近幾年，在雞群中出現一種(zhǒn綠生g)以肝破裂血水爲主要特征的疾病，簡稱爲肝破裂血水志麗綜合征，給養殖單位造成(chéng)較大經(jīng)濟損失。爲探究雞肝線舞破裂血水綜合征發(fā)生原因，采喝男集11省區市9種(zhǒng)森樹品系雞的458份臨床肝破裂血水樣都月(yàng)本，開(kāi)展病原分就你離以及分子生物學(xué)檢測、病理學(xué)診斷、内毒素檢測和火鐵動物試驗，并結合流行病學(xué)調查進(jìn)行病因探讨。腦現病原分離結果顯示：檢測到大腸杆菌14份，占比2.8%；沙門氏菌水玩7份，占比1.5%；未檢測到彎曲杆菌以及未分離到相關病毒。PCR、R明去T-PCR結果顯示：檢測到禽腺病毒她看（FAdV）3份，占比0.66%；禽戊型肝炎病毒（HEV）1份，占比0.2服吧2%；未檢測到雞傳染性貧血病毒（CIAV）門窗、馬立克病毒（MDV）、禽白血病病毒（ALV）。Ran工就dom PCR檢測未發(fā)現RNA和DNA病毒；内毒素檢司光測發(fā)現15份樣(yàng)本超标些市，占比3.3%。動物試驗結果顯示，腹腔高化攻毒不能(néng)複制出臨床肝破裂視懂血水表征。選取11省區市99份有代還輛表性的肝髒樣(yàng)本，固定後(hòu)病信得理學(xué)診斷發(fā)現：無疑似髓樣(yàng)白血病/白血病病理變化；她務疑似細菌造成(chéng)的壞死大雨性肝炎4份，占比4.04%；疑似禽網狀内皮細胞增生病并發(fā)行紙血管瘤病理變化1份，占比2.02%的友；澱粉樣(yàng)變94份，占比93.94%。流行病學舞答(xué)調查發(fā)現：該病在全國(guó)各地均有發(f志雪ā)病，發(fā)病雞品種(zhǒng)和日齡廣泛，拍一無明顯季節性；同一雞場不同品種(zhǒng)，在飼料、免疫和管理都(dōu樂化)一緻的情況下，有的品種(zhǒng)肝破裂血水，有的品種(zh不廠ǒng)正常。以上研究表明，近幾年雞群中出現的肝破裂血水綜合征病例與服志常見的可引起(qǐ)雞肝破裂出血的因素無明顯相關性窗書，初步分析與雞體本身存在遺傳自身們內免疫性缺陷因素有關，機體反應過(guò)度，出現美日細胞因子風暴綜合征導緻雞肝髒澱粉樣(yàng)變出血，從而出現木自肝髒破裂血水的臨床症狀。
Exploration on th錯快e Causes of Chicken Liver Hemo友車rrhagic Syndrome
In recent years，an animal diseas商到e mainly characterized by liver h好長emorrhage（liver hemorrhagic synd妹生rome）appeared in chicken，resulting in 也場huge economic loss to po坐見ultry industry. In order to identify r關木elevant causes，458 clinical lesio頻微n samples were collected fr還歌om 9 chicken lines in 11 provinces/dis音老tricts/cities for pathogen 服又isolation，molecular bio讀放logical detection，pathol水制ogical diagnosi科跳s，endotoxin test and exper國弟iment on animals，t你門he causes for the disease we靜他re discussed based on epi不對demiological inve商明stigation results. 相就It was shown that公開，by pathogen isolation，14 現很samples of Escherichi老討a coli and 7 sampl愛技es of Salmonella were detected從厭 out，accounting for 玩家2.8% and 1.5%，respective視跳ly，no Campylobacter o森腦r other virus was dete章對cted or isolated. It 務女was concluded that，by 線銀PCR and RT-PCR，3 samples 從讀of fowl adenovirus（fadv）and one白媽 sample of avian h得弟epatitis E virus（HEV去近）were detected out，accounting 哥志for 0.66% and 0.22%，respectively但銀，no chicken infectious anemia virus關動（CIAV），Marek's dise讀什ase virus（MDV）and 謝也avian leukosis virus（ALV）were detecte坐市d. No RNA or DNA virus was found by 飛個Random PCR. 15 samples excee西愛ded the standard for e媽聽ndotoxin，accountin問你g for 3.3%. It was found 紙報that，by experiment on animals，the s匠通ymptoms of clinical liver hemorrha冷數ge could not be reprodu男朋ced by intraperitonea照雜l challenge. 99 representa妹服tive liver samples were 問的selected from 11 provinces/身快districts/cities，followed by being f章亮ixed for patholo什時gical diagnosis，no suspected myeloi火們d leukemia/leukemia path村高ological changes were fo她門und，4 cases of necrotic hepatitis ca又請used by suspect民水ed bacteria，one suspect鐵學ed avian reticuloendotheliosis compli讀自cated with hemangioma and 9和做4 cases of amyloidosis were o答見bserved，accounting for 4白樂.04%，2.02% and 93.94%，respective好鄉ly. By epidemiological investigation，it那高 was found that the disease occurred a地也ll over the country，co章習vering a wide range o美厭f varieties and age，withou南電t obvious seasonality們個；for different varieties議美 in the same farm，some 視場were with liver hemorrhage 高坐but others were no子說rmal under consistent conditions of fe厭視ed，vaccination and m這子anagement. In short，the case人如s of liver hemo就公rrhagic syndrome found in recent y鄉鄉ears were not related to the common 一請factors leading to liver hemorrhagi又年c，which was connected with the gene說看tic autoimmune defects in chick看也en as preliminarily analyzed，specifi熱月cally，the organism煙紙 reacted overly，followed by cytokine 術身storm，resulting in amyloidosis ble務錢eding in chicken liver，and thereby c喝有linical symptoms of live什拿r hemorrhage.
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