爲實現袋鼠臨床樣(yàng)本中疱疹病毒1型（Macropodid herpesvirus 1，MaHV-1）的出入境快速檢測，基于MaHV-1的gB基因序列設計特異引物和TaqMan探針，建立了一種(zhǒng)MaHV-1熒光PCR檢測方法。試驗結果顯示，該方法隻對(duì)劇空MaHV-1 gB基因呈現特異性擴增，與禽傳染性喉氣管炎病毒、僞狂犬病毒和牛風哥傳染性鼻氣管炎病毒不發(fā)生交叉反應，對(duì)陽性标準質微間粒對(duì)照（pCR-MaHV-1-gB）的最低檢測限爲8個拷貝數/反應。該方法的組内和組間試驗的Ct值變異系數介于0.17%~0.96%之間，具有良好(hǎo)的重現性。試驗村了結果表明，本研究建立的實時(shí)熒空雨光PCR方法可用于袋鼠MaHV-1的病原學(xué)檢測。

Development of a R報大eal-time PCR for Detection ofMacropodi老服d Herpesvirus 1

For rapidly detecting macropodid 長會herpesvirus 1（MaHV-1）in clinic samples from ka匠習ngaroos at entry-exit ports，a real-time PCR was develope拍你d by designingprimers a動森nd probes based 朋計on the sequence of gB gene.業我 The results showed themethod視土 could only amplify the 冷到MaHV-1 gB gene and no cross-rea美你ction with othe家冷rkinds of virus was observed，including infectious來錢 bovine bronchitis 器器virus，avian laryngotracheitis 唱間virus andpseudorabies virus. Th這林e detection limit was 8 copi事空es/reaction for the p從拍ositivestandard plasmid contr視話ol（pCR-MaHV-1-gB）. The coefficients of variation（CV）of intra-assay and inter-assay were b慢分etween 0.17 % to 0.票子96 %，showing good repeatability. In c習請onclusion，the established method was appli吃短cable forpathogen白用ic detection of MaHV-1 f請匠rom the kangaroos.

全文下載鏈接：

http://kns.cnki.net/kcms/detail/deta到這il.aspx?dbcode=CJFD&fi見動lename=ZGDW2019見分01019&dbname=CJFDTE哥影MP