針對(duì)淋巴細胞脈絡叢腦膜炎病都笑毒（lymphocytic choriomeningitis virus些費，LCMV）基因保守序列，設計特異性引物和熒光你跳探針，建立了LCMV實時(shí)妹妹重組聚合酶等溫擴增（real-time r公黃ecombinasepolymerase a拿從mplification，real-time RPA）檢測方法。該檢懂熱測方法具有良好(hǎo)的特異性，與LCMV同步檢測的其他鼠病毒均未出鐵書現交叉反應；該檢測方法具有與RT-PCR一樣(yà人可ng)的高敏感性；通過(guò)對(duì)L錯知CMV感染鼠組織樣(yàng)本和LCMV陰性鼠低河血清樣(yàng)本的檢測，證實建立的實時(sh問市í)熒光RPA方法具有良好(hǎo)的穩定性和可靠性。與現有的核酸檢測兒草方法相比，該實時(shí)熒光RPA檢測方法在核酸上樣(yàng)20 m低要in内即可判讀結果，可滿足齧齒類動票劇物LCMV快速檢疫需要。

Establishment of a Real-tim自窗e RPA Method for Rapi不樂d Detection ofLymphocytic 爸嗎Choriomeningitis Virus

In this paper，specificprimers and fluorescent pro錢工bes were designed a子林ccording to the conse很金rvativesequence of lymphocytic cho道妹riomeningitis virus（LCM能見V），and a real-time recombinase polymera都報se amplification assa話都y（real-time RPA）was established.麗村 Resultsshowed that the speci日見ficity of the ass服器ay was good，andno any cross react動大ion with other murine viruses dete白森cted by LCMV was sh花懂own. Thesensitivity of establ電化ished assay was as high as the RT-PCR m站藍ethod. Otherwise，based on d冷公etection of LCMV infec劇老ted murine tiss外靜ues and negativeserums，the real-醫店time RPA showed good stability andre來科liability. Compared with current dete煙船ction methods of nuc志志leic acids，the test results co綠男uld be interpreted wi工醫thin 20 minutes af見短ter loadingthe nuc黑男leic buffer using the r友房eal-time RPA，which couldsatisfy the n哥學eed of rapid LCMV inspection in rode河離nt animals.