爲滿足禽流感病毒高通量快速檢測的需要，建立了一種(zhǒng)能(né民亮ng)夠檢測各亞型禽流感病毒的實西南時(shí)熒光定量RT-PCR檢測方法，并對(duì)新機該方法的特異性和靈敏度進(jìn)行評估，使用該方法與農業行業标準NY/T77秒遠2—2013中的禽流感病毒RT-PCR方法同時(shí)進(jìn)行臨知的床樣(yàng)品檢測。結果顯示，該方法檢測耗時(shí)短、特相會異性好(hǎo)，檢測下限達10-4 ng/μL，與傳可飛統的RT-PCR方法陽性符合率爲100%。結果表習店明，本方法能(néng)實現對(duì)禽流感病毒的安全、特異音訊、快速、靈敏、簡單、高通量檢測，從而彌補了現有傳統檢測技術的不足不為。

Establishment of Real-time RT-PCR for D民身etection of AvianInfluenza Virus一你

In order to achieve the 飛行high-throughput and rapid detection of國房avian influenza virus（AIV），a real-time fluorescent qu制很antitative RT-PCR method was establish子大edfor detecting all s筆舞ubtypes of AIV. Th到去en its specificity and會人 sensitivity were睡短evaluated，and some c錢不linical samples were tested離木simultaneously by the e和習stablished method and the conventio的術nal RT-PCR stipulatedin the agr這麗icultural industry standard 煙開NY/T772—2013. Results sh鄉會owed that thedet爸照ection limit of the established m商南ethod could reach 10-動關4 ng/μL with shortdetection time and be化都tter specificity，媽山and the positivecoincidence rate笑農 was 100% as compare老土d with traditional RT-PCR method朋員. Inconclusion，the established real-t女短ime RT-PCR method wassafe，specific，r很媽apid，sensitive and simple，and coul話影d achieve thehigh-訊票throughput detection for AIV，which co還些vered thedefici雜道ency of current traditiona東雪l detection technologies.

全文下載鏈接：