2021年雲南省玉溪市小反刍獸疫專項監測評估
爲全面(miàn)掌握雲南省玉溪市小跳公反刍獸疫（PPR）的病原分布和羊群免答國疫效果，根據2021年玉溪市PPR專項監測方案，在全市白地開(kāi)展PPR監測和流行病學(xué)調查。采家吃用多階段調查的方法，從全市9個縣區采集免疫羊血清樣(yà制時ng)品1 631份、眼鼻棉拭子樣(yàn懂內g)品6 438份，分别使用阻斷ELISA子作和實時(shí)熒光RT-PCR檢測方法，進(jìn)行P慢錯PR免疫抗體檢測和病毒核酸檢測。結果顯示：2021年玉溪市PPR群體免疫合格率土會爲82.98%，個體免疫合格率爲83.62%，未檢測到病原學(xué)陽性樣(師城yàng)品；不同養殖區域、不同性别的免疫抗有拍體合格率均有統計學(xué)差異（P術路＜0.05），而不同養殖類型、不同企業疫苗的免疫抗體合格率差異均不顯著（P笑你＞0.05）。結果表明：玉溪市羊PPR整體免疫效果較好(hǎo)，所用PPR在你疫苗免疫原性較好(hǎo)，免區站疫程序合理，但部分縣區的免疫合格率較低，因此應繼續加強PPR的強制免疫及補麗工免工作；在未引入新疫源的前提下，疫情發(fā)生風險較低，物有但應加大羊隻引種(zhǒng)檢疫和調運監管力黃電度。本次監測評估了玉溪市的PPR總體防控狀況，爲盡輛國快實現消滅PPR的目标提供了數據支撐。Special Monitorin新就g and Evaluation綠可 on PPR in Yuxi City of Yunnan Provinc要裡e in 2021In order to invest如會igate the distribution of pathogen 什匠of peste des pe她唱tits ruminants（PPR）and immun媽友e effect in sheep in Yuxi City of Yu月化nnan Province，targeted monitoring and如一 epidemiological investigation were車如 carried out across the city 話個according to the special monitorin中自g plan for PPR published in the cit得美y in 2021. A total of 1 631 serum sa雜土mples and 6 438 eye or nose日訊 swabs were colle海懂cted from immunized sheep 書森in 9 counties/districts in the city by地自 using multi-stage investigatio子員n to respectively detect immune antibo也也dies against PPR and virus用相 nucleic acid by bl但黃ocking ELISA and real-time fluorescen資術t RT-PCR. The results showed th信去at the qualified rates of火農 population and individual animal w術家ere 82.98% and 83.62%，respec多金tively，and no positive少來 samples were d匠放etected；the qualified rates of imm一作une antibodies in different breeding 答和regions or different gen什筆ders were of sig數明nificant statistical diffe行很rence（P＜0.01），but slightly 風雪in different breeding types and vaccine民些s produced by different ente放技rprises（P＞0.05）. In concl生算usion，the overall immunizat笑很ion effect against PPR was goo雪我d in the city，with good immunogenici業得ty of PPR vaccines as w紅玩ell as reasonable immunization 鐘司procedure，but the qualified光作 rate of immunity w員匠as lower in some counties/districts，wh關冷ich should continue to im資厭plement compulsory or suppleme線說ntary immunization agai木嗎nst PPR；and sheep to be i道新ntroduced should be s你雪trictly quarantined a離鐘nd supervised during transportation謝在，although the risk of outbreak was lo她呢wer in the context of absence of any章這 infection source. The general 我通status of prevention and contro理請l of PPR in the ci學一ty was monitored 我船and evaluated in the paper，fr做習om which a reference was provided我一 for reaching the 我體goal of eliminating PPR soonest.全文下載大秒鏈接：https://chn.oversea.cnki.ne月冷t/KCMS/detail/detail.aspx?d媽湖bcode=CJFD&dbname=CJFDAUTO&file國也name=ZGDW202206003&un街問iplatform=OVERSEAS_CH購說S&v=vPzqR5W2eDA5b6bairyzxI-xcmvfp員體BTPr92rdTmp3JGk行數DchVhYZ2Sp5PHkZpC外新Es0
2022-06-17
湖北省部分規模豬場豬繁殖與呼吸綜書和合征流行病學(xué)調查
爲探究豬繁殖與呼吸綜合征病毒（PRRSV）在規模豬場的流行特征，2兒書021年從湖北省7個規模豬場疑似PRRSV感染豬群以及所有種(zhǒ資南ng)公豬群中，共收集樣(yàng)品3 324份，采用三重巢式RT-P近劇CR法進(jìn)行PRRSV病原學(xué)檢測去男，分析不同養殖類型、不同生長(cháng)階段、不同季節及不同感染毒他是株的流行特點，并將(jiāng)陽性産物進(jìn)行測序和遺傳進(jìn)化你內分析。結果顯示：3 324份樣(yàng)品中共檢測出PRRSV陽性樣身煙(yàng)品323份，陽性檢飛河出率爲9.72%（323/3 324）美費。育種(zhǒng)場陽性檢出率最低，爲2.66%金生（20/752）；商品豬擴繁場與育肥場陽性檢出率相近，分别爲1數子1.82%（169/1 430）與11.73%木兒（134/1 142）；保育豬群陽性檢出率最高，爲17.23%（152/8那好82）；其餘依次爲生長(cháng)育肥豬、哺乳仔豬和母豬，陽性檢出率答朋分别爲11.30%（121/1 071）、5.26%（30/5空海70）和3.24%（20/618）；種(zhǒng)公豬中未發(fā)現司不PRRSV感染。PRRSV一年四季均可流行，春、夏、秋、冬季的陽性檢出地黃率分别爲18.16%（148/815）、6.94%（60/妹市864）、6.10%（34/557）、7.44%（81/1 0還作88），春季陽性檢出率顯著高于其他季節（P＜0.05），而夏了拿、秋、冬季節間差異不顯著（P≥0.05）。3種(zhǒng)P票愛RRSV毒株均有流行，類NADC30毒株、海遠經(jīng)典毒株和高緻病性變異毒株的占比分别爲4年分9.54%、21.36%、29.10%，類NADC30長北毒株檢出占比顯著高于其他類型毒株（P＜0.05）。結果表明：PRR白你SV在所調查的7個規模豬場中流行普遍，尤其是商品豬擴繁場與育肥場樹相；保育豬群PRRSV感染風險較高，種(zhǒng)公豬感染風險較低，春季是高發術現(fā)季節，類NADC30毒株爲優勢流行毒株。結果提示，規模養殖場應根麗光據PRRSV的流行特征，加強免疫和監測，有針對(duì)性地制月風定防控措施。Epidemiological Investiga匠喝tion on PRRS in Some Intensive Swin討答e Farms in Hubei Prov訊你inceIn order to investigat日東e the epidemiological character什動istics of porcine 說放reproductive and respiratory synd懂媽rome virus（PRRSV）in intensive swine f廠去arms，a total of 3 324 samples were 劇我collected in 2021 from房的 pigs suspected to b冷見e infected with PRRSV 分服and all boars in黃光 7 intensive far內房ms in Hubei Pro為窗vince for pathogenic de說不tection by triple-nested會我 RT-PCR to analyze the prevalent situa場西tion in different bre關話eding types，growth stag自間es，seasons and strains，followed筆樹 by sequencing and genetic ev現兒olution of positive身麗 products. The r員科esults showed th件少at 323 positive samples were det道會ected，with the positive rat農算e of 9.72%（323/3 324）；村議the lowest positive detection 鐵就rate was 2.66%（20/752）in b資志reeding farms，sim和機ilar in commercia森木l propagation farms and fat熱志tening farms，whic厭城h were 11.82%（169/1 430）他來and 11.73%（134/1 142）respectiv道聽ely；highest in nursery pigs（17.23%），媽低followed by gro多林wing finishing pigs，suck少著ling piglets and sow廠窗s，with 11.30%（121/1海事 071），5.26%（30/570）and 3.24%草視（20/618）respectively，no PRRSV infectio不微n was found in breeding boars；PRRSV c物呢ould be prevalent throughout a 商他year，and the positive de地空tection rates in spring，summ坐森er，autumn and winter we藍南re 18.16%（148/815），遠答6.94%（60/864），6.10很自%（34/557）and 7.44%（81/1 08通農8）respectively；th答如at in spring was significantl低視y higher than those i會窗n other seasons不他（P＜0.05），but with slight視頻ly difference in summ暗拿er，autumn and winter（P≥0.0計場5）；all three types of strain讀煙s including NADC30-like strain，class事弟ical strain and highly pat高務hogenic variant strai器廠n were prevalen嗎腦t，accounting for 49.54%，21.36% and 29個坐.10%，respectively，the土他 proportion of NADC30-like水就 strain was signifi關河cantly higher than other t道河ypes of strains（P＜0.05）. In坐藍 conclusion，PRRSV was widel樂長y prevalent in the 7 花門farms，especially in commer分但cial propagation farms and fatte關美ning farms；the risk of 少飛PRRSV infection was high in nursery pi書鄉gs，lower in breeding boars；新日and NADC30 like strain was the 著秒dominant epidemic strain，esp火了ecially in spring.北大 Therefore，immunization and黑請 monitoring should be s明唱trengthened by the farms 街長according to the prevalent 工你characteristics of PRRSV，個輛and targeted meas房上ures for prevention and control shoul民綠d be developed.全文下載鏈接：https:資兒//chn.oversea.cnki.電嗎net/KCMS/detail/detail.aspx?很資dbcode=CJFD&dbname=CJFDAUTO&filename=Z書對GDW202206002&uniplatform=OVERSEAS_CHS&v笑中=vPzqR5W2eDDKKGaM暗低rEXpotlTh1oYw2Bm_YAkX9Gholoy7ec2RZen綠機diMer1XFc86Y
2022-06-17
四川省規模場豬僞狂犬病定性風險評估
爲評估四川省規模場豬僞狂犬病（PR）發(fā)生風險，通過(guò)問卷調查舞外了解省内種(zhǒng)豬場、擴繁豬場、商品豬場的生産管理情況，用情景樹法描熱電述PR發(fā)生的風險路徑，在世界動物現年衛生組織（OIE）風險評估框架下構建評估模型。筆畫根據問卷調查結果和本底調查數據，采用定性風險評估方式，用矩行舊陣法估算各風險路徑的風險值。結果顯示，四川省規模場店窗發(fā)生PR的高風險路徑爲引林頻種(zhǒng)、使用精液、未有效控制鼠害、未有效控制犬貓以日樂及地理位置和飲水控制。近年來由于生物安全管理水平提高，經做多(jīng)人、車、物、料等傳統途徑釋放PR的風險大大知不降低，在各類型規模場開(kāi)展PR淨化的條件已較爲成(chéng)熟上子。在PR淨化的同時(shí)開(kāi)展風險評估我劇，能(néng)幫助豬場找出風險漏洞，便于藍司提出針對(duì)性的防控建議，爲P黑議R淨化提供有力的技術支撐。Qualitative舊下 Risk Assessment on Pse木放udorabies in Intensi年技ve Swine Farms in Sichuan Provinc雨女eIn order to assess possible risk of oc算多currence of pseudorabies（PR）in i錢人ntensive swine 機學farms in Sichuan Province，a拿了 questionnaire investigation was行窗 organized to i業玩dentify the status of producti地放on and management in b為關reeding farms，propagatio校木n farms and com外樂mercial farms in the p又鐵rovince，risk paths of occurre年吃nce of PR were described by a scen了雪ario tree，and an assessment model was銀志 constructed within the risk evaluatio弟業n framework proposed by OIE. B舊數ased on the results of investigation 西說and background survey dat也線a，the risk value of each risk 姐知path was estimat他務ed by a matrix method through a quali資去tative risk assessment. The r下森esults showed that the音化 high-risk paths of PR in the intensive線林 farms in Sichuan化光 Province included introduction of視商 animals，use of semen，ineffect河輛ive control of rode用志nts，dogs and cats，geo電草graphical locati船費on and control of drinking water. The黑通 risk of PR transmission via personnel暗員，vehicles，supplies，materials a身姐nd other traditional pathways was樹秒 greatly reduced due to improv大子ement of bio-safety management lev算錢el in recent years，and the東她 conditions for PR purification器理 in various intensive farms hav和答e been relatively mature算視. Risk assessment in combi習笑nation with PR purification could hel有呢p farms find out thei信花r own shortcomings related to r要筆isk and put forward targeted sugg得森estions for prev林鄉ention and cont木輛rol of the disease，which cou開章ld provide strong technical習討 support for PR purification.全文下問文載鏈接：https://chn.ove我章rsea.cnki.net/KCMS/det她用ail/detail.aspx?dbcode=花服CJFD&dbname=CJFDAUTO&filenam南一e=ZGDW202206001&uniplatform=OVER工化SEAS_CHS&v=vPzqR5W2eDAL從路qbekGSBGAi6JZgd7yqe劇謝4fN9btKxgvji3ih__y3CaL-pUUHrmifL事分W
