目前關于規模化驢場主要病原的流行數據極爲有限。爲了解市能山東省驢養殖密集區的主要病原流行情況，2019—2021年，從森又聊城、德州、濱州等地的9個規模化驢場（繁育母驢100頭以上子學），無菌采集表現不同臨床症狀病死驢（駒）的心、肝、脾、肺、腸、腎等不同髒水船器病料共計742份，采用PCR與病原下這分離方法進(jìn)行常見馬屬動物病原檢測；采集296份臨床健康驢血木白液樣(yàng)品，采用虎紅平闆凝集試驗進(jìn)行布魯氏菌感染抗體通一檢測。結果顯示：在腹瀉症狀樣(yàng)品中，大腸杆菌檢見些出率最高，爲48.89%（13謝西2/270）；在肺炎症狀樣(yàng用森)品中，大腸杆菌檢出率爲24.10%（80算離/332），肺炎克雷伯氏菌檢出率爲29.82%（99/332），馬鏈球菌檢出工讀率爲22.29%（74/332）；在流産症狀樣(yàng)品中，沙門氏女事菌檢出率最高，爲44.29%（62/140）；在臨工吧床健康驢血液樣(yàng)品中，未筆說檢出布魯氏菌感染抗體。結果表明，導緻規模驢劇信場發(fā)生腹瀉、肺炎和流産的主校商要是條件性緻病菌，其中導緻腹瀉老風的主要是大腸杆菌，肺炎的主要是大腸杆菌、肺炎克雷伯氏菌和馬鏈球窗火菌，流産的主要是沙門氏菌。因此，規模化驢場應舊現通過(guò)加強飼養管理，提高養殖圈舍衛生水平，科學(xué)使用抗生請答素等措施，控制細菌性疫病流行。
Investigation on Major Pathoge子事ns in Large-scale D頻線onkey Farms in Shandong Province
As there had bee劇生n very limited data on the 要票prevalence of major pathogens in弟公 large-scale donkey farms，in著中 order to identify the infection stat坐務us in the intensive breeding are購跳as in Shandong Province，from 2019 to厭媽 2021，a total of 742 samples of les一麗ions including hearts，l在睡ivers，spleens，lungs，int討但estines，kidneys and other和到 organs of dead donke術上ys（foals）with differ她秒ent clinical symptoms were 爸人aseptically collected from 9 farms（w匠飛ith above 100 female don暗光keys）in Liaocheng，Dezhou討冷，Binzhou and other regions to detect 冷照common equine pathogens b務男y PCR and pathogen isolation method厭章；and 296 samples 是理from clinical healthy donkeys to det銀司ect the antibody a見見gainst Brucella by RBT. The results 那能showed that，for the samples with d什訊iarrhea symptoms，t草內he detection rate of Escherichia 廠月coli was highest，which was 48筆做.89%（132/270）；for thos妹煙e with pneumonia symptoms，the detec短有tion rates of Escherichia coli，Klebsi熱厭ella pneumoniae and Strept時近ococcus equi were 24.10%（80/332），29.吧媽82%（99/332）and 22.29%（74/33為睡2），respectively；f慢風or those with abo議相rtion symptoms，the detection rate of 土土Salmonella was 這校highest，which was 44.29%（62/140）；and no輛什 antibody against Brucella wa林老s detected in the 她兒blood samples of clinical hea場在lthy donkeys. In concl數自usion，the diarrhea，pneumoni鐵筆a and abortion occurred in the farms w我會ere mainly caused by conditional 你頻pathogens，specifica相森lly，diarrhea was mainly caused by Esch吃麗erichia coli，while pneumonia by Esc地事herichia coli，Klebsiella pneumoniae an農風d Streptococcus equi，土器and abortion by Salmonella. Therefore，書廠bacterial diseases should b慢了e prevented and controlled throug高服h strengthening fee少爸ding management，improv在費ing the health level of pen西員s，using antibiotics scientifically and師是 other measures.
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