禽多殺性巴氏杆菌可引起(qǐ)禽出血性敗血船上症，又稱爲禽巴氏杆菌病、禽霍亂，發(fā裡他)病快，死亡率高。爲對(duì)禽多殺性巴氏杆科影菌的緻病機制研究及臨床防治提供站來支持，通過(guò)PCR鑒定、耐藥性分析、生物被(bèi有們)膜形成(chéng)能(néng)力試驗以及半數緻死科樂量（LD50）測定，對(duì)4株臨床分離的雞源多殺性巴氏杆道飛菌（Pm01、Pm03、1801及1803）進(又人jìn)行了相關生物學(xué)特性分析。PCR鑒歌得定結果顯示，4株多殺性巴氏杆菌均爲莢膜血清A國醫型，均含有pfhA、exBDtonB、nanB、oma87、ompH、空黑hgbB、hgbA、sodC、s器下odA 9種(zhǒng)毒力基因，不含有toxA、看吧nanH、ptfA 3種(zhǒng)毒力基因；耐藥性分析結果顯示到拿，4株多殺性巴氏杆菌對(duì)克林黴素、紅黴素制好、四環素、卡那黴素、阿莫西林、頭讀會孢噻吩和氨苄西林等7種(zhǒng)抗生素表現出全部或部分耐藥友雜；生物被(bèi)膜形成(chéng)能(n時工éng)力試驗結果顯示，Pm01菌道多株能(néng)形成(chéng)極強的生物被(bèi)膜，而Pm03、180高個1以及1803菌株幾乎不能(néng)形成(ché資懂ng)生物被(bèi)膜；LD50測定結果顯示，4菌株LD50分别爲1.33×線年101、2.74×102、4.22和4.22 CFU。結果表明，4株雞源嗎見多殺性巴氏杆菌含有多種(zhǒng)毒力基因鐘暗，均具有較強的緻病性，其毒力與生物被(bèi)膜形成(ché錯愛ng)能(néng)力不完全相關，且對(du愛照ì)多種(zhǒng)抗生素耐藥。本研究爲禽巴氏杆菌病防治提供時唱了技術支撐與參考數據。
Analysis on the Biological Char姐著acteristics of Four Strains我廠 of Pasteurella multocida Derived from綠商 Chicken
Avian hemorrhagic septicemia，also k小水nown as avian pasteurellosis or avia近山n cholera，is caused by Pasteurella 聽些multocida，with rapid o鐘是ccurrence and hi月化gh mortality. In order to 工購support future researches on the path關司ogenic mechanism of the b短物acteria as well as prev小吃ention and control of the diseas線錯e in practice，relevant biolog銀大ical characteristics of four學如 strains of Pasteurella multo議間cida（Pm01，Pm03，1801 and 1803）w物身ere analyzed throug舊煙h PCR identification，dr裡兵ug resistance analysis，biofilm for風行mation ability test街看 and median lethal 商時dose（LD50）determi秒票nation. It was showed that銀章，by PCR identificatio很大n，all the four strains wer嗎錯e capsular serotype A 東姐and contained 9 v鐵慢irulence genes including pfhA，exBDtonB微小，nanB，oma87，ompH鐘開，hgbB，hgbA，sodC and sodA，except toxA，na錯人nH and ptfA；by drug resistance an山你alysis，the four strain師公s were fully or那可 partially resistant業用 to 7 antibiotics incl刀答uding clindamycin，ery地制thromycin，tetrac南車ycline，kanamycin，amoxicilli月師n，cefotaxime and 舞議ampicillin；by biofi子南lm formation ability test，a very st拿鄉rong biofilm was formed by Pm01美樹 strain，but failed by Pm03煙行，1801 and 1803；and玩時 by the LD50 det弟要ermination test，the LD50 劇時values of the four str房也ains were 1.33×101，2.74×102，4錯請.22 and 4.22 CFU，respectiv習他ely. In conclusio校音n，all the four strains containe低呢d a variety of vir熱靜ulence genes that were with strong pa金生thogenicity，for which the vir分很ulence was not completely related to答下 the biofilm formation abilit呢現y，and resistant to a件可 variety of antibiotics. A technic低笑al support and referenc習冷e data were provided for future pr笑她evention and co書草ntrol of avian p男商asteurellosis.
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