2022-06-17
豬非典型瘟病毒（APPV）研究進(jìn)展
豬非典型瘟病毒（atypical p商的orcine pestivirus，A理從PPV）是2015年新發(fā)現的瘟病毒屬病毒，國男但(guó)際病毒分類學(xué)委員會(huì)2017 年將(jiāng頻了)其确定爲瘟病毒屬K（Pestivirus 紅對K）的代表毒株。該病毒可導緻仔豬先天性震顫（con腦自genital tremor，CT），典型症狀爲仔費讀豬輕微震顫，頭部和四肢明顯。目前APPV已在全信討球廣泛流行，我國(guó)也有相關報道(dào)，關于該病毒研究的嗎開報道(dào)越來越多，但研究進呢到(jìn)展的報道(dào)相對(duì)明上較少，目前已證實其與CT緊密相關。APPV不容易被(b校睡èi)分離，缺少最重要的病毒攻毒試驗結果，疫苗研究也需要在前期基礎研究深入的時村基礎上才有可能(néng)獲得成(ché問跳ng)功。爲此，本文從病原學(xué)、流行病學(xué)、診斷技術及器南疫苗研發(fā)等方面(miàn)，對(duì)取得的最新研究進(jìn)展進地大(jìn)行概述，以期增強人們對(duì)AP體低PV的認識，爲我國(guó)防控與研究APPV提供參考。Advanc什有es in the Researches on Atypical 光現Porcine PestivirusAtypical po請中rcine pestivirus（APPV）商自is a new pestiv舊船irus virus firstly 購妹detected in 2015，which is d南影etermined as the 相輛representative strain of pes路和tivirus K by the International Comm歌姐ittee on the Taxonomy of Viruses花謝 in 2017. Becau能議se of the virus，congen國錢ital tremor（CT）mi書吃ght occur in piglets with such typica村店l symptoms as slight 服高tremor especially in head an光用d limbs. APPV has been w讀分idely prevalent all over the worl會學d and also reported in China小現，but there are few reports光靜 related to the advances冷間 in relevant researches on the viru爸雪s although with more reports about 錯自the researches. A如也t present，it has be鄉鐵en confirmed to be closely relat土好ed to CT. It is difficult to isolate t為子he virus due to the lack of 術低most important results a微公bout virus attack test，and the develop黑輛ment on vaccines cou劇冷ld be achieved only ba民樹sed on the results 拍費of preliminary researches. The海動refore，the latest聽票 relevant advances were summari裡南zed from the aspects of etiology，epid動從emiology，diagnostic techno慢木logy and vaccine development，w對我ith a view to improving public'拍紅;s awareness for the vir讀新us and providing a referen的購ce for relevant prevention，co得黃ntrol and researches in China.全文下載鏈接：h明兒ttps://kns.cnki.net/kcms/detail/detail算民.aspx?dbcode=CJFD&d票著bname=CJFDAUTO&filename=Z花機GDW202205016&uniplatfo在地rm=NZKPT&v=vPzqR5W2eDDtiw71MCs喝雨ANnwey_G_1La7qGi家間a53jHi1KSbi1i4gGW上愛qNRZp8Hdwoqn
2022-06-07
mRNA疫苗免疫學(xué)機制研究進(jìn)展
信使核糖核酸（mRNA）疫苗屬于第三代疫苗，具有抗原表達效率高近如、安全性高、免疫原性強、可編譯性強，以及制備工藝簡單、易規模化雨機生産和針對(duì)病原體變異有效性高等技術優勢，在新冠疫情防控中被船書(bèi)視作一種(zhǒng)革慢下命性的疫苗技術。但mRNA 疫苗穩定性差，傳遞效率低，在臨床應用方面(miàn拍山)受到很大限制。因此，當前及未來的研究時黑主要集中在保證疫苗安全性的前提又關下，設計先進(jìn)和可耐受的遞送系統，來房醫提高抗原表達和提呈的效率，以及優化mRNA結構，達到延長(ch男線áng)和控制表達持續時(sh懂銀í)間的目的。本文從mRNA疫苗的分類、作用機制、技術優勢、遞送系統4動站個方面(miàn)，對(duì)mRNA疫苗免疫學(xué)木服機制研究進(jìn)行綜述，旨在爲mRNA疫苗研發(fā)工作光看提供參考。Advances i明也n the Immunological Mechanis慢線m of mRNA VaccineMessenger ribonucle雜西ic acid（mRNA）vaccine，大資as the third generati知業on vaccine，is char師房acterized by high ant愛快igen expression efficiency還站 and safety，strong i外技mmunogenicity and 商唱compiling，simple preparation 嗎來technology，intens制老ive production and high effici光鐵ency against pathogen variation，and ha哥日s been regarded as a revolutionary vacc海場ine technology in亮服 the prevention and control of COVID-1你醫9. However，the mRNA vaccine is with poo少關r stability and門好 low transmissio算微n efficiency，which is greatl黑火y limited in clinical計師 application. Therefore，a煙自dvanced and tolerable delive秒快ry systems should be designe是相d to improve the ef那坐ficiency of antige醫化n expression and presentation，to opti現從mize the structure of mRNA and thus科煙 to prolong and control the duratio雨秒n of expression，provided that safety林女 of the vaccine商照 could be safeguarded. The immun話長ological mechanism of mRNA va見如ccine was summarized in the paper 少廠from the aspects of classifi票中cation，action mechanism，techn飛鐘ical advantages and delivery syst唱新em of the vaccine，with a vie金秒w to providing a reference for fut你章ure development of t老這he vaccine.全文下載鏈接：https://k話草ns.cnki.net/kcms/d秒草etail/detail.aspx?南歌dbcode=CJFD&dbname=CJFDAUTO&filename=Z媽年GDW202205015&uniplatfo員對rm=NZKPT&v=vPzqR5W2eDC7Mw5fpeqWvOQ21WXA聽行_xM3hyjFT3cV01RJxJ玩通d7zHADlxQdbPazMzqv
2022-06-07
禽星狀病毒研究進(jìn)展
禽星狀病毒（avian astroviru能和s，AAstV）屬于星狀病毒科，包含多種(zhǒng)亞唱務型，可以引發(fā)禽腎炎、鴨肝炎以及火雞腹瀉等多種(zhǒng)疾病，禽類感生在染後(hòu)表現生長(cháng)發(fā)鐵家育遲緩、産蛋下降，嚴重時(shí)可導緻死亡，給養殖業造成(chéng)一定的身船損失。此外，AAstV具有感染人去數的潛在風險。目前AAstV的緻病機理暫不清風日楚，隻有火雞星狀病毒引發(fā)腸炎的緻病機制可作爲參考。許多方我輛法已用于AAstV檢測，如電鏡檢測、酶聯免疫吸附試驗、細胞培養服謝和病毒分離以及分子技術檢測等。AAstV亞型較多，疫苗和藥物研發(fā)存遠睡在較大難度，給該病防治帶來一定的挑戰。本文圍繞AAstV的病原學(x自來ué)、國(guó)内外流行情況、感染後(hò低志u)臨床症狀及緻病機制以及檢測方法和控制藍嗎措施等進(jìn)行了綜述，以期爲後(hòu)期進(jì車行n)一步研究提供參考。Advances in t不風he Researches on A北但vian AstrovirusAvian 知都astrovirus（AAstV），a member of astrovir舞體idae family，includes輛習 a variety of subty弟也pes，which may le又通ad to avian nephritis，duck hepati麗土tis，turkey diarr靜明hea and other diseases. Such symptoms動房 as growth retardation and decline火東 in egg production e樹通ven death may be observed in infe們兒cted poultry，which wou姐制ld bring certain losses to兒公 breeding industry. In厭司 addition，the virus may bring poten志紅tial risk to human. Howe那讀ver，its pathogenic mechanism has been劇森 unknown，except the one for 雜數enteritis caused by Turk事小ey astrovirus t煙綠hat may be used as a refe筆習rence. The virus may be detected by ma請視ny methods，such as ele購拍ctron microscopy，enzy通河me-linked immunosorbent ass關男ay（ELISA），cell cult章費ure and virus iso如校lation，molecula術話r technology，etc. However，it would be習頻 difficult to develop vacci得體nes or drugs due to many subtypes，w拍間hich brings certain 電謝challenges to the弟科 prevention and treatmen站紅t of the disease. In the近視 paper，the etiology，epidemic st鐘算atus all over the world，clinical sym相鐵ptoms，pathogenic mechani窗還sm，detection methods an去劇d countermeasures were summ照小arized，so as to provide a reference f房得or further researc日多hes in the futur外但e.全文下載鏈接：https://kns.c雨校nki.net/kcms/detail紅水/detail.aspx?dbcode=CJFD&d開火bname=CJFDAUTO&filename習校=ZGDW202205014&uni姐技platform=NZKPT&朋妹v=vPzqR5W2eDBeCnQWiQE2jP2g7DOF7Tnk4Ur的拍oGDd-1gWqfpREXbWVt_DcASTXQcb裡費Y
2022-06-07
泰萬菌素對(duì)豬繁殖與呼吸綜合征的防短舞控效果試驗
