爲了解新疆和田地區鵝源沙門氏菌流行新公血清型及其耐藥性，無菌采集和田地區兩(關鐘liǎng)個鵝場（A場和B場）病死鵝内髒、肛拭子樣(yàng)本，用常規雜討方法進(jìn)行沙門氏菌分離鑒定，利用P工拿CR進(jìn)行驗證和血清型鑒定，用K-B法黑風進(jìn)行分離株耐藥性檢測。結果顯示：共分歌街離出14株沙門氏菌，總分離率爲1.81%（不樹14/773）。A場死亡鵝内髒樣(yàn兒放g)本的沙門氏菌分離率爲1.33%（4/300），草見肛拭子樣(yàng)本分離率爲很就1.66%（5/300），在存活病鵝肛拭子中未分離出沙門影還氏菌（0/25）；B場死亡鵝内髒樣(y秒錯àng)本的沙門氏菌分離率爲20.0拿美0%（4/20），肛拭子樣(y新快àng)本中未分離到沙門氏菌（0/19），存活病鵝肛拭子樣(yàng)本分離影藍率爲0.91%（1/109）。共分離出南好4種(zhǒng)血清型，占比分别爲鼠傷寒沙門氏街資菌42.86%（6/14）、腸炎沙門氏菌21.43%（3/14）、德比沙門商筆氏菌7.14%（1/14）、紐蘭沙門氏菌7.14%（1/14）低事。沙門氏菌分離株對(duì)多黏菌素B、哌拉西林-他唑巴坦、阿莫西林-棒酸和公分頭孢吡肟較爲敏感，對(duì)其餘10種(zhǒ地事ng)抗菌藥物具有一定耐藥性，其中對(duì)氨苄西林和鏈黴素和件耐藥性極強。結果表明，和田地區鵝源沙門氏菌血清型複雜，其中鼠傷寒沙學下門氏菌爲優勢血清型，不同養殖場流行的血清型種(zhǒng)類及優勢血清型有下跳差異；流行的鵝源沙門氏菌耐藥較爲普遍務個，且表現爲多重耐藥。結果提示，各養殖場應根據流行的具體血清型沙門氏鐵畫菌株，科學(xué)合理使用臨床抗菌藥物西內。
Serotype Identification and Dru站空g Resistance Analysis of Salmo紅個nella of Goose Origin in Ho靜輛tan，Xinjiang
In order to identify the prevale紅街nt serotypes and drug 問問resistance of Salmonella吃票 of goose origin in Ho妹能tan，Xinjiang，visceral and河哥 anal swabs of dea些窗d geese and anal swabs of sic近黑k geese were aseptically col數票lected from two farm聽這s（Farm A and Farm 城草B）in Hotan for isolation and id冷數entification of 理森Salmonella by conventional me南腦thods，followed 數鐵by validation and serotype identif訊山ication by PCR，then舊低 drug resistance analysis of the is理藍olates by Kirby-Bauer disk di著答ffusion susceptibility tes學商t（KB test）. The resu在筆lts showed that 14 文愛strains of Salmonella were 近車isolated，with a to商雪tal isolation rate of 1.離黃81%（14/773）. For Farm A，th林司e isolation rate of viscer光行al samples of de鐘金ad geese was 1.33%（討師4/300），and that of anal 員美swab samples was 1.66%（5/快如300），but no Sal時人monella was isolated from a短在nal swabs of sick gees她秒e（0/25）. For Farm B，the is著我olation rate of Salmonella from 器爸visceral samples of dead geese was 20議拿.00%（4/20），none was isolated 愛如from anal swab sampl雪紙es（0/19），and that of an作廠al swab samples of sick geese 影林was 0.91%（1/109）. Four se湖個rotypes were isolated，including Sa身現lmonella typhimurium a吃花ccounting for 42.86%要農（6/14），Salmonella enteritis for藍報 21.43%（3/14），Salmonella 內電derby for 7.14%（1/14）and能不 Salmonella new房快land for 7.14%（1/14）. The isolates were哥校 sensitive to polymyxin 些兒B，piperacillin taz件謝obactam，amoxicillin clavulanic acid an制數d cefepime，and resistant t場著o other 10 antibiotics to some ext快笑ent，especially extremely resistant事動 to ampicillin and streptomycin. In co亮麗nclusion，the ser村慢otypes of Salmone問金lla in Hotan were complex，in 少到which Salmonella ty光樂phimurium was t窗喝he dominant one，and the prevalent and影南 dominant serotypes w會亮ere different depending on diff城妹erent farms；the drug resistance，esp來電ecially multiple one，of the preval問你ent Salmonella strains，was common著西. It was therefore recommended討外 that antibiotics shou中人ld be scientifically and rea生姐sonably used in clinical 聽些practice according to spec國家ific serotypes prevalent i到舞n each farm.
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