失。本試驗運用DF-1細胞接種(zhǒng)方法，在山東省某微樂父母代白羽肉種(zhǒng)雞場雞群中分離坐暗到1株REV，將(jiāng)其命名爲SD2101株。爲掌握SD210林用1株基因組遺傳演化特征，設計合成(chéng)6對(關開duì)引物，通過(guò)PCR擴增獲得了SD時影2101株的前病毒全基因組序列，并與業醫GenBank下載的參考毒株進(jìn)行了序列比嗎很對(duì)和遺傳進(jìn)化分析。結果顯示：SD2舊著101株前病毒全基因組具有典型複制完整型逆轉錄病毒的基因組結構，與事厭2011年分離自江蘇省的雞源REV野毒株HA1101同源性最高；遺傳唱姐進(jìn)化分析發(fā)現，SD2101株與日什大部分國(guó)内分離株處于同一進(jìn)化支上。爲了解SD到電2101株的緻病性，將(jiāng)分離毒株接種(zhǒng)于1日拿亮齡SPF雞，通過(guò)體質量、免疫器官指日裡數和滅活疫苗免疫應答等指标進(jìn)行評價。結果顯示，SD2101株火筆對(duì)1日齡SPF雞的生長(ch玩做áng)和免疫功能(néng)具有明顯的抑制作用。綜上，與參考毒株相比，技從SD2101株基因組序列變異程度舊在較小，具有一定緻病作用，提示在生産中應加強對(duì)REV的關注和師開監測。本研究爲REV的流行病學(xué)研究提供了信息資拿姐料。
Isolation and I快農dentification of市問 Avian Reticuloendotheliosis Vi你問rus SD2101 Strain from 近理Shandong Province議厭 and Analysis on Its Whole Genom車月e Sequence and Pathogenicity
Reticuloendotheli厭村osis virus（REV），a com黑術mon avian neoplastic virus，could i體錯nduce immunosuppression in i舞舞nfected chickens and bring huge economi大女c losses to poultry indu筆視stry in China. In this study，a空城 REV strain was isolated from嗎開 chicken flocks in a pa通內rental farm in Shandong Prov場金ince through inoculating DF-1 c計拍ells and named as SD2101. In order to 麗機investigate its g樹紅enetic evolution characteristics，樂草six pairs of primers were designed吃作 and synthesized 金匠to obtain the whole genome sequ好新ence of its provirus by司內 using PCR amplification，follo那科wed by sequence alignm吃女ent and analysis on genetic evolutio舞是n with the reference 電輛strains downloaded from GenBank. T照書he results showed that the proviru外舊s was with a typical genome st刀子ructure of replication-competent retrov對來irus，with the highest homology西就 with HA1101 which was isolated fro的小m chickens in Jiangsu Prov算師ince in 2011. It was found that，by anal算站ysis on genetic 費森evolution，SD2101 stra店是in was on the same evolutionar那章y clade as most domestic i說樂solates. In order to id得內entify the pathogenicity of 紅去SD2101 strain，the isolate 近匠was inoculated 家畫into one-day-old specific pathogen 著時free（SPF）chickens，服林which was then eval費好uated by body weight，機農indexes of immune or視制gans and immune response of ina生術ctivated vaccine. In conclus學大ion，the growth a男爸nd immune functions of one-day-ol從了d SPF chickens were significantly inhi妹見bited by SD2101 strain. C照一ompared with the reference strai拍費n，a small degree of genomic sequence va你會riation was observed in SD2為民101 strain，with certain pathogenici知校ty. Scientific 算什data was thus provided f公土or further epidemiologi南明cal research on REV，as it was sugge工亮sted to focus on and mon去低itor the virus in practice.
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