爲評價重組流感病毒（H5+H7）二價術放滅活疫苗（H5N1 Re-8株+H7N9 H7-Re1株）對(duì)鹌鹑一空和鴿子的安全性及免疫效果，2017年12月—2018年6月，采用哈湖唱爾濱維科生物技術開(kāi)發(fā)公司生産的車費重組流感病毒（H5+H7）二價滅活疫苗，對(duì)鹌鹑和肉鴿，在25日齡綠如首免，46日齡二免（鹌鹑和肉鴿的劑量分别爲0.3 mL和1 mL計她），觀察禽群臨床表現，定期采集血清測定抗體效價。結果顯示：鹌鹑首免後照鐵(hòu)21 d，H5和H7亞型HI抗體效價均達到高峰，分别爲對話（7.7±1.3）log2和（8.5±1.3）log2，抗體合格率大于70謝跳%的持續期約爲35 d；二免後(hòu)7 d，H5和H7亞型抗體效價黑喝均達到高峰，效價分别爲（9.7±0.9）log2和（10.3微黃±0.9）log2，H5和H7亞型抗體合格書村率大于70%的持續期分别約爲1問很47 d和175 d。鴿子首免妹坐後(hòu)21 d，H5和H7抗體效價也均到達高峰，分别爲（9.8±0近湖.8）log2和（10.4±1.1）log2，兩(liǎng)公睡種(zhǒng)亞型抗體合格率大于70%的持續期亮吃均超過(guò)181 d；加強免疫後(hòu)7 d，H5和H7票女抗體效價也均達到高峰，效價分别爲（10.2±1.0）log2和朋木（10.7±0.9）log2，18鐘場9 d後(hòu)效價分别爲（5.4±1.3答吧）log2和（6.1±1.5）log2，海家合格率分别爲96.7%和100%。劇小臨床觀察，未發(fā)現該疫苗有嚴重免疫不良反應。結果黃器表明，（H5+H7）二價滅活疫苗對(duì)鹌鹑和鴿子安全哥慢有效。生産中推薦：鹌鹑25日齡首免，46日齡分笑和6月齡各加強免疫1次，劑量爲0.3 mL；鴿子25日齡首免，以後(hòu)每舞日隔6個月加強免1次，劑量爲1 mL。Immunogenicity Study 你學onBivalent Inactivated Vaccines of 地放H5N1 and H7N9Subtype 樂刀Influenza in Quailsand Pi得習geonsIn order to evaluate the safe計科ty and immuneef間道fects of the biv慢也alent inactivated 得店vaccine（H5N1 Re-8 + H7N9 H7信姐-Re1 strain）of recombinant influenza v商舊irus（H5+H7）in quails a議歌nd pigeons，the bivalent inac是票tivated vaccines produced by Harbin商電Weike Biotechnology Development Comp電制any were immunized in 章費quails and youngpige服友ons from December 2017 to June 2018. 為醫The dose for a quail/p城畫igeon was 0.3/1mL in 讀冷first immunization at 他畫25 days of age and 睡校enhanced immunization又還 at 46 daysof age respect務術ively. The clinical小聽 manifestations些件 of poultry flock兒飛s wereobserved a跳身nd the titers of antibodies 坐醫were determined by collecti自笑ng serumsregular通木ly. The results showed that the tite們雜rs of anti-HI anti機媽bodies of H5 and H7subtypes reached 制筆peak values 21 d公麗ays after first immunization in qu和照ails with（7.7±1.3）log2 and（8.5±1.3）l外海og2 respectively煙子. The duration of antibody eligibi問子lity exceeding 70%was a開說bout 35 days. Th國月e titers of anti-HI antibodie事廠s of H5 and H7 subtypesreached peak算計 values 7 days after店拿 enhanced immuniza微歌tion with（9.7±0.9）log2 and（10.3±0.9）暗秒log2，respectively. The effect durat公微ion ofantibodies姐和 against H5 and H7 subtype制音s exceeding 70% were about 雨微147 and 175 days多長respectively. The ti公土ters of H5 and H7 antibodies reached 男去their peaks 21 daysafter first吃請 immunization in young p金日igeons with（9.8±0.8）l村道og2 and（10.4±1.1）log村文2 respectively. The period 秒動when thequalific愛友ation rates of the two 音說subtypes of antibodies exceeded 70%視現 was morethan 181 days. T一中he titers of H5 and H7 an刀男tibodies reached their peak不明s 7 daysafter enh相短anced immunization with（10.2±1.0）lo機做g2 and（10.7±0.9）log2，re些影spectively. After 18文信9 days，the tite亮司rs were（5.4±1.3）log2學公 and（6.1±1.5）log2 r嗎時espectively，and t工冷he qualification rat草秒es were 96.7% and 100% r飛子espectively. Noserious s花廠ide effects were found in clinica日算l observation. 線他The results showe自吃dthat（H5+H7）bivalent藍雨 inactivated vac人裡cine was safe and effectivefor qu下很ails and pigeons. Therefore，it w書請as recommended tha暗請t quails should be im劇很munized at25 days of age，an草什d they should rec對嗎eive enhanced immune文煙s at46 days and鄉草 180 days at the dose of 0照妹.3 mL. The pigeons should刀謝 receive fistinoculation at學綠 25 days of age，and enhanced immunizat姐老ions every 6 months wi計生th the dose of 1 mL. 全文下載鏈接空人：http://kns.cnki.net/kcms/detail/37.1好冷246.S.20190222.0911.030.html?科著uid=WEEvREcwSlJHSldTTEYzU3Eyd又國DRPZTFBeXhDa29ZZ2ljVUtvTXF2T房煙3dtND0=$9A4hF_YAuvQ5obgVA花自qNKPCYcEjKensW4IQMovwHtwkF4VYPoHbK我通xJw!!

2019-03-04