爲檢驗泰萬菌素對(duì)豬繁殖與呼吸綜合征（PRRS）藍還的防控效果，用2種(zhǒng)泰萬菌素制品（A、媽友B）和黃芪多糖飼喂妊娠母豬，通過(校相guò)統計臨床生産指标、母豬和仔豬抗體水平、病毒的高檢出率以及細胞因子IL-2、IFN-γ表達水平等，綜合評價泰萬菌素的PRRS雪對防控效果。結果顯示：泰萬菌素的歌行PRRS防控效果明顯優于黃芪多糖，其中泰也草萬菌素A、B組母豬的産健仔率由試驗前的84.也山98%分别提高到94.44%、91.89%，産弱胎、好妹死胎或木乃伊胎率相應降低；泰萬菌素A、B組母豬流公為産率降低，體内抗體下降速率減緩，就少所産仔豬抗體穩定性和整齊度提高，母豬血液中的PRRS多劇病原檢出率和濃度降低，IL-2表達水平下降，IFN化用-γ表達水平上升。結果表明，泰萬菌素可降低妊娠母豬因PRR村問S造成(chéng)的繁殖障礙率，同時(shí男做)可通過(guò)調控細胞因子水平增強機體免疫力，從而起(qǐ看日)到防控PRRS的作用。本研究爲PRRS防控提供了一種(zhǒng)新思路。E熱家valuation of Prev就員ention and Control Ef現拍fect of Tylvalosin on PR草哥RSIn order to evaluate the pr畫去evention and control effect of tylvalo視我sin on porcine reproducti呢樹ve and respiratory s亮在yndrome（PRRS），pr就西egnant sows were fed with two ki白姐nds of tylvalosin produc服音ts（A and B）and as日時tragalus polysaccharide to co暗愛mprehensively evaluate信科 the effect of tylv在海alosin by means of cli吧線nical production indicators，antibod什一y levels in sows and piglets，viru我也s detection rate關動 and expression level of cyto子做kines IL-2 and IFN-γ. The results sh理物owed that，compared to astragalus p筆訊olysaccharide，tylvalosin was mo你討re effective for時市 PRRS，for groups tylv銀討alosin A and B，the healthy litte中南r rates of sows were in和近creased from 84低快.98%（prior to evaluation）to舊林 94.44% and 91.89%，respectively，and the紙靜 rates of weak fetus，stillb吧北irth or mummified fetus decreased accor區快dingly；the abortion rate身友 decreased，the decline rate o姐門f antibody level in vivo slowed down，公自the stability and uniformity of an秒銀tibody level in their piglets increa事黑sed，the detection rate a外通nd concentration of PRR都會S pathogens in sows' blood通數 decreased，the expression leve計去l of IL-2 decreased，and that of綠什 IFN-γ increased. In con我會clusion，tylvalosin could reduce 數街the reproductive disorde河月r caused by PRRS i有雨n pregnant sows，enhance immun河音ity by regulating the lev業機el of cytokines，and consequently prev也化ent and control PRRS. A new idea近離 was thereby provided by the study fo國雨r PRRS prevention and control. 全文下白筆載鏈接：https://kns.c文物nki.net/kcms/detail/detail.asp山玩x?dbcode=CJFD&dbname=CJFD也子AUTO&filename=ZG吧嗎DW202204014&uniplat西和form=NZKPT&v=vPzqR5W2eDCvfIyFR中媽9ITE4EXTmGwX9N6aCUok行綠DUO8EEmm-LgXq_LsMPU8IKVP5Mv
2022-04-20
戊型肝炎研究進(jìn)展
戊型肝炎（hepatitis E，HE）是由戊型肝炎病毒（he火藍patitis E virus，HE歌裡V）引起(qǐ)的一種(zhǒng)病毒性人獸共患傳染病來還。自1997年在美國(guó)首次子都從家豬體内發(fā)現HEV以來，已确定該病毒能(néng)夠感染人類以場書及豬、鹿、兔、駱駝、大鼠等多種(zhǒng)動物。HEV通常會(huì)作暗導緻肝衰竭、慢性肝炎以及肝外神經(jīng)和腎髒疾病。該病主對訊要通過(guò)糞口途徑傳播，也可以通拿熱過(guò)血液傳播。據估計，全球每年約有2 000萬人感染HEV，年她紅輕人病死率爲0.5%~3.0%，而孕婦感染後(hòu)死理費亡率可高達30%，因此HEV對(duì)公共衛生安生秒全構成(chéng)了巨大的威脅。本文主要對(duì)HEV的病原學(報黃xué)、流行病學(xué)、診斷、防控等方面(miàn)的研她市究情況進(jìn)行綜述，旨在進(jìn)一步提高相門少關從業人員等對(duì)本病的認識，以便做好(hǎo)研究和防控應對在相(duì)工作。Introduction to the Studies o和雜n Hepatitis EHepati視訊tis E（HE）is a viral zoonosis照答 caused by infection 南校with hepatitis E vi聽南rus（HEV）. Since 1997，when HEV was fi愛中rstly detected from domestic pigs in t火姐he United States，it has中北 been confirmed that human，pigs謝畫，deer，rabbits，camels，rats and 下算other animals could be infected wi山去th the virus. HEV us車麗ually leads to liver failure，ch市下ronic hepatitis and extrahepati弟他c nerve and kidney diseases. T樂那he disease is mainly transm南子itted through fecal-oral route in ad藍門dition to blood. It is 短上estimated that about 20 mill亮話ion people are in林雪fected with HEV every year村短 in the world，with a mortalit你遠y of 0.5% to 3.0% in young people，北書and up to 30% in pregnant women with 鄉靜infection，posing a great threat to 體放public health safety. I黑朋n the paper，the studies on 一黃the etiology，epidemiology，diagno國開sis，prevention and control of HEV 請輛were summarized街身，with a view to further improving th慢風e awareness of r笑廠elevant practitioners to m坐銀ake them better research 紅民or respond to future outbreak. 廠商 全文下載鏈接：https://kns.cnki.net/kcms/det嗎媽ail/detail.aspx?dbcode=CJFD&d鄉兒bname=CJFDAUTO&filena劇熱me=ZGDW202204013&uniplatfo關煙rm=NZKPT&v=vPzqR5W2e分制DCdCWwoQEPMqGCoD7M3B4PtQ2ac0好說IW9ZIXAgMK-D6QHX_3uApSruhRE
2022-04-20
弓形蟲實驗室診斷技術研究進(jìn)展
弓形蟲病（toxoplasmosis）是由剛地弓形蟲（Toxoplas國有ma gondii）感染引起(qǐ)的一種(zhǒng)人獸共患寄生蟲病低很，在世界各地都(dōu)有分布，宿主種(zhǒ雜熱ng)類十分廣泛，人間和動物的感染率都自風(dōu)比較高，對(duì)人類健康和答得公共衛生構成(chéng)了威年朋脅。目前，弓形蟲病沒(méi)有理想的藥物用于治療，早期空大診斷是防控該病的重要手段之一。病原學(xué)診了請斷是最早建立的弓形蟲診斷方法，操作簡單，結果可靠，但耗時(shí)較問校長(cháng)，無法滿足快速、高電報效、批量檢測的需求，因此在實驗室診斷方化但面(miàn)應用逐步減少。免疫學(xué)檢測方法以ELISA紙工和IHA方法爲主，已有較爲成(chéng)熟的商品話樹化試劑盒或診斷試劑，在大規模的檢測船綠和流行病學(xué)調查中起(通海qǐ)到了很大作用。分子生物學(xué)方法與白刀傳統的病原學(xué)檢測方法相比，們鐵其速度、通量、特異性、敏感性各方面(miàn)都(dōu)有提升，相較于人朋免疫學(xué)診斷，避免了獲得性免疫帶來的假陽性結果，是未來弓形蟲拍行病實驗室診斷的主要發(fā)展趨勢。本文從病原學(xué)、分長開子生物學(xué)和免疫學(xué)方面(miàn)對(舞線duì)弓形蟲的實驗室診斷技術進(jìn)行論述，愛她以期爲弓形蟲的實驗室診斷和防控措施的制定提供參考。Advances in 門店Laboratory Diagno離還stic Techniques f拍技or Toxoplasma gondiiTox少他oplasmosis is a zoonosis caused朋玩 by infection with Tox吃視oplasma gondii，and widely distri司討buted all over the world，w唱藍ith wide range of host species國靜 and high infection rates in hum理機an and animals，posing a麗通 threat to human health an朋年d public health志城. Considering that no ideal 國草drug has been available for the di人長sease，early diagnosis is one of the 坐科important means to prev西他ent and control the disease. Etiolog好技ical diagnosis 拍裡is the initial method木放 for Toxoplasma g如地ondii，and is simple with reliable resul子飛ts but time-con下做suming，which failed to meet the nee玩道ds of rapid，efficient and訊輛 batch detection，and be機慢came decreasing in its appli和還cation in labor事術atory diagnosis. Immunol內放ogical detection methods mainl朋愛y include ELISA and IHA，and relativel關媽y mature commercia人市l kits or diagno公上stic reagents are ava短個ilable，which have played a gre路不at role in large-scale de拍了tection and epidem件國iological inves河國tigation. Compar員紙ed with traditional pathogen可路ic detection methods，molecular biologic區公al methods have村木 been improved in speed跳好，throughput，specifi微答city and sensitivi聽刀ty，and avoided false positive results c日分aused by acquired immuni草資ty as compared with 呢個immunological diagno都鐘sis，which represent the main devel這司opment trend of labora嗎雪tory diagnosis for toxoplasmosis in問熱 the future. In空劇 the paper，the laboratory 站訊diagnosis technology for Toxoplasma窗森 gondii were discussed from the aspe道拍cts of etiology，molecular biology an員機d immunology，with a v訊姐iew to providing a reference光唱 for the laboratory diagnosis for Toxop光站lasma gondii and developme麗物nt of measures fighting a得很gainst toxoplasmosis.全文下載鏈接：https算鐘://kns.cnki.net/kcms/detail不高/detail.aspx?dbcode從得=CJFD&dbname=CJFDAUTO&filename=ZGDW2森路02204012&uniplatform=NZK商草PT&v=vPzqR5W2eDDvN3i09AaxeTq4從兵bSAnb50ZtBYX-R2auVZdzGB80AQs6o章什Mt30Dkv88v
2022-04-20
豬源多殺性巴氏杆菌的分離鑒定及其主要抗原蛋白的原核表達能子
2021年3月，山東省龍口市某養豬場發(fā)生了一起(qǐ)以敗血症爲輛吧主要臨床症狀的烈性傳染病。爲對(duì)引起(qǐ)此窗如次疫病的病原進(jìn)行确診間雨，無菌采集發(fā)病死亡仔豬的肝髒、脾髒等髒器進(jìn去什)行染色鏡檢、病原分離培養、生化鑒定道照及PCR檢測。結果顯示，本次分離株的形态結關街構、培養特性及生化結果與多殺性巴氏杆菌較爲一緻，山快且可特異性擴增出多殺性巴氏杆菌特異性基因Kmt，影道因此本次分離菌株爲一株豬源多殺性巴氏杆菌，命名爲LK01。随後玩風(hòu)，對(duì)該分離株的5個主要抗原蛋白基因（OmpH、Om視舞pA、AspA、pIpE和TAA）進(jìn)行克隆與原核表達。先將(jiān媽子g)PCR成(chéng)功擴增出的目的片段與雙酶切回收後(hòu)的pCo場開ld-I載體進(jìn)行同源重組連接，測序結果正确的重組質粒轉化至大腸杆菌身城BL21（DE3）感受态細胞中，随後(hòu)經(jīng)IPTG誘導後(h民化òu)進(jìn)行Western blot什訊檢測。結果顯示，在30.0、21.5、52.0、37.5多和和45.5 kDa處出現特異性醫喝條帶，表明各重組蛋白均成(chéng)功表達。本研究爲開(kā音站i)展豬源多殺性巴氏杆菌病的流行病學(xué)研究能票，開(kāi)發(fā)新型重組疫電匠苗與診斷試劑奠定了基礎。Isolation and Ide習說ntification of Porcine Pasteurella m了的ultocida Strain and Pro笑城karyotic Expression of It他上s Major Antigen ProteinsIn March生地 2021，a severe infectious disease wit雜和h major symptom of septicemia occ員器urred in a swine分現 farm in Longkou City，Shand樹熱ong Province. In order to identify the妹腦 pathogen leading t短秒o the disease，livers 山鐵and spleens of dead pigl器哥ets were aseptically c些靜ollected for staining microscopic e裡兒xamination，bacterial is城還olation and culture們上，biochemical identification and 時購PCR detection. The r慢去esults showed tha時我t the morphological struct熱件ure，culture characteristic文能s and the results of biochemical tes頻北t of the isolate were relativ明金ely consistent with those of Pasteurel女但la multocida（PM），then Kmt，the spec微物ific gene of PM was succ業山essfully amplified，it was ident還作ified that the is腦內olate was a porcine PM named as L著東K01. Subsequently，its five major ant鐵章igen genes（including OmpH，Om放公pA，AspA，pIpE and TAA）were cloned and ex是司pressed in prokaryotic 呢木expression system. By homologous r老自ecombination，the target fragments海答 amplified by PCR were linked with t錢外he pCold-I that was化下 after double digestion. The recombi服匠nant plasmid with correct seque市公ncing results was transformed into E. c醫少oli BL21（DE3）cell很下s and induced by IPTG for Wes日就tern blot detectio飛村n. It was observed that specific band制匠s appeared at 30.0，21.5，52.0，37.5 and的雪 45.5 kDa，indicating that all recombina市很nt proteins were successfully低看 expressed. A foundation was 計裡thus provided for further epidemiolo機子gical study on po睡吧rcine PM and for development時是 of new recombina見店nt vaccines and diagnostic reagent煙是s.全文下載鏈接：https://kns.河科cnki.net/kcms/detail/de玩家tail.aspx?dbcode=CJFD&dbname草村=CJFDAUTO&filename=ZGDW2022匠做04007&uniplatfor請為m=NZKPT&v=vPzqR5W2eDC80-MzvwQzQfqa妹志ORuBw6l2HfPDjPUNminpICQvcKvjTO4黑花pmkroMcNw
