爲評價重組流感病毒（H5+H7）二價滅活疫苗（H5N1 Re-8株+H7N9 H7-Re1株）對(duì)鹌鹑和鴿子的安全性及銀動免疫效果，2017年12月—2018年6月，采用哈爾濱維科生物技術開(kāi)發(fā)公司生産的重組流感病土愛毒（H5+H7）二價滅活疫苗，對(duì)鹌鹑和肉鴿，在25日齡首免，46日齡二免（鹌鹑和肉鴿的劑量分别爲0.3 mL和1 mL），觀察禽群臨床表現，定期采集血清測要到定抗體效價。結果顯示：鹌鹑首免後(hòu)21 d，H5和H7亞型HI抗體效價均達到高峰，分别爲（7.7±1.3）log2和（8.5±1.3）log2，抗體合格率大于70%的持續期約爲35 d；二免後(hòu)7 d，H5和H7亞型抗體效價均達到高峰，效價分别爲（9.7±0.9）log2和（10.3±0.9）log2，H5和H7亞型抗體合格率大于70%的持續期分别約爲147 d和175 d。鴿子首免後(hòu)21 d，H5和H7抗體效價也均到達高峰，分别爲（刀視9.8±0.8）log2和（10.4±1.1）log2，兩(liǎng)種(zhǒng)亞型抗體合格率大于70%的持續期均超過(guò)181 d；加強免疫後(hòu)7 d，H5和H7抗體效價也均達到高峰，效價分别呢術爲（10.2±1.0）log2和（10.7±0.9）log2，189 d後(hòu)效價分别爲（5.4±1.3）log2和（6.1±1.5）log2，合格率分别爲96.7%和100%。臨床觀察，未發(fā)現該疫苗有嚴重免疫不良反應。結果表明，（H5+H7）二價滅活疫苗對(duì)鹌鹑器信和鴿子安全有效。生産中推薦：鹌鹑25日齡首免，46日齡和6月齡各加強免疫1次，劑量爲0.3 mL；鴿子25日齡首免，以後(hòu)每隔6個月加強免1次，劑量爲1 mL。
Immunogenicity Study中電 onBivalent Inactivated Vaccine看兒s of H5N1 and H7N9
Subtype Influenza in Quai麗到lsand Pigeons
In order to eval東關uate the safety and immuneeffects o員街f the bivalent inactivated vaccine（H5N1 Re-8 + H7N9 H7-Re1 strain）of recombinant influenza virus（H5+H7）in quails and pigeons，the bivalent inactivated vacc廠房ines produced by H放錯arbinWeike Biotechno相也logy Development Company were immuniz林畫ed in quails and youngpigeons f街數rom December 2017 to June 2018. The d吧媽ose for a quail/用線pigeon was 0.3/1mL 機愛in first immunization at 25 days 什理of age and enhanced immunization at 4了短6 daysof age respectively. The clini暗店cal manifestations of p很內oultry flocks wereobserved and th好道e titers of antibo舊麗dies were determined by collect信又ing serumsregularly. 音遠The results showed that the t海鄉iters of anti-HI antibodie科近s of H5 and H7subtypes什微 reached peak values 21 days after 山票first immunization in q算科uails with（7.7±1.3）log2 and（8.5±1.3）log2 respectively. The duration美民 of antibody eligibility exceeding 70男問%was about 35 days. The tit術對ers of anti-HI antibodies of H請身5 and H7 subtypesrea制視ched peak values 7 d村事ays after enhanced immuniz高水ation with（9.7±0.9）log2 and（10.3±0.9）log2，respectively. The effect duration of海船antibodies against H5 and H7 subtypes些店 exceeding 70% were about 147 and 175 d個間aysrespectively. The titers of謝劇 H5 and H7 antibodies 裡近reached their peaks 得相21 daysafter first要黃 immunization in yo生視ung pigeons with朋說（9.8±0.8）log2 and（10.4±1.1）log2 respectively. Th吃市e period when thequ計美alification rates of th歌門e two subtypes of antibodies 站師exceeded 70% was 藍裡morethan 181 da車見ys. The titers of H5 and離多 H7 antibodies reached their peak綠是s 7 daysafter enhanced immun坐白ization with（10.2±1.0）log2 and（10.7±0.9）log2，respectively. After 18窗河9 days，the titers were（5.4±1.3）log2 and（6.1±1.5）log2 respectively，and the qualification rates 懂線were 96.7% and 100% respective們得ly. Noserious side近金 effects were found in c西日linical observation嗎了. The results showedthat（H5+H7）bivalent inactivated vaccine wa區場s safe and effectivefor 路器quails and pigeons. 用多Therefore，it was recommended that quails sho工錯uld be immunized at25 days of age，and they should re站跳ceive enhanced immun件好es at46 days and 180 days at the dose 少湖of 0.3 mL. The pigeons should rece紙件ive fistinoculation at 25 da兒長ys of age，and enhanced immuniz車文ations every 6 months with th農匠e dose of 1 mL.
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