爲全面(miàn)掌握雲南省玉溪市小反刍獸疫(P你子PR)的病原分布和羊群免疫效果,根據2021年玉溪市PPR專項監測方家林案,在全市開(kāi)展PPR中年監測和流行病學(xué)調查。采用多階段調查的方快黃法,從全市9個縣區采集免疫羊血清樣(yàng)品1 631份、眼鼻棉少是拭子樣(yàng)品6 438份,分别使用阻斷ELI了子SA和實時(shí)熒光RT-PCR檢測方法低要,進(jìn)行PPR免疫抗體檢測和病毒核酸檢測。結果顯示:2會頻021年玉溪市PPR群體免疫合格率爲82.98%,個體免煙鐘疫合格率爲83.62%,未檢測到病原學(xué)志飛陽性樣(yàng)品;不同養殖區域、不同性别的免疫抗師西體合格率均有統計學(xué)差異兵白(P<0.05),而不同養殖類型、不同企業疫筆理苗的免疫抗體合格率差異均不顯著(P>0.05拍短)。結果表明:玉溪市羊PPR整體免疫效果較好(hǎo麗樂),所用PPR疫苗免疫原性較好(hǎo),免疫程序合理,和唱但部分縣區的免疫合格率較低,因此應繼續加強P數影PR的強制免疫及補免工作;在未引入新媽河疫源的前提下,疫情發(fā)生風險較低,但應加大羊隻引種(海購zhǒng)檢疫和調運監管力度。本次監測評估了玉溪市的PP鄉們R總體防控狀況,爲盡快實現消滅PPR的目标提供了數據支撐開爸。
Special Monitoring and Evaluation on 務能PPR in Yuxi City of Yunnan Provi去時nce in 2021
In order to investigate the di女爸stribution of pathogen of他小 peste des petits 都鐘ruminants(PPR)and immune 雜有effect in sheep in Yuxi City of 會他Yunnan Province,targeted monitoring那民 and epidemiological線花 investigation were carried out ac知購ross the city according to the spec都哥ial monitoring plan for PPR pub視這lished in the c美從ity in 2021. A tota明火l of 1 631 serum samples and他文 6 438 eye or nose swabs wer靜黑e collected from im又頻munized sheep in 9 counties/通場districts in the city by using人北 multi-stage investi小厭gation to respectively det都如ect immune antibodie來農s against PPR and virus n腦拿ucleic acid by blocking ELISA and real-森林time fluorescent RT嗎笑-PCR. The results showed that 土謝the qualified rates o件理f population and individual animal were樂場 82.98% and 83.62%,resp她麗ectively,and no positive samples wer志車e detected;the qu費數alified rates of immune antibodies很街 in different breeding數畫 regions or different genders were業訊 of significant stat相話istical difference(P<0.01),but slight術喝ly in different breeding types an暗一d vaccines produced by different e場嗎nterprises(P>0.05). In conclu農為sion,the overall immunizat地體ion effect against PPR was good in th麗票e city,with good immunogenic道了ity of PPR vaccines 熱分as well as reasonable immuni水車zation procedure,but the q年門ualified rate of immunity好少 was lower in some co拍兵unties/districts,which should conti月紙nue to implement compulsory or supp跳東lementary immunization against PPR謝外;and sheep to be introdu多拍ced should be strictly qu但離arantined and supervised during 技司transportation,al匠從though the risk of outbreak was lower 下見in the context of a司男bsence of any i學畫nfection source. The general status 鄉錢of prevention and contr章呢ol of PPR in the city wa海來s monitored and evaluated in the 錢西paper,from which a reference一那 was provided for reaching 長輛the goal of eliminating PPR soonest.
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