2022-04-20
4株雞源多殺性巴氏杆菌生物學(xué)特性分析
禽多殺性巴氏杆菌可引起(qǐ)禽出血性敗血症，又稱爲禽巴氏杆菌病、禽霍亂，發什間(fā)病快，死亡率高。爲對(duì)禽多殺公線性巴氏杆菌的緻病機制研究及臨床防治提供支持，相相通過(guò)PCR鑒定、耐藥性分析、生物被(b跳一èi)膜形成(chéng)能(務去néng)力試驗以及半數緻死量（LD50）測定，對(duì)4株臨床分離的跳很雞源多殺性巴氏杆菌（Pm01、Pm看件03、1801及1803）進(jìn)行了相關生物學(xué)特性分析。PCR那吃鑒定結果顯示，4株多殺性巴氏杆菌均事也爲莢膜血清A型，均含有pfhA、exBDtonB、nanB、oma87也請、ompH、hgbB、hgbA、sodC、sodA 9種(z匠紅hǒng)毒力基因，不含有toxA、去道nanH、ptfA 3種(zh舞新ǒng)毒力基因；耐藥性分析結果顯示，4株多殺性巴氏杆菌對(du校女ì)克林黴素、紅黴素、四環素、卡那黴素、阿莫西林、頭孢噻吩和氨苄西林見一等7種(zhǒng)抗生素表現出全部還鐵或部分耐藥；生物被(bèi)膜形成(chéng)能(néng喝頻)力試驗結果顯示，Pm01菌株能(néng)形成(chéng)極強的生物被(b秒山èi)膜，而Pm03、1801以及1803菌株幾乎不能(néng)形成場地(chéng)生物被(bèi)膜；LD50測定結果顯示，4菌株LD西區50分别爲1.33×101、2.內這74×102、4.22和4.22 CFU。結果表明，4株雞源多殺性巴好劇氏杆菌含有多種(zhǒng)毒力基因，均具有較強的的飛緻病性，其毒力與生物被(bèi)膜形成(chéng)能(刀知néng)力不完全相關，且對(duì)多種(zhǒng)商區抗生素耐藥。本研究爲禽巴氏杆菌病防治提供謝房了技術支撐與參考數據。Analysis on the Biologi兵藍cal Characteristics of Four Strains 小如of Pasteurella multocida Derived from C近要hickenAvian hemorrhagic討能 septicemia，als為自o known as avian pasteurellosis or舊紅 avian cholera，is caused子從 by Pasteurella multocida，w畫的ith rapid occurrence and h雨都igh mortality. In order 頻銀to support future researches on the pat科關hogenic mechanis唱為m of the bacteria as well as preven現土tion and control of the disease哥有 in practice，relevant biol月冷ogical characteristics 秒靜of four strains歌還 of Pasteurella multocida（Pm01，P飛訊m03，1801 and 1803）were analyzed throu聽為gh PCR identification，drug resis少又tance analysis，biofilm forma藍相tion ability te事技st and median lethal dose（LD50）d廠算etermination. It was showed that，城區by PCR identification，all 和西the four strains were capsular sero鐘開type A and contained 9音冷 virulence genes inc事金luding pfhA，exBDtonB，nan土風B，oma87，ompH，hgbB，hgbA，sodC an厭件d sodA，except t有多oxA，nanH and ptfA；b又校y drug resistance analysis，the four s女很trains were fully or partially resist近亮ant to 7 antibiotics in聽動cluding clindamycin，ery問站thromycin，tetracycline，kanamycin，amoxi遠問cillin，cefotaxime and ampicill去為in；by biofilm formation abil是舞ity test，a very strong biofi街雜lm was formed by Pm01 strain，but fai雨風led by Pm03，1801 and 1803；and b好多y the LD50 determination test土舊，the LD50 value村離s of the four strains were 1.33×101，2森呢.74×102，4.22 an的章d 4.22 CFU，respectively. In conclu鐘子sion，all the four strains contain間照ed a variety of virulence師計 genes that were with strong 姐還pathogenicity，for which the virulence 書高was not completely related to t子路he biofilm formation ability，and 家慢resistant to a 好北variety of antibiotics. A刀學 technical support and referenc和子e data were provided for future pr拿你evention and contro還開l of avian paste要區urellosis.全文下載鏈接：https://kns.cnki務少.net/kcms/detail/d自開etail.aspx?dbcode=CJFD&dbname=CJFDAUT在裡O&filename=ZGDW202204022&uniplatfo我身rm=NZKPT&v=vPzqR5W2eDDek5QoglmXmOjNANS微小1XBh7FovRVaOHHwT9187EAklnIYM工聽-4ITWcDa6
2022-04-20
新疆和田鵝源沙門氏菌血清型鑒定及耐藥性分析
爲了解新疆和田地區鵝源沙門氏菌流行血清型及其耐藥性，無菌采集和田地區兩(l河開iǎng)個鵝場（A場和B場）病死鵝内髒、肛拭子樣(yàng)高厭本，用常規方法進(jìn)行沙門氏菌分離鑒定個了，利用PCR進(jìn)行驗證和血清型老低鑒定，用K-B法進(jìn)行分離株子票耐藥性檢測。結果顯示：共分離出14株低黑沙門氏菌，總分離率爲1.81%（14/773）輛房。A場死亡鵝内髒樣(yàng)本的沙門氏菌分離率爲1.33刀拿%（4/300），肛拭子樣(yàng)本分離率爲1.66%（5動物/300），在存活病鵝肛拭子中未分離出沙門舊報氏菌（0/25）；B場死亡鵝内髒樣(yàng)作東本的沙門氏菌分離率爲20.00%（4/20），肛拭子樣(yàn都拿g)本中未分離到沙門氏菌（0/19），存活病鵝肛拭子樣(yàng)腦下本分離率爲0.91%（1/109）。共分離出4種(z老跳hǒng)血清型，占比分别爲鼠傷寒沙門氏菌42.86%（6/14）、腸炎沙門氏空姐菌21.43%（3/14）、德比沙門氏菌7.14%（1服我/14）、紐蘭沙門氏菌7.14%（1/14）。沙門氏菌分離株對(d身大uì)多黏菌素B、哌拉西林-他唑巴坦、阿莫西林-棒酸和頭火黑孢吡肟較爲敏感，對(duì)其餘10種(zhǒng)抗菌藥物具有一定耐藥性，討短其中對(duì)氨苄西林和鏈黴素耐藥資車性極強。結果表明，和田地區鵝源沙門氏菌血清型複雜，其中鼠傷寒沙門氏菌爲優勢資也血清型，不同養殖場流行的血清型種(zhǒng)類及優勢血清型多冷有差異；流行的鵝源沙門氏菌耐藥呢學較爲普遍，且表現爲多重耐藥。結果提示，各養殖場應根據流行的具體血清型沙門氏菌森商株，科學(xué)合理使用臨床抗菌藥物。Serotyp地兵e Identification and Drug Re快跳sistance Analysis of Salmonella of G一知oose Origin in Hotan，XinjiangIn order t明文o identify the p歌司revalent serotypes會綠 and drug resistance of Salmone開農lla of goose origin i路理n Hotan，Xinjiang，v身公isceral and anal男說 swabs of dead g費白eese and anal swabs of sick志費 geese were aseptically collected 區會from two farms（Farm A and Fa聽得rm B）in Hotan for isolation and ident吃們ification of Salmonella by convention嗎志al methods，followed b道個y validation and serotype iden跳購tification by PCR，then drug resistance物數 analysis of the isolate少西s by Kirby-Bauer disk diffu購金sion susceptibility test（KB test）但火. The results showed 輛好that 14 strains of Salmonel市土la were isolated，with a total isolat有術ion rate of 1.81%（14/773）. Fo上可r Farm A，the iso文短lation rate of visceral samples o風藍f dead geese was 1.33%（4/300女門），and that of anal s離分wab samples was 1.66%（5/300生制），but no Salmonella was is制秒olated from anal swa訊中bs of sick geese（0/25）. For Farm B，可報the isolation rate of 光慢Salmonella from visceral sa購謝mples of dead geese was 2時讀0.00%（4/20），none was isolated fr是音om anal swab samples（0/1公兵9），and that of a校朋nal swab samples of長報 sick geese was 0.91%（1/109）. Four ser子吧otypes were isolated少長，including Salmonella typhimurium a樹西ccounting for 42.86%（6/14），Sa習靜lmonella enteritis for 21.43%（3/14），朋志Salmonella derby鐘計 for 7.14%（1/14）and Sal工現monella newland for 7.14%（1/14）. The is光子olates were sensitive to polymyxin B，雜微piperacillin tazobactam，amox謝不icillin clavulanic acid and c街路efepime，and res視和istant to other 10 antibioti外上cs to some extent來鐘，especially extremely resistan一音t to ampicillin and strepto妹水mycin. In conclusion，the serotypes of 吧學Salmonella in Hotan were complex，i多聽n which Salmonella typhimurium was報為 the dominant one，and the prevalent and睡討 dominant serotypes were different 通厭depending on differen愛麗t farms；the drug 東雨resistance，especially 們還multiple one，of the pr下信evalent Salmonella 是開strains，was common. It was therefore 請輛recommended that antibiotics should be開校 scientifically and reasonably use放房d in clinical practic務生e according to specific se了場rotypes prevalent說睡 in each farm.全文下載鏈接：https://kns.cnk視一i.net/kcms/detail/detail.asp山件x?dbcode=CJFD&dbname科你=CJFDAUTO&filename=ZGDW20能可2204006&uniplatfo離就rm=NZKPT&v=vPzqR5W2eDCGPtZcByj照到XTb36z7qYTRdIHLaFpZvohiohcBBQL_JS店街6VoguSJjlwDw
2022-04-20
我國(guó)部分地區病死畜禽無害化處喝房理中心病原滅活情況調查
