爲探究豬繁殖與呼吸綜合征病毒（PRRS技制V）在規模豬場的流行特征，2021年從湖北省7個規模豬場疑似PRRSV感染錯短豬群以及所有種(zhǒng)公豬群路劇中，共收集樣(yàng)品3 324份，采用三重聽好巢式RT-PCR法進(jìn)行PRRSV病原學(xué)檢測討到，分析不同養殖類型、不同生長(cháng)階段、不同季節及不同感染毒株的流行喝明特點，并將(jiāng)陽性産物進(jìn)行測序和遺傳進(j飛離ìn)化分析。結果顯示：3 324份樣(yàng)品中志森共檢測出PRRSV陽性樣(yàng)品323份，陽性檢出率爲9愛船.72%（323/3 324）。育種(體東zhǒng)場陽性檢出率最低，爲2.66%（20/相雪752）；商品豬擴繁場與育肥場陽性檢出率相近，分别爲11.河小82%（169/1 430）與11.73%（134/1 142）；保育豬群廠光陽性檢出率最高，爲17.23%（1得作52/882）；其餘依次爲生長(cháng)育肥豬、哺乳仔豬和母豬，陽性檢船公出率分别爲11.30%（121/1 07白年1）、5.26%（30/570）和3.呢多24%（20/618）；種(zhǒng)公豬中未發(fā從這)現PRRSV感染。PRRSV一年四季均可流行，春、會靜夏、秋、冬季的陽性檢出率分别爲18.16%（148/815）我錯、6.94%（60/864）、6.10%（34/557）、7.44%（81村問/1 088），春季陽性檢出率顯著高于其他季節（P＜0.05），而夏場雜、秋、冬季節間差異不顯著（P≥0.05）。3種(zhǒng月麗)PRRSV毒株均有流行，類NADC30毒株、經(jīng)典毒志歌株和高緻病性變異毒株的占比分别爲49.54%、21.36%、29.亮窗10%，類NADC30毒株檢出占比顯著高于其他類型毒株（P可用＜0.05）。結果表明：PRRSV在所調查的7個規模豬場中流年子行普遍，尤其是商品豬擴繁場與育肥場；保育豬群PRRS坐放V感染風險較高，種(zhǒng低通)公豬感染風險較低，春季是高發(f雨志ā)季節，類NADC30毒株爲優勢流行毒株。結果提示，規模我畫養殖場應根據PRRSV的流行特征，加強免疫和監測，有針對(duì)性地制些筆定防控措施。
Epidemiological I報火nvestigation on PRRS in Some I刀到ntensive Swine Farms in Hub頻紙ei Province
In order to investigate 醫刀the epidemiologic妹作al characteristics of porcine repr到器oductive and respiratory syndrome vir器好us（PRRSV）in inte服內nsive swine farms，a total of 3 324 samp門醫les were collected in 2021 from pi在到gs suspected to be infec草費ted with PRRSV and all boar一船s in 7 intensive farms in Hubei Pro銀裡vince for pathogenic detect身白ion by triple-ne從北sted RT-PCR to anal草制yze the prevalent si從廠tuation in different breeding typ這睡es，growth stages，seasons刀道 and strains，follo大什wed by sequencing and ge靜兵netic evolution of positive products.吧信 The results show了商ed that 323 posi場公tive samples were detected，with the 如著positive rate of 9.72%（323/3 324）；愛的the lowest positive detecti哥亮on rate was 2.66%亮煙（20/752）in breeding farms，similar in c看刀ommercial propagation farms醫務 and fattening f技線arms，which were 11.8要河2%（169/1 430）an信吃d 11.73%（134/1 142）respectively；hi友生ghest in nursery pigs（17.23%），f煙北ollowed by growing 道街finishing pigs，suckli來刀ng piglets and 街影sows，with 11.30%（121/1 071），5.26%（3玩雜0/570）and 3.24%（20/618）respectively草師，no PRRSV infection w來女as found in breedi制外ng boars；PRRSV could be preva匠問lent throughout a year海房，and the positive detection rates in s黃計pring，summer，autumn拍日 and winter were 18國很.16%（148/815），6.94%（6下美0/864），6.10%（34/557）and鐘子 7.44%（81/1 088）r商地espectively；that in spring was 相現significantly higher than tho門民se in other seasons（P＜0.05），but with 那影slightly difference in summer，au科物tumn and winter（P≥0.05）；a場動ll three types o長草f strains including NADC3看放0-like strain，classical strain 劇小and highly pathogenic variant s中身train were prevalent，accounting for 49飛作.54%，21.36% and 29.10你外%，respectively，the propo匠綠rtion of NADC30-like strain wa身信s significantly hig外廠her than other types o腦也f strains（P＜0.05）. In concl短外usion，PRRSV was widely prevalent為不 in the 7 farms，件關especially in co報離mmercial propag從男ation farms and fa暗家ttening farms；the女厭 risk of PRRSV infection was high in n鐘跳ursery pigs，lower in b內計reeding boars；and NADC30 like stra文是in was the dominant epidemic strain，商要especially in sp也校ring. Therefore，i我輛mmunization and 新服monitoring should be streng他技thened by the farms according to the pr了女evalent characteristics of PRRSV紙土，and targeted measure新資s for prevention and control should be 慢分developed.
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