爲了解我國(guó)豬繁殖與呼吸綜合征病毒（porcine reprod理答uctive and respirator讀森y syndrome virus，PRRS去愛V）的流行及遺傳變異情況，2020年對(duì)來自7個省的570份疑到見似PRRSV感染樣(yàng)品進(jìn)行RT-PCR檢測媽和，并對(duì)60個PRRSV陽性樣(yàng雨術)品進(jìn)行ORF5基因測序及分析比較。RT-PCR檢測結果顯示，2開間020年送檢樣(yàng)品中，PRRSV陽性檢出率爲23.68%（13森雨5/570），其中保育豬病料的陽性檢些林出率最高，爲32.24%（108/335）。同源性對(為對duì)比及遺傳進(jìn)化分析結果顯示，60個PRRSV均爲P我化RRSV2（美洲株），主要屬于譜系1和會購譜系8，占比分别爲51.67%和43.33%。氨基酸分析結刀事果顯示，60個PRRSV的GP5兵在蛋白氨基酸以點突變爲主，其中10個P微藍RRSV的GP5蛋白氨基酸出現缺失突變，且非中和表歌老位、中和表位、潛在毒力位點及Ｎ-糖基化位點均發(fā)生不同程度的變異。結果懂海表明，保育豬群爲PRRSV高發(fā)病群體，譜系校見1 PRRSV或已成(chén坐行g)爲國(guó)内流行優勢毒株，且PRRSV不斷發(fā)生變異，這又街(zhè)或許會(huì)影響現有疫苗的免疫保護效果。因此，針對(duì購煙)PRRS需要綜合防控，除選用安全有效章金的PRRSV弱毒活疫苗進(jìn)行免疫外，加強豬場生物安全及飼養管理也極其內讀重要。

Molecular Epidemiological Investig校吧ation on PRRS in 2020 in Some Region街藍s of China

In order to invest拿還igate the prevalence and不麗 molecular genetic variat雜說ion of porcine r志新eproductive and respiratory synd藍還rome virus（PRRSV）in some regions of會小 China in 2020，570 從筆suspected PRRSV inf場朋ected samples collecte好廠d from 7 provinc林拍es were detected by RT-自熱PCR，and the ORF5 gene 中司of 60 PRRSV positive sa為地mples were sequenced海雜 and analyzed. The results showed快答 that，by RT-PCR，the po厭生sitive rate of 山鐵PRRSV was 23.68%（135/570），and h年家ighest in nursing pigs，which was 車路32.24%（108/335）. It wa月線s shown that，by the results of是美 homology comparison and genetic evo畫鐘lution analysis，all the 60 PRRSV w日麗ere PRRSV2（American strai農頻ns）and mainly belonged爸學 to lineages 1 and 8，accounting for 5朋是1.67% and 43.33%，respectivel子老y. It was shown that，by the r資煙esults of amino aci技路d analysis，point mutations were d美員ominant in the GP5 protein amino a中行cid of all the 60 PRRSV，deletio樂日n mutations were observed in低到 10 PRRSV thereof，and variations wer妹好e observed in non-neutralizing epit藍城opes，neutralizing epitopes，potential術能 virulence sites and N-glyc電那osylation sites to d購照ifferent extent. In conclusion中說，nursing pigs were highl化鐘y susceptible to PRRSV. Lin鐘低eages 1 PRRSV might have b間請ecome a dominant stra金森in in China，and the immune protect樹裡ion effect of current vacc街拿ines might be affected by constantly購請 variation of the vi司術rus. Therefore，i物件n addition to vaccination wit麗為h safe and effect知輛ive attenuated li謝雪ve vaccines，biosafety and feeding ma森那nagement on farms had also played an術窗 important role in preventi冷火on and control o和慢f PRRSV.

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