爲了解我國(guó)病死畜禽無害化處理環節病原滅活效果，2020年兒吃對(duì)11個省（自治區）52個病死畜禽無害化處理中心開(kāi)鄉刀展抽樣(yàng)檢測、問卷調查。通過(guò)對(duì)樣(yàng他暗)品進(jìn)行病毒核酸檢測和病毒核酸陽性樣(yàn術中g)品病原活性檢測發(fā)現，化制法、發(fā)酵法、碳化法、酸解法均可畫坐滅活非洲豬瘟、豬瘟、口蹄疫和豬繁殖與呼吸綜合征等4種(zhǒng)少視疫病病原。通過(guò)問卷調查發(fā訊街)現，無害化處理中心因廠區布局、管理制度、操作措施等方面(miàn讀門)問題，存在終端産物被(bèi)書女病原二次污染或病原随産物擴散至場外的風險。因此，要規範無害化處從地理企業建設，加強企業日常監管和無害化處理産物監管，降低病原傳出風險。Inve飛理stigation on th木哥e Pathogen Inactivation by B空為io-safety Disposal Centers in Ch我玩inaIn order to investigate the也湖 effect of pathogen inactivation by 都厭bio-safety disposal centers for習雜 dead livestock and poultry in Chin慢匠a，52 centers in 11 provinces/autonomous吧白 regions were sampled，tes好店ted and investigated by qu笑上estionnaire in 20美妹20. It was found that，thro東相ugh detection of vir校公al nucleic acid a拿錯nd pathogenic activity o又黃f viral nucleic acid positive samp校拿les，African swine feve自黃r virus（ASFV），classical swine fever議通 virus（CSFV），foot-and-mouth disease v章們irus（FMDV）and porcine reproductive 影東and respiratory syndrome玩這 virus（PRRSV）could be inactivated by 妹可rendering，fermentat山爸ion，carbonization and acidic hydrolysi頻資s. Based on the result和制s of questionnaire investigation，冷地due to the problems in科林cluding site layout，mana業信gement system，operation measures他如 and other aspects ap亮歌plied in the centers，there was p如在otential risk of secondary contaminat火西ion in terminal products by pathogen木裡s or spreading pa快風thogens to the premises out事裡side the centers with products.那商 Therefore，it was nec煙男essary to standardize the cons妹木truction of bio-safety鐘議 disposal enterprises，and to streng小下then daily supervisi唱對on for the enterpris為中es and supervision for the products un上紙der bio-safety disposal to reduce p到在otential risk of sprea校作ding any pathogens.全文下載鏈接：https:/東個/kns.cnki.net/kcms/detail/deta作費il.aspx?dbcode=坐如CJFD&dbname=CJFDAUTO&f這的ilename=ZGDW202204005&uniplat可件form=NZKPT&v=vPzq北她R5W2eDA9hDQ3FavBVDxGNgk低街ytV5qGdI1SMOEpserDBq88MVh6tZHhfoFv-Rh
2022-04-20
2019—2021年我國(guó)媽很29個省級行政區雞傳染性支氣管炎流是錢行病學(xué)調查
爲了解雞傳染性支氣管炎病毒（IBV）在我國(guó睡水)的流行規律，通過(guò)RT-PCR方法，對(du林行ì)2019—2021年從我國(guó)29個土些省級行政區送檢的38 442份疑似IBV感染樣(y小車àng)品進(jìn)行病原檢測和鑒定，對(duì但小)檢出的陽性樣(yàng)品進(jì生公n)行S1基因測序分型，然後(hòu)對(duì化村)檢測結果進(jìn)行時(shí)間、空間和群間統計分析。結果顯示廠說：共檢出6 436份IBV陽性樣(yàng)本工林，陽性檢出率爲16.7%。2019—2021年的IBV陽性檢出率照聽分别爲12.5%、16.4%、21.0%，呈逐年增長(cháng件裡)趨勢：陽性檢出率呈現一定的季節性變地河化特點，7—9月檢出率（11.3%~15.4%）較低，而月這3—6月（17.3%~19.3%）和10—12月（17還會.5%~19.0%）較高。全國(g場家uó)29個省級行政區中有27個檢出陽性樣(yàng)品，其中東嗎如部和中部家禽養殖密集區陽性檢出率較高，而西部和北部地區較低。白羽肉雞陽性檢少市出率最高（28.9%），肉種(zhǒng)雞陽不湖性檢出率最低（5.0%），0~6周齡階段IBV靜師感染較爲嚴重。共檢出5種(zh一購ǒng)基因型，其中QX型（66.5%）、GVI型（27.7姐現%）占比較高，爲當前流行的優勢基因型；不同品種(zhǒng)雞群中流行的基會嗎因型存在一定差異，白羽肉雞群中以QX和GVI型爲跳子主，而其他雞群中流行的基因型較爲複雜；QX型0~3周空鐵齡（33.2%）、GVI型3~6周齡（66.4%）陽性檢出數量占比道廠較高，6周齡以後(hòu)占比均較低（＜街做14.0%）。結果表明：我國(guó)雞群中IBV感染普遍，尤靜我其是家禽養殖密集區，且流行呈逐年加重哥家趨勢；冬春或秋冬季節多發(fā)，小日齡雞群感染嚴重；流行基因型複雜，以QX身黃型和GVI型多見。結果提示，我國(guó)的IB防控面(miàn)臨較大哥我壓力與挑戰，需要加強優勢基因型疫苗的研發(fā)媽唱，重點做好(hǎo)小日齡雞群的IB防控。E電又pidemiological Investigatio鄉問n on Avian Infecti是我ous Bronchitis in 29 Pro器公vincial Administ空站rative Regions of China from 2019 議吧to 2021In order to ident市那ify the prevalence rule of a能店vian infectious bronchitis v白說irus（IBV）in China，38 442 samples wi民拿th suspected IBV infection submitt雨長ed by 29 regions at pro頻子vincial levels from 2019 to 2021 wer河朋e detected and identified by RT-P民體CR. The S1 genes of positive samples we關事re sequenced and typed，and then the r懂關esults were statistically anal員件yzed from the aspects做新 of time，space and intergr海森oup. The results sho車西wed that 6 436 IBV positive samples we討對re detected，with the positive det快術ection rate of 16.7%.The positiv黑行e detection rate大商s of IBV for the three years看動 were 12.5%，16.4% a慢黃nd 21.0% respectively，tendi舊議ng to increase year by year：鄉去the positive detection rate changed們員 with seasons to certain extent，which得計 was lower from July to Septem科哥ber（11.3% to 15.4%），飛鄉and higher from March to J我雪une（17.3% to 19笑銀.3%）and October to December（1冷謝7.5% to 19.0%）. Posit湖少ive samples were detect文下ed from 27 out of 29 regions，the很他 detection rates 身員were higher in intensive 錯遠poultry areas in the 房都eastern and central regions區跳，and lower in the western and睡風 northern regions. The detectio河聽n rate of white feather broi男票lers was highes廠劇t（28.9%），and that of輛高 broiler breeders was lowest（5.0輛劇%）. The infection with IBV窗道 was more serious in chicke影器ns at the age of 志樹0~6 weeks. Five 購生genotypes were detect用煙ed，especially QX（66.5%）and GVI（27.7%）家森that were dominant genotypes；計們the prevalent genotypes were different訊務 in different breeds，QX and GVI were雨請 the main genotypes in whi謝事te feather broiler flo慢微cks，while the preval子那ent genotypes were comple用相x in other flocks；the po妹火sitive rates of QX and GVI were higher年湖 in the flocks a很短t 0 to 3 weeks old（33.2%）a術地nd 3 to 6 weeks old（66.4醫低%），and lower in t慢關hose at more than 6 weeks old（＜看子14.0%）. In conc姐東lusion，IBV was widely distributed in ch個舞icken flocks in C照員hina，especially in intensive ar那亮eas where the preva鄉煙lence tended to increase year by ye吧黃ar；it occurred frequently i哥師n winter，spring or autumn，and爸都 was serious in the flocks道森 at young day old；the pr有南evalent genotypes were complex，es海那pecially QX and GVI時低 were common. In conclusion，there w兵家as a huge pressure and cha不件llenge to prevent and control IB 費讀in China，thus it was ne鄉窗cessary to strengthen the develo鐵綠pment of vaccine醫兒s of dominant virus genotypes，a區匠nd a priority s民到hould be given to IB control of chic湖草ken flocks at young day old.全文吃喝下載鏈接：https://kns坐讀.cnki.net/kcms/detail/爸子detail.aspx?dbc志多ode=CJFD&dbname=CJFDAUTO&filenam短新e=ZGDW202204020要計&uniplatform=NZKPT&v=vPzqR見飛5W2eDDGd3nEvemc-sUjGnikySnGZY31xk2TX3E匠科m9TINuFTei5CTHVW7jzwJ
2022-04-20
PRRS嵌合病毒活疫苗（PC株）和滅活疫苗聯合免疫母豬應用試驗
爲驗證豬繁殖與呼吸綜合征（PRRS）嵌合兒女病毒活疫苗（PC株）和滅活疫苗聯合應用于母豬的免疫效果，在天津市某P就舞RRS陽性豬場，對(duì)部分母豬實施了兩(liǎng)種(z一的hǒng)疫苗聯合免疫試驗，利用R問相T-PCR和間接ELISA方法進(頻討jìn)行病毒和免疫抗體檢測。結果顯示：接種(zhǒng志遠)疫苗後(hòu)，試驗母豬精神、食欲和體溫均正常，豬肛拭子和飼養環境樣(yà廠冷ng)品中均未檢測到病毒；免疫後(hòu)14~70 坐市d，試驗母豬PRRS抗體陽性率均爲100什著%；免疫後(hòu)42 d，試驗母見外豬的抗體IRPC平均值仍維持在較高水森內平，且免疫後(hòu)14 d，2個試驗組變異系數分别下降31和44個百分章訊點；試驗母豬所産仔豬的均勻度、活潑程度均高于對(duì)照舞日組。結果表明，PRRS嵌合病毒活疫苗（PC株）和滅活苗聯合暗黃免疫安全有效，不僅可以很好(hǎo)地誘導機但刀體産生正常的免疫反應，而且還(hái)可以提高豬群抗上西體的整齊度且無排毒風險，免疫保護期較長(cháng)。人科本研究爲陽性豬場的PRRS防控提供了有效免疫方案。App見離lication Test of Combin很房ed Vaccination of裡為 Live Chimeric Viru理能s Vaccine（PRRSV PC Strain）and 報動Inactivated Vaccine in SowsIn ord新師er to verify the i兵風mmune effect of combined vaccina人草tion of chimeric virus vaccine（PC str新有ain）and inactivated vaccin黑吧e of porcine reproductive and respi看為ratory syndrome（PRRS）applied in藍黑 sows，some sows in a PRRS positive fa媽術rm in Tianjin City were你見 simultaneously va如請ccinated with the tw會從o kinds of vacci海鄉nes，and related samples were collect少票ed and detected for virus nucleic a讀為cids and antibodies by RT-PCR 呢路and indirect ELISA，respectively.就讀 The results showed t時唱hat the experimental廠身 sows were with no對舊rmal spirit，app高拿etite and body tem慢作perature，and no virus was de金腦tected in their sam去報ples of anal swabs and feeding env不務ironment；the positive rate was 100%短海 in 14 to 70 days after va土長ccination；the average IRPC of anti看對bodies remained at a relatively hig低志h level in 42 days after v資喝accination，and the variation從錯 coefficient of the two exper對了imental groups decreased by 31% and 44家算% respectively in 14 月多days after vacci嗎線nation；the evenness and liveliness o拿森f piglets produced by the experi商用mental sows were higher than t姐亮hose by control group. In con舞信clusion，combined vaccination of算匠 chimeric virus vaccine（PC strain）and i這開nactivated vaccine of PRRS was sa舊那fe and effective相議，which not only induced normal 低訊immune response in the bod現些y，but also improved 湖雜the uniformity of antibodies i不身n pigs without any risk of deto購就xification，and provided a long村外 immune protection peri做藍od. An alternative and ef通師fective choice for PRRS prevent聽鐘ion and control was 數但thereby provided for PRRS-positi服體ve farms. 全文下載鏈接：htt這們ps://kns.cnki.net/kcms/detail/detail.a新器spx?dbcode=CJFD&dbname=C樹們JFDAUTO&filename=志視ZGDW202112020&uniplatf水聽orm=NZKPT&v=2vpJqQNi66Gty71SN-LVre9WQUk友湖bzyJK5jmc8ScN4F2kIS-畫小3eCAgsmu3ksgfgayl
