爲了解僞狂犬病病毒（pseudorabies virus，PRV）放綠4種(zhǒng)主要毒力基因（gB、gC、gD和gE）的分個金子特征和變異情況，采用PCR技術，對(duì)20員科10—2015年分離到的28株P美理RV進(jìn)行基因擴增和測序。結嗎現果顯示：2010—2015年分離毒株的g少在B、gC、gD和gE基因均存在多位點突變，且位于抗光商原表位區；與2010年分離毒株的gD基因相比，2012年後(h山妹òu)分離毒株存在6個連續氨基酸插入。基于gB、gC、gD和g生熱E基因的進(jìn)化樹顯示：上海市2010年分離的4個毒株與2012年以間老後(hòu)分離的毒株雖屬于同一大的分支，但與經(jīng遠就)典毒株親緣關系近，處于同一小的分支中；2012年分離毒株與2011年後有生(hòu)國(guó)内變異株親緣關得又系近，處于另一分支中。這(zhè)些毒力基又又因抗原表位區域氨基酸的突變可能(néng)導緻其毒力及抗原業森性發(fā)生改變；2012年以後(hòu)分我錢離毒株均爲變異株，并已成(chéng)爲上海市的主要流黑不行毒株。本研究爲PRV分子緻病機制及分子流行病學(xué)研究離放提供了依據。

Molecular Variation Analy子唱sis on Virulence Genes Re物慢lated to

Pseudorabies Vi男頻rus in Shanghai City

In order to identify 錢都the molecular cha拿報racteristics and v匠鐵ariation of majo問音r virulence genes（gB，g音吧C，gD and gE）of pseudorab車劇ies virus（PRV），28 strains isolated fr秒飛om 2010 to 2015 were amplified and seq志場uenced by PCR ass車計ay. The results理技 showed that multi-site mutat姐草ions appeared in the epit筆愛ope zones of all the genes；comp科你ared to the gD gene isolated in 去煙2010，six consecutive amino aci爸學d insertions appeared in the strains 就鐵isolated since 2012. 雪腦Based on the genetic evo冷窗lution trees of g術商B，gC，gD and gE gene，the four strains 作林isolated in 2010 belon銀員ged to the same big branch as 秒來the strains isolate刀厭d since 2012，but they were closely rela會睡ted to the classica現雨l strains，which were in the sam司著e small branch；the strains isolate相算d in 2012 were closely r歌如elated to the domest雪站ic variant stra跳就ins isolated since 2011，which 購快belonged to another branch. The virul如體ence and antigen自內icity of the genes might be c很黑hanged by the muta微你tions of amino aci務時ds in the epitope zones. All the st年資rains isolated since 20影分12 were variant，and h到樹ad become prevalent in Shanghai 日視City. Therefore，some referen一空ces were provided for the st什窗udy of PRV molecular pathogenesi我費s and molecular epidemiology.

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