2017年12月，上海市一養殖場飼養的豬出現咳嗽、呼吸困難、發(fā)熱及迅速轉歸厭身等病症。將(jiāng)豬鼻拭子接種(zhǒn討看g)雞胚進(jìn)行病毒分離，對(duì)血凝試驗陽飛生性樣(yàng)品在SPF雞胚上進(jìn)一步純化、增殖，分離到1株豬流感資飛病毒（A/swine/Shanghai/1厭地205/2017）。采用RT-PCR對(duì)分離毒株進黑件(jìn)行全基因組擴增測序，利用DNAstar軟件，對(duì刀又)測序基因片段進(jìn)行整個閱機員讀框的核苷酸序列同源性比對(du要年ì)分析，并用MEGA6繪制遺傳進(jìn)化樹并分窗光析氨基酸位點。結果顯示：分離毒株爲H1N1亞型，其8個基答吃因片段均屬于類禽型H1N1進(jìn)化分支，沒(méi)有出家雜現不同基因型流感病毒片段之間的重組；分離株HA蛋白的裂解位點序列爲P微如SIQSR↓G，具有典型低緻病性理用流感病毒的分子特征。本毒株的分離鑒定爲分析我國(guó)大陸地區的豬也雨流感流行狀況和分子特征提供了參考。

Phylogenetic Ana森話lysis on a Strain of Avi作科an-like H1N1 SubtypeSwine報在 Influenza Virus and Determination年鐵 of Its Molecular Cha要訊racteristics

In December 2017，pigs in afarm of Shanghai Cit如用y appeared a series of symptoms，incl冷內udingcough，dyspnea and fever，and th服你ediseased pigs recove靜數red rapidly. Then the nasal swab sample說媽s collected frompigs were inoculated in妹雜to SPF chicken e河媽mbryos for virus isolation，after 城民further purifying and proliferating th來道e positive samplesdetected by 呢暗haemagglutination ass地民ay（HA），one strain of謝喝 swine influenza virus（A/swine/Shanghai姐南/1205/2017）was isolated. The whole gen但了ome of the isolate was ampli事光fied andsequenced by RT-PCR，the遠你 nucleotide sequence homology ofthe wh跳喝ole reading fram雨睡e was analyzed by the D東你NAstar software，and then the g從白enetic evolution tree was des謝睡igned using MEGA 6 toanaly作還ze the amino acid sites. The result國一s showed that the isolated strain 自弟wasidentified as H1N1 subtype一對 influenza virus，and itseight gene fra鄉冷gments were classified into evolu他秒tionary branch of avian-l火些ikeH1N1，with no any r謝歌ecombination among 的水influenza virusfragme身黑nts of various genotypes. T術花he cleavage site sequ長書ence of its HA 多水proteinwas PSIQSR↓G，ind議土icating thetypical molecular charact呢草eristics of low pathogenici商金ty influenza virus.Therefore，the isol東科ation and identification o煙那f H1N1strain would西兒 provide some reference for analyzing t劇地he epidemic situation andmolecular 們就characteristics of月服 swine influenza i會業n Mainland China.