2021-12-23
小反刍獸疫病毒芯片式數字PCR檢測方法的建立及應用
爲建立一種(zhǒng)準确、快速、敏感性更高的小反刍獸疫病毒（pe高吃ste des petits rumi資上nants virus，PPRV）檢測但雪方法，建立了一種(zhǒng)芯片生化式數字PCR（cdPCR）檢測方法間河。根據GenBank中公布的PPRV Nigeria75 /1疫苗株N吧見基因序列設計1對(duì)引物，說舞PCR擴增大小爲166 bp片生草段，構建pMDTM18-N标準質粒并優化反應條件，建立了PPRV 下習N基因cdPCR檢測方法，并與實時(s拿員hí)熒光定量 PCR（RT-qP河飛CR）檢測方法的靈敏性、重複性、特異性和臨床樣(yàng)品檢測做了比較分析訊上。結果顯示：當質粒标準品濃度在1.22×（105~10鄉算2）copies/μL範圍時(shí答業)，cdPCR檢測方法比普通RT-PC校廠R靈敏度高1 000倍，且穩定性好(hǎo)，特異性強；當弟器标準品濃度在1.22×（105~10-3）copies/μL範圍時(shí)，上湖cdPCR與RT-qPCR相比具有相同的特異性，但靈敏性農從比RT-qPCR高100倍；與RT-qPCR 公區測定結果（13.29±6.74）copies/μL相比，cdPCR的最低檢測限去分更低，約爲（0.44±0.14）cop愛如ies/μL；cdPCR對(du爸舊ì)47份臨床樣(yàng)本的PPRV核酸陽性檢出率（25.5%）高于R校秒T-qPCR（17.0%）。結果表明，建立的c樹工dPCR方法特異性強、靈敏度高、重複性好(hǎ車日o)，爲預防PPR早期流行提供了一多行種(zhǒng)快速有效的診斷學是和定量檢測方法。Development and Applicatio校友n of a Chip Digital PCR 亮通Assay for PPRVIn order to establish a麗上n accurate、rapid and sensitive method t妹歌o detect peste des petits r筆答uminants virus（PPRV），a chip digit影相al PCR（cdPCR）assay was家家 developed. A pair of primers was desi舊著gned based on the N gene sequence of P信秒PRV Nigeria75/1 vaccine str文唱ain registered in Gen北我Bank. The fragment with th生服e size of 166 bp was a會請mplified by PCR to cons做劇truct pMDTM18-N st少分andard plasmid，followed by optimization動筆 of reaction conditions，the時花n a cdPCR assay for PPRV N gene was est計那ablished，its sensitivity，repeat弟亮ability，specific器上ity and clinical sample detection 少算capacity were compared and花林 analyzed with those of RT-q知來PCR. The results 票去showed that cdPCR was with good st不家ability and spec高文ificity，and its sensi場水tivity was 1 000 times highe子到r than that of RT-PC通鐵R when the concentration of plasm個木id standard ranged 1.務微22×（105~102）copi老看es/μL；cdPCR was with t計件he same specificity a拍服s RT-qPCR，but its sensit少從ivity was 100 times hi些湖gher than that of RT-qPCR when the 開站concentration was 1.22費南×（105~10-3）copie下時s/μL；the lowest detection線器 limit of cdPCR（0.44±歌微0.14）copies/μL wa弟相s lower compared t和師o that of RT-qPC很友R（13.29±6.74）copies/μL；and fo暗拿r 47 clinical samples，the de信就tection rate of PPRV nucleic acid by cd吃員PCR（25.5%）was higher than tha吃煙t by RT-qPCR（17.0%）. In c城請onclusion，the established cdPCR wa白什s with good specifici但內ty，sensitivity and r吧件epeatability，which could sup玩秒port to prevent any early preval也拍ence of PPRV as a rapid光刀 and effective diagnostic and quantitat化飛ive method.全文下載鏈接：https://kns.cnki.net/慢紙kcms/detail/detail.aspx?dbcode=CJ票土FD&dbname=CJFDAUTO&file會動name=ZGDW20211201家船8&uniplatform=NZKPT&v=2vpJqQNi66Hs生還x9rFNbOw9lXSp6DdAn_4kxzLwL3L4Iwsw1LBwHw服房JUAFQCpEGvGIW
2021-12-23
天然免疫RIG-I樣(yàng對爸)受體LGP2在抗病毒免疫中的作用
RIG-I樣(yàng)受體（RL文哥Rs）是細胞質中重要的模式識别受體（PRRs），LGP2是其重線行要的家族成(chéng)員之一。LGP2在農習抗病毒天然免疫應答中具有特殊功能(néng)，能(néng)高拍夠雙向(xiàng)調控RIG-I、MDA5介美年導的I型幹擾素（IFNs）信号通路。在得鐵不同病毒感染宿主的過(guò)程中，LGP2也表現出明顯的功能(nén錢老g)差異。在雙鏈DNA病毒中，LGP2在RIG-I介導的信城購号傳導過(guò)程中起(qǐ)正向(xiàng)調節作用；在單時兵鏈DNA病毒中，LGP2能(né數事ng)夠促進(jìn)CARDs區失活的RIG-I與非典型泛素鏈結合，要綠從而誘導I型IFNs産生；在雙鏈RN空謝A病毒中，正常表達可增強RIG-I、MDA5對(duì黃南)dsRNA的識别能(néng)力；在正義單鏈RNA病毒中，LG都現P2參與對(duì)正義單鏈RNA病毒的識别過(guò)程；在負雪離義單鏈RNA病毒中，LGP2有時(shí)可匠玩作爲IFNs的誘導劑。另外，LGP2在适應性免疫頻能應答中也發(fā)揮著(zhe)重要作用，能(néng)夠通過(g資輛uò)不同途徑調控T細胞存活與凋亡。目前在調慢司控RIG-I、MDA5介導的信場動号通路與抗病毒免疫應答中，尚不清楚LGP謝議2發(fā)揮的确切功能(néng)和其作用機制，未來可進(jìn)一步風近加深LGP2在細胞免疫應答中的作用及機制研究。本文就(jiù)近年來LGP笑村2在RLRs介導的信号通路和抗病毒免疫應答化些中發(fā)揮的作用作綜述，以期爲LGP2的進(jìn)一步研究和機體議了抵禦病毒感染新機制提供參考。Studies on低購 the Roles of Innat少黃e Immune RIG-I-like Receptor LGP化道2 in Antiviral ImmunityRIG-I-like rec月音eptors（RLRs）are the key pattern recogni兒在tion receptors（PRRs）in cytoplasm，and能好 LGP2 is one of its important 呢匠family members. L讀市GP2 functions specially in antiviral i如能nnate immune response，a月很nd could regulate the 村刀type-I interferon（IFNs）signaling mediat吃匠ed by RIG-I and MDA5 bidirec房理tional. Functional民內 differences are als報湖o obviously present in LGP2 村師during the process of infection with日舞 different viruses in host學金s.LGP2 positively regulates the progre跳鐘ss of signaling mediated 微報by RIG-I in double-s書開tranded DNA（dsDNA）v快雪iruses，induces type-自到I IFNs through supporting水金 the binding of ina腦筆ctivated RIG-I loca都店ted in CARDs region with atypi人南cal ubiquitin cha民家inin single-stranded DNA（ss短好DNA）viruses，increases知靜 the capacity of RIG-I and MDA議輛5 to recognize dsRNA through 村雪normal expression in double-stranded站什 RNA（dsRNA）viruses，participa體但tes in the process of reco請雜gnition for positive-sense si為計ngle-stranded RNA（+ssRNA）viruses河票，and is sometimes u購懂sed as an inducer for IFNs 議對in negative-sense single-stranded RNA（家關-ssRNA）viruses. In additi報兵on，LGP2 also plays an個校 important role i樂亮n adaptive immune response，regulati樹公ng the survival and apoptos月用is of T cells through differe就雜nt ways. Currently，its specif道離ic functions and麗算 mechanisms for reg年事ulation of signaling mediated by雨哥 RIG-I and MDA5 and for但山 antiviral immune response are一司 still unclear，which should be further下近 studied in the future. In the paper，明間the studies on the低如 roles of LGP2 in signaling med技機iated by RLRs and antiviral immun答那e response in recent years were r視個eviewed，with a view事人 to providing a reference for f知睡urther study on LGP2 and new mechani上男sms of resistance to viral infecti呢錢on.全文下載鏈接：https://kns.cn地唱ki.net/kcms/detail/detail.aspx?dbc近人ode=CJFD&dbname=CJFDAUTO&化上filename=ZGDW202112017&uniplatform=NZ車機KPT&v=2vpJqQNi66ErScKeXzQGj8VwgZo-gA4美工TnGAGyZxXqhRCxps7PW9rE1W-DEjpWR不謝zf
2021-12-23
全球基因I型非洲豬瘟病毒流行與疫苗研究進(jìn)展
非洲豬瘟病毒已在我國(guó)定殖并形成(chéng)較大污熱個染面(miàn)，國(guó)内樣(yàng)品中發(fā)現基因I型非洲聽行豬瘟病毒，提示當前臨床中實際流行的病毒種(zh紙銀ǒng)群更加複雜。基因I型毒株從19視森57年傳入葡萄牙後(hòu)，研究人員就(jiù)開(kāi)始對(duì)其鐵高進(jìn)行研究。多年來，國(guó)外對(duì)基因I型毒株車微的流行分布情況特别是弱毒疫苗進(j司靜ìn)行了大量研究，但國(guó)内目前尚無這(聽熱zhè)方面(miàn)的分析讨論。爲此，作者就(jiù)基因I型喝們非洲豬瘟病毒流行與疫苗研究現狀進(jìn)行綜述，以期爲行區我國(guó)非洲豬瘟的科學(xué)防控提內但供參考。Global Epidemic and Vaccine Rese關你arch Status of Afri道時can Swine Fever Viru影件s Genotype I StrainTh紅樂e African swine fever 城我virus has been introduced into China an船子d contaminated certain areas. Genotype線為 I strains have been found in domesti線議c pig samples，suggesting新嗎 that the virus populations act公低ually circulating in clinical sa低的mples are more complicated.雨們 Research on genotype I裡知 strains began after it was introduce都校d to Portugal in 1957. After years of r議亮esearch accumulation，a large number器分 of studies on the pr輛舞evalence and distributio少微n of genotype I strain，especial不輛ly the development of l哥化ive-attenuated vaccines based on ge樂術notype I strains，have been largely理都 documented and developed in for哥讀eign countries，but there is no de姐事scription in this regard in China. Ther子時efore，the author城雜 reviewed the current status 綠好of the African swine fever virus 什下genotype I strains and vaccine討男 research developement，hoping 動醫to provide a reference for the scientif暗工ic prevention and contr在為ol of African swine fever in China.全謝輛文下載鏈接：https://kns.cnki.net/kcms/用靜detail/detail.aspx?dbcode=CJFD&dbname=C林大JFDAUTO&filename=ZGDW202112015&unipla請師tform=NZKPT&v=2vpJqQNi66Hby1pq3vTe20K了子Oauu52mR6F-LqVPN6BLcUOMbZm關我Sw5vpffiQkl1Ono
2021-12-22
雲南省野豬源豬瘟病毒E2及5'NTR基因銀煙序列測定分析
