近年來，禽呼腸孤病毒（ARV）在我國(gu他光ó)白羽肉雞群中的感染率呈上升趨勢，流舞著行範圍較廣，給我國(guó)肉雞養殖企業帶來了較大經(jī少還ng)濟損失。ARV易變異重組，因此全關你面(miàn)了解其分子流行病學(xué)特征謝吧具有十分重要的意義。爲了解我國(guó)白羽肉雞群中ARV的遺傳變異特點，20綠木20—2021年從山東、河北、福建、安徽、遼甯、江蘇等6個省份收但他集疑似ARV感染樣(yàng)品，采用RT-PCR方法篩選陽性樣(yàn厭不g)本，然後(hòu)對(duì)陽性樣(yàng)本進黑海(jìn)行σC基因（1 088 bp）測序，根據門讀測序結果分析ARV不同基因型分布規律及占比。結果顯示：2020—2021年，路相從6個省份中，經(jīng)RT-PCR檢測，檢出ARV陽性樣(yàng)品1中話57份，總陽性檢出率爲62.1%，不同省份的陽性檢出率爲紅男44.4%~77.3%。157份陽性少看樣(yàng)品可分爲6個基因型，其中基因1型61份（38.85%）、民音基因2型41份（26.11%）、基因3型15份（9.56%）、大坐基因4型9份（5.73%），基因5型30份（19.11%），基因6型1份（0.北睡64%）；61份基因1型樣(yàng)品中，讀票38份與标準ARV疫苗株（S1133）森煙屬于同一亞分支，另外23份與4599參考株處于單獨的另一亞分支。結果表劇民明：我國(guó)肉雞群中存在一定程度的AR訊東V流行；流行的ARV基因型較爲複雜，且存在基因1型變異毒株，當前的疫苗株已舞現不能(néng)對(duì)其提供足夠的保市厭護。結果提示，今後(hòu)需要進(jìn)區放一步開(kāi)展ARV感染監輛門測，評估其流行及變異情況，調整免疫策略，加快新疫苗等防疫技術及産子靜品研發(fā)。本研究初步了解了我國(guó)部分省份的ARV分子流行到房病學(xué)特征，對(duì)我國(guó)ARV感染的防控具有拍路重要意義，也爲變異株新型疫苗研發(fā)提供了依據。
Molecular Epidemiological Inve畫國stigation on Avian Reovirus in Some就綠 Provinces of China from 2020 to 雨報2021
In recent years，avia國答n reovirus（ARV）has be大上en increasingly and widely p懂技revalent in white feather br木她oilers in China，bringi從呢ng great economic losses秒不 to broiler breeding enterprises 計輛in China. As ARV is prone to mut算森ation and recombination，it wou民木ld be of great significance 們和to comprehensively investigate 紅中its molecular epidemiological cha她窗racteristics. In 生船order to investigate the ge通道netic variation characteristics of做自 ARV in white feather 區短broilers in China from 為鐘2020 to 2021，253 suspected ARV inf時體ected samples were co民拍llected from Shandong，Hebei近森，Fujian，Anhui，Liaoning and Jiangsu pr子樹ovinces to detect positive samples by 的工RT-PCR，followed by σC輛看 gene（1 088 bp）sequencing，ag微也ainst which，the西日 distribution and proportion of城他 different ARV genotypes他遠 were analyzed. The results sho行為wed that 157 positive samples we地時re detected by RT-PCR，with a tota農哥l positive detection rate 藍很of 62.1%，and that in each pr看文ovince was from 44.4% to 77.多銀3%. All the 157 posi紙電tive samples were di服暗vided into 6 genotypes，including 61 g還醫enotype 1（38.85%），41 genotype 2（26.11來裡%），15 genotype 3（9醫樹.56%），9 genotype 4（5.7習都3%），30 genotype 物愛5（19.11%），and 1 genotype 美雨6（0.64%）；38 out of 61 genotype 1 sa雜見mples belonged to the sam木快e sub-branch as th用友e standard ARV vacc也為ine strain（S1133），an票生d the other 23 fell i房議nto a separate sub-b動懂ranch with 4599 refe數年rence strain. In conclus習森ion，ARV was prevalent in 內藍broilers in China 房們to certain extent；the prevalent AR商花V genotypes were comple能問x，and there were genotype 1 河男variant strains，so suffici草爸ent protection c月工ould not be provided by cu飛人rrent vaccine strains anymore，which 些家indicated that survei快海llance for ARV infec資通tion should be furth和靜er strengthened，its prevale這說nce and variation should be evalu女著ated，immunization strategies sh城視ould be adjusted，and research and de黃用velopment of disease pre還生vention technologies and p司一roducts such as new vaccines s吃街hould be accelerate文舊d. The above study on the molecular妹歌 epidemiological characteristics of ARV影票 in some provinces of China supported t說愛he prevention and control of ARV inf廠刀ection in China，and provided a 數就basis for the research and develo放的pment of novel v爸南accines against any variant s低都trains.
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