豬瘟（CSF）是由豬瘟病毒（CSFV）感染豬引起(村麗qǐ)的一種(zhǒng)急性、熱性、接觸性傳染病，嚴重危害我國(g和內uó)養豬業健康穩定發(fā)展。本研究采用RT-PC慢月R方法，對(duì)從雲南省甯洱縣2頭病死野豬體内分離到的2株黑校CSFV毒株進(jìn)行了E2及5'NTR基因擴增、克隆及序列術裡測定；采用DNAMAN、MEGA6等分子生物學(xué)軟件，對(du器數ì)測得的序列與國(guó)内外參考毒株進(jìn)頻器行了同源性分析及系統發(fā)育分析。結果顯示：2株野豬源CSF民現V株E2、5'NTR基因同源機煙性分别爲87.2%、100%，與國(gu計熱ó)内外參考毒株同源性分别爲81.0%~97.6%、93.6%~98得師.5%，與我國(guó)強毒株友輛Shimen株的同源性分别爲81水媽.0%~81.2%、95.6%；2株野豬源CSFV毒株E2基因屬習風于基因2.1亞型，5'NTR基因屬于基因2.坐用3亞型。氨基酸序列分析顯示：其中一株分離毒株的E2基因有6個氨基酸具有理劇2.1d分子變異特征，另一株兼有2.1d和2.1b亞型分支特征鐵窗，可能(néng)是2.1b和2.1d亞型的過(guò)渡毒株。結年醫果表明，雲南省野豬源CSFV雖存在一定的遺傳衍畫人化，但總體比較穩定。本研究爲雲南省CSF防控提靜著供了分子流行病學(xué)依據，爲進(jìn通動)一步做好(hǎo)分子變異等研究奠定了基礎。

Sequencing and Analysis of E2 and 5&#讀多39;NTR Genes of CSFV Derived from W物子ild Boars in Yunnan Province

Classical swine f哥還ever（CSF）is an acute就微，febrile and contact infect又水ious disease cause妹輛d by classical swine fev南資er virus（CSFV），which has 物會seriously endangered the要會 healthy and stable development 男如of pig industry in China. In this stud場購y，E2 and 5'NT玩白R genes of two CSFV strains isola森就ted from two dead wild boar放唱s in Ning'er County of Yunna從話n Province were amplified，大我cloned and sequenced b船歌y RT-PCR；the homology and phyloge女謝ny of the obtained sequences and the區藍 reference strain多電s were analyzed a去她nd compared by DNAMAN，M玩計EGA6.0 and other molecular 森多biology software. The r生議esults showed that the homology of E2 到件and 5'NTR ge化算ne of the two strains were 87.2% and 10草我0%，respectively，which were什輛 81.0% to 97.6% and 93.6% to 9下拿8.5% respectively with the re務都ference strains，81.0% to就麗 81.2% and 95.6% respectively with熱如 Shimen virulent strains in Chi事姐na；for the two 場明strains，E2 gene and 5'兵亮;NTR gene belonged to subtype 2服亮.1 and subtype 2.3，respectiv哥化ely. As indicated by a音低mino acid sequence analysis，6 靜紙amino acids with molecular variat和知ion characteristics大訊 of 2.1d subtype were observed in one s不和train，characteristics of both雪腦 2.1d and 2.1b subtyp器市es were in another strain，which北作 might be a transitional strain o鐘日f the two subtypes. In conclusion，the C廠去SFV prevalent in wild boars in Yunnan 長朋Province was gener雨明ally steady although a certain g一購enetic evolution was de但可tected. A molecular epidemiolo對志gical basis was provide器坐d for prevention and control of CSF雨舊 in Yunnan Provi文市nce，and a foundation wa劇厭s also laid for further studies on m得可olecular variation.

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