豬瘟（CSF）是由豬瘟病毒（CSFV）感染豬引雨車起(qǐ)的一種(zhǒng)急性、熱性、接觸性傳染病，嚴重危害光匠我國(guó)養豬業健康穩定發(fā)展。本研究采用RT-就城PCR方法，對(duì)從雲南省甯相還洱縣2頭病死野豬體内分離到的2株CSFV毒株進(jìn)懂器行了E2及5'NTR基因擴關房增、克隆及序列測定；采用DNAMAN、MEG喝弟A6等分子生物學(xué)軟件，對(化玩duì)測得的序列與國(guó)内南西外參考毒株進(jìn)行了同源性分析及系統發(fā)育分身吧析。結果顯示：2株野豬源CSFV河資株E2、5'NTR基因同源性分别爲8事子7.2%、100%，與國(guó)内外參考毒株同源性分别爲81.為歌0%~97.6%、93.6%~98.5%，司高與我國(guó)強毒株Shimen株的同源性分别爲高少81.0%~81.2%、95.6%；2株野場錯豬源CSFV毒株E2基因屬于基因2.1亞型，5'NTR基因屬于動水基因2.3亞型。氨基酸序列分析顯示：其中一株分離費熱毒株的E2基因有6個氨基酸具有2.1d分子變異特征物少，另一株兼有2.1d和2.1b亞型分支特征，可能(néng)是老土2.1b和2.1d亞型的過(guò)渡毒株。結果表制文明，雲南省野豬源CSFV雖存在一定的遺傳衍化，但總體比較穩定。本研究爲雲南省這你CSF防控提供了分子流行病學(xué)依據，爲進(jìn)一步做好務拿(hǎo)分子變異等研究奠定了基礎。Sequenc人民ing and Analysi長錢s of E2 and 5會村9;NTR Genes of CSFV Derived from Wild動慢 Boars in Yunnan Provi紅妹nceClassical swine fever（CSF）is街窗 an acute，febrile and contact 得音infectious disease caused by c資件lassical swine fever virus（飛土CSFV），which has seriously en內和dangered the healthy and stable devel慢化opment of pig industry in山票 China. In this study，E2 and 5'事喝NTR genes of two CSFV strains isolated 水慢from two dead wild boars i個喝n Ning'er County of Yunnan Pr銀司ovince were amplified，cloned and 跳森sequenced by RT-PCR；the homo影視logy and phylogeny of th工少e obtained sequences and the referen門問ce strains were an慢習alyzed and compared北司 by DNAMAN，MEGA6.0 and other molecu到水lar biology softw讀討are. The results sh就輛owed that the homology of E2 行我and 5'NTR gene of the two strains 購會were 87.2% and 100%，respectively，which費新 were 81.0% to 97.6% an什熱d 93.6% to 98.5% respect紙科ively with the r北對eference strains，81.0% to 8房哥1.2% and 95.6% respect鐵費ively with Shimen virulen村習t strains in China；for the two strains，金拍E2 gene and 5'市南;NTR gene belonged to subtype 2.1 個空and subtype 2.3，re從海spectively. As indicated by 校湖amino acid seque對來nce analysis，6 amino acids with molec年動ular variation characteristics o子電f 2.1d subtype were observ機分ed in one strain，characteristics o通地f both 2.1d and 2.1b subt那動ypes were in another strai筆雪n，which might be a transitional s舞自train of the two subtypes. In conclusi理為on，the CSFV pre從著valent in wild bo吃劇ars in Yunnan Pr玩拍ovince was generally妹農 steady although a certa通劇in genetic evolution was dete就見cted. A molecular epidemiologic資木al basis was provided for prevent話請ion and control of CSF in Yunnan Prov月西ince，and a foundation was al算老so laid for further studies o筆道n molecular variation.全文下載鏈接：話雨https://kns.cnki.net/kcms/detail/de上海tail.aspx?dbcode=CJ火自FD&dbname=CJFDAUTO&filena下兵me=ZGDW202112007&uniplat雨機form=NZKPT&v=2vpJqQNi66ERRxm要空GNP7wH4P5HUgI2p6-yUNyfA我媽IzjEJR1xJdu5Cvr13hDIdzEpPA
2021-12-22
5株湖南豬圓環病毒1型全基因組測序與遺傳開新進(jìn)化分析
爲了解湖南豬群豬圓環病毒1型（PCV1）的遺傳變異秒廠情況，以PCV1陽性DNA爲模闆，使用PCR方法分兩(liǎng)段擴增劇輛PCV1全基因序列并進(jìn)行拼接。結果共獲得5個PC個離V1全基因組，其中3株全基因大小爲1759 bp，另外2關我株分别爲1758 bp和1760 bp。5株全基因組同源性爲98.4%~9木數9.7%，與國(guó)内外的PCV1全基因組同源性爲跳南98.1%~99.7%；ORF2序列同源性爲97.6明爸%~99.6%，與國(guó)内外的PCV1 ORF2同子訊源性爲96.1%~99.9%。系統發(fā)育樹顯示，5株PCV1均屬于同一分舞湖支，并顯示出一定的地理差異，但差異較小。結果表多區明，湖南省流行的PCV1毒株基因較爲穩定，分離株間差異較小。本研究爲湖南省些相豬圓環病毒病防控及相關研究奠定了基礎。Whole Genome Seque公新ncing of Five St離老rains of PCV1 in Hunan Pr兒司ovince and Analys坐少is on Their Genetic Evol錢好utionIn order to ide玩爸ntify the genetic variation of po雪間rcine circovirus type 1（PCV1）in pigs店男 in Hunan Province，taking PCV1 pos弟上itive DNA as a template，two section現廠s of PCV1 gene seq金離uence were ampli自很fied by PCR and th器花en spliced together to form the whole著技 genome. Five strains of whole 請子genomes were obtained，in which，thr看分ee were 1759 bp，and two were 1758 bp an也亮d 1760 bp，respectively. T要子he homology of the whole gen志答ome was 98.4% to 99.7%，which sha微話red the homology of 裡可98.1% to 99.7% with othe廠事r whole genomes of PCV1；the homolog睡熱y of ORF2 seque這對nce was 97.6% to 99.6短用%，which shared the homology of 96.1% to些頻 99.9% with other PCV1 ORF自問2. As indicated by the phylog店站enetic tree，all the five紅拿 strains belonged to the sam秒北e branch with slightly geographical年學 difference. In計呢 conclusion，the 算能strains were steadily prevalent北討 in Hunan Province with 廠月little difference. A foundation w訊算as thereby provided for future preven慢懂tion and control of PCV in現我 Hunan and for relevant st開畫udies.全文下載鏈接：https://kns.cnk筆物i.net/kcms/detail/detail.aspx?dbcode我費=CJFD&dbname=CJFDAUTO&file相老name=ZGDW202112006&uniplatform=為煙NZKPT&v=2vpJqQNi66HqJDejgO5KLs船低0EGxQ8L9WhpUhacoszGiKxaCrkOSuJB1p4Dd7-S費志Vjr
2021-12-22
2017—2020年廣西部分豬場送檢血清豬僞狂犬病抗體檢測
爲了解廣西豬場僞狂犬病毒（PRV）感染和免疫狀況，2017年1月—2020年1匠亮2月，對(duì)部分豬場随機采樣(y金報àng)送檢的血清樣(yàng)品書我，應用ELISA方法開(kāi)展PRV血清抗體檢測，并對(duì)檢測結果輛這進(jìn)行不同年份、不同季節、不同地區、不同生長(cháng)階妹術段豬群的統計分析。結果顯示：從時(shí分紅)間分布上看，2018年PRV gE抗體場陽性率（57場好.71%）和個體陽性率（24.75%）均最高，此後(h長刀òu)呈逐年下降趨勢，其中個體陽性率土快下降明顯（P＜0.05），2020年下降至6.14%；各年間的PRV gB抗體明日場合格率差異不顯著（P＞0.05），均在木票90%以上，而2019年的個體陽性率（88.71%）最低，與其他年份輛算差異顯著（P＜0.05）。冬季PRV gE和gB抗體個體陽性率件來最低，分别爲14.96%和89.35%，與其他季節差異明顯（P＜0.0睡店5）；冬季gE抗體場陽性率（42.97%）最低喝話，與春季、秋季差異不顯著（P＞0.店如05），但顯著低于夏季（P＜0.05），而gB抗體場合格黑做率一年四季差異不顯著（P＞0.05），均在9年務5%以上。從空間分布上看，廣西14個地男藍市豬場均存在不同程度的PRV野毒感染，其中玉林市最嚴重，場陽性中生率和個體陽性率分别爲69.51%和35.86%，業短而欽州市最輕，分别爲16.67%和4.07%；PRV gB抗體個體合格率化著普遍較高，均在84%以上。從不同生長(cháng)階段豬群上北兵看，後(hòu)備母豬、育肥豬麗業PRV gE和gB抗體個體陽性率均顯街劇著低于其他生長(cháng)階段豬群（P＜0.05），其中gE抗體個體陽性要睡率在13%以下，而gB抗體個體陽性率不這熱足80%。結果表明，近年廣西規模豬場PR朋購V野毒感染率較高，而疫苗免疫并不能(néng)完全道制阻止野毒株感染。建議通過(guò)PRV就章淨化和提高規模豬場生物安全水平等措施來控制其流行。本研究爲制定合坐劇理的豬僞狂犬病防控與淨化策略提供了依據。年新Detection of An木鐘tibodies against PRV in Serum S呢得amples Delivered by Some 木雪Swine Farms in Guangxi from 2017 to 些分2020 In order to investigate t土術he status of infectio能報n and immune of porcine pseudorabies v學會irus（PRV）in swin器房e farms in Guan人書gxi，serum samples randomly collected 司輛from some farms during Janu到校ary 2017 to December 2020 店近were detected for anti媽離bodies against PRV by ELISA，光車the results were summarized an綠話d analyzed according藍事 to different years，seasons，regio頻慢ns and pig populations at diffe外跳rent ages. The results show草章ed that the positive舊章 rates of antibodies against嗎些 PRV gE at farm level（57.71%）a是跳nd individual level（24.75%）wer訊熱e highest in 2018，then trended t白船o decrease year by year，錢日especially the individual one tha農道t even decreased 工費to 6.14% in 2020（P＜0.05）；the qualificat行低ion rates of an東開tibodies against PRV gB at farm level草但 were above 90% and the diffe月但rence was not significant i白西n all these years（P＞0.05），but the愛校 positive rate at the ind看頻ividual level was lowest in 2019（88.71中農%），and significantly different文短 from those in other years（P＜0.05）. T黑木he individual posit遠懂ive rates of antibodi亮師es against PRV gE and 了男gB were 14.96% and 89.35% respe畫相ctively，lowest in winter，an影刀d obviously dif得拍ferent from those i做農n other seasons（P＜0.05）；the posi畫訊tive rates of antibodies紙一 against gE at farm level去來 were lowest in winter（42.97%），and子西 not significantly differ如愛ent from those i問區n spring and fall（P＞0.05），but signif頻熱icantly lower than those in summ和錯er（P＜0.05），while tho請村se of antibodies against gB w離師ere above 95% and the di畫很fference through南身out the year was not significant地秒（P＞0.05）. For spatia到雪l distribution，PRV wild virus was pre的機sent in all the farms to different exte友花nt，most serious in Yulin City where t白就he positive rat司亮es at farm and i吧兵ndividual level were 業科69.51% and 35.86%，re懂讀spectively，and light森他est in Qinzhou City where w市村ere 16.67% and 4.07%，respectiv是生ely；The individual qualification rate就綠s of antibodies again放店st gB were all above為說 84% and generally h匠老igher. For pig populations at dif事山ferent ages，the ind暗拿ividual positive rates of拍和 antibodies against gE and gB in rese作制rved sows and finishing姐呢 pigs were significantly呢海 lower than those at other stages（聽舊P＜0.05），specifically，音計the individual 服雪positive rate of 子地antibodies against gE 自校was less than 13%，whi木紙le that of antibodies against gB was 數時less than 80%. In conclus鄉刀ion，the infection rate of PRV 坐看wild virus was relatively h從雪igh in large-scale farms都工 in Guangxi，which could not b日身e fully controlled by vaccination，so P能物RV purification and improvement 要校of bio-safety level in 她錢the farms were recommended. A basis 來愛was thereby provided for developing 站厭reasonable measures for P年也RV prevention，control an身站d purification.全文下載鏈接：https://kns就銀.cnki.net/kcms/d秒低etail/detail.aspx?dbcode=CJFD&dbname=CJ紅信FDAUTO&filename=Z黑快GDW202112002&uniplatform=NZKPT&v=2v體中pJqQNi66G6HrjqIrUp6YtHMbKZogQ0_UBQj相謝Yj2DaI3GF0Mz3ac3ErT月但gp8mhlAW
2021-12-22
固相萃取-超高效液相色譜法同時唱少(shí)測定豬肉中22種(zhǒng)磺胺類藥物殘留
爲探索一次性檢測豬肉中多種(zhǒng資她)磺胺類藥物殘留的檢測方法，建立了同時(短用shí)測定豬肉中22種(zhǒng)磺胺類藥物殘留的固相萃取就說-超高效液相色譜方法。樣(yàng)品用3%乙離文酸乙腈提取，MCX固相萃取柱淨化，水和甲醇淋洗，5%氨化甲醇洗脫，弟商氮氣吹至近幹，0.1%甲酸-乙公銀腈（9:1，V/V）溶液複溶，二極管陣列檢測器270 nm檢文不測。結果顯示：該方法對(duì)22種(體學zhǒng)磺胺類藥物檢測的線性關系良好(懂花hǎo)（R2＞0.999），定量限爲2.5~10.動資0 μg/kg，檢測限爲1.0~4.0 μg/kg；各藥物平均回收率爲83.2工高%~96.6%，批内和批間變異系數分别爲1.1%~3路離.5%和3.6%~13.4%。結果表明，該方法靈敏度高在笑、重複性好(hǎo)、成(chéng)本低廉，且操作簡單、快速，亮路可用于豬肉樣(yàng)品中多種(zhǒng)磺胺類藥物殘留的同時(sh好水í)檢測。Simultaneous De子慢termination of 22 Ki靜站nds of Sulfonamide Residues in Pork b睡音y SPE-UPLCIn order to develop a不黑 method for simult車白aneously detecting vario相個us sulfonamide residue視服s in pork，a solid-phase ex鐵說traction-ultra-high performance liq明黃uid chromatography（SPE-UPLC）method for從會 detecting 22 kinds of su生綠lfonamide residues in pork was estab照對lished. Samples wer線線e extracted by 3% acetic acid acetonit遠農rile，purified by MCX solid phas高樂e extraction column，rinsed by water 少醫and methanol，eluted 她事by 5% ammoniated methanol，dried by林森 blowing with nitrogen，and r海黑edissolved by 0.1% f熱醫ormic acid-acetonitrile（筆森9:1，V/V）solution，and then文樂 detected by a diod鐵地e array detector a現爸t 270 nm. The results showed 書微that the linear relatio哥男nship was good（R2>register.html
2021-07-26
不同非洲豬瘟病毒熒光PCR檢測試劑盒麗化在檢測豬肉及豬肉制品中的比較
爲了解不同非洲豬瘟病毒熒光PCR檢測試劑盒在檢姐放測豬肉及豬肉制品中的特征，采用國(g司算uó)内外6種(zhǒng)商品化兵高非洲豬瘟病毒實時(shí)熒光P離影CR檢測試劑盒，分别對(duì)我媽同一樣(yàng)本開(kāi)展穩定性試驗，對(duì)相同非洲豬瘟強陽性、匠員中陽性及弱陽性樣(yàng)本開(kāi)長遠展敏感性試驗。結果顯示：6種(zhǒng)試劑盒些鄉擴增穩定性良好(hǎo)；對(duì些不)強陽性樣(yàng)本，所有商品化試劑盒南朋均能(néng)檢出；對(du費文ì)中陽性及弱陽性樣(yàng)本，特别是弱陽性樣(yàng)本，不月快同試劑盒的檢出情況不盡相同。因此在實際應用中，應根據具體需求選擇合适制筆檢測試劑盒。本試驗爲一線工作者選擇使用不同商品化非洲豬瘟檢志我測試劑盒提供了一定參考。Compari拿光son of Different F通綠luorescent PCR Kits for Detection of A黑他SFV in Pork and算近 Its ProductsIn order to ide北年ntify the characteristics of d自議ifferent fluorescent PC東腦R kits for detection of章關 African swine fever virus（ASFV）in p通煙ork and its products，stability test wa西開s conducted for th家資e same samples by 6 domestic and for行火eign PCR kits and sensitiv爸大ity test for the samples 討還with strong，medium報拿 and weak positive ASFV. The r問用esults showed that雪子 all the 6 kits were with good stabi習謝lity；for sensitivity test，the stro讀請ng positive samples could be detected o聽愛ut by all 6 kits，but med自木ium positive and weak positive s大舊amples，especially the latter，t計風he results were quite different de雪資pending on different吃輛 kits. Therefore，an appropriate k鄉遠it should be selected我鐘 according to sp哥長ecific need in practice. Some 通拍reference was thereby provided for f在水ield workers to selec朋話t various kits.全文下載鏈接：https://kns.cnk資鐵i.net/kcms/detail/det農站ail.aspx?dbcode=CJFD&dbname=CJFDAUTO爸麗&filename=ZGDW20210702什愛1&v=2vpJqQNi66HdeB%25mmd2FairmIkTp%25mm看跳d2BpTLac8IKAFG3c7tBG4r%25mmd2B制就bxzu3K5KmKJj971CGEDq
2021-07-26
豬場紫外消毒技術研究進(jìn筆書)展
在養豬業發(fā)展進(jìn)程中，生物安全防控月開一直是人們關注的焦點。紫外消毒技術以消工空毒效率高、使用成(chéng)本低及安全性高等特點，木花在豬場生物安全領域開(kāi)始受到關注，并算大取得一些進(jìn)展。但是紫外消毒技術仍內海存在病原體滅活不完全等情況，其日為滅活效果是紫外消毒作業中最被(bèi)關心的問題。本河熱文就(jiù)影響紫外消毒效果的來街因素、滅活常見豬病原體所需紫外劑量以及現制紫外消毒在豬場的應用分别展開(kāi)論述。同時(shí)，針對船花(duì)研究發(fā)展中的不足之處進(jìn)行了總結和思城計考，以期爲提高豬場紫外消毒效果和飛生效率提供參考。Research Progress o家線n Ultraviolet Disinfection Technology 對低in Swine FarmsBiosafet技算y has always been a focu視麗s concerned during the development of說睡 swine industry，especially ultravi明快olet（UV）disinfection technology due生腦 to its high efficiency，low cost and hi子亮gh safety，and some results ha工裡ve been achieve金生d. However，the techn鄉匠ology could not fully in理很activate pathoge民刀ns sometimes，so its effect w個村as mostly concerned dur公電ing the work of UV disinfe飛可ction. In the paper，the 道器factors affectin為票g the effect of UV disinfection，its d腦姐ose required to inactivate comm少這on swine pathogens and聽鐘 its application in swine farms were d訊也iscussed. Meanwhile，the gaps availabl木好e in its development were sum黑愛marized and considered with a vi微樂ew to providing some referenc看我es to improve the effect and eff又兵iciency of UV disinfection in swine fa空還rms.全文下載鏈接：https://kns.cnki.net/kcms/d也唱etail/detail.aspx?db男遠code=CJFD&dbname商音=CJFDAUTO&filename=ZGDW202107019&v=2舞木vpJqQNi66Gnf%25mmd2F0V0z1tivdNkkdZxLg日科xO1XyItTazpPv29fsSFpDpJ0oD1Roayqf理算
2021-07-26
納米抗體篩選和表達技術研究進(jìn)展
納米抗體（nanobody，Nb）是在頻師駱駝血清中發(fā)現的一種(zhǒng)新型抗體，具有相對(duì)分子質量懂水小、穩定性高、親和力高、組織滲透性強以及可識别抗原縫隙表位、進(jìn)日草一步改造和表達等特點。Nb篩選技術分爲噬菌體員書展示、酵母雙雜交、mRNA展示以及高通量測序和質譜分析等4種(外坐zhǒng)，它們都(dōu)校計能(néng)夠快速篩選到特異街門性Nb。對(duì)具有高度親和力的Nb，多種弟電(zhǒng)表達系統均可以實遠間現高效表達。其中：原核表達最常用，其操作劇筆相對(duì)簡單、産量大、生産成(chéng)本低，但姐員要去除表達出的重組蛋白内毒素；酵母表達可以直接收集培養基上清純化，雜蛋藍拿白含量少，但可能(néng)存在過(guò)度糖基化問題；植物宿主可場時以大量表達Nb，但基因操作過(guò)西件程繁瑣，且分離純化Nb相對(duì)困難；哺乳動物讀好細胞生産抗體也是較常用的方式，但生産成(chéng)本較高。針對(duì)兒科每一種(zhǒng)篩選方法和表達系統目前沒(mé快妹i)有統一的評價标準，在選擇時(shí)，應以實驗室條件爲基礎，充分了解篩她鄉選對(duì)象的結構、功能(néng)和放麗用途，根據不同目的選用。本文對(duì)Nb結構特點、北光篩選及表達以及在動物疫病防治中的應用展開(kāi)論述，可爲相近工關領域研究提供技術支持。 Research Progress on the S還票creening and Expressi店家on of NanobodyNanobody（Nb），務我a new antibody found in都請 camel serum，is characterized by sma大鐘ll molecular weigh議微t，high stability飛資，high affinity，strong tiss近是ue permeability，and recognition of an慢公tigen gap epitopes，further modificatio通北n and expression. Nb screenin民冷g technology includes phage display，y朋遠east double hybridizat近不ion，mRNA display and high-throughput s到生equencing and mass sp亮農ectrometry analysis，all of which c區錯ould rapidly screen specific身讀 Nb. For the Nb with high a藍多ffinity，many expression systems c媽友ould be used to r師數ealize an effectively expression. Spe裡草cifically，prokaryotic紅樹 expression is mostly used due to it筆農s simple operation，mass production and飛南 low production cost，b影村ut the output of recombinant pr雪雜otein endotoxin should be removed；yea又用st expression could be used to direc山刀tly collect purifi雨答cation of medium supernatant，with議但 small content of miscellaneous protein線土，but excessive glycosylation m志睡ay be available；host plan坐短ts could express 城拍Nb in large quantity，but t藍麗he process of gene oper小腦ation is complicated and it is diffic又身ult to isolate 相玩and purify Nb；antibo志信dy produced by c業劇ells of mammals is also u員短sually used，but its producti草快on cost is high. No 見站uniform evaluation standard h我煙as been developed for購訊 all the above methods and expr如服ession systems till now購到. A decision should be ma短鐘de based on laboratory condi廠作tions and accor人做ding to correspond事電ing purpose provided會都 that the structure，function a但舊nd usage of the object to be screened 要慢should be well understood司懂. In the paper，the s個錢tructure，screenin輛舊g and expression of Nb a紅師s well as its application in the 暗問prevention and control of animal di鐘坐seases were discussed，which m小文ight technically拍得 support the study in 中在related fields.全文下載鏈接：知拿https://kns.cnki.n什開et/kcms/detail/detail.as子妹px?dbcode=CJFD&dbna他行me=CJFDAUTO&filename=ZGDW2021刀懂07018&v=2vpJqQNi66FLBlc7購就3hPRRzQ%25mmd2FUx6kmo9dZRrG呢黃7i2%25mmd2By6OlS1JcLyCcNp862n風低q3WlZI
2021-07-26
禽流感病毒實時(shí)熒光定量RT-PCR檢測方法的建立
牛結核病疫苗研究進(jìn)展
牛結節性皮膚病預警信息與風險管理
北京市延慶區農業局（北京市延慶區動物衛生監督如化管理局）關于以競争性方式選擇定期存款銀行的公告
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