不同接種(zhǒng)途徑對(duì)楓葉鴨病毒性肝大嗎炎抗體的影響
爲探讨不同接種(zhǒng)途徑對(duì)楓葉去光鴨病毒性肝炎（DVH）抗體的影響，選擇非免疫種(數東zhǒng)鴨所産的1日齡體質量均等的360隻健康楓葉雛鴨（A組），平均分上愛爲4組（飲水組、滴口組、肌注組、對(duì)照組）；選擇森志免疫種(zhǒng)鴨所産的360隻雛鴨（B組），也平均分爲4組（長資飲水組、滴口組、肌注組、母源抗體監測組）。在雛鴨5日齡時(shí)，除對(什站duì)照組和母源抗體監測組外，對(duì)其他組雛鴨進(jìn)行鐘音免疫接種(zhǒng)，分别于免疫後(hòu)5、10、15 d煙師，用ELISA方法檢測雛鴨抗體效價，并進(jìn)行攻毒保護員答試驗。結果顯示：A組的肌注組平均抗體效價（0.588±0.018都西）>飲水組（0.560±0.015）>滴口組（0.54是就1±0.009），雛鴨攻毒保護率依次爲9兵媽1.1%、85.6%、78.9%；聽南B組的肌注組平均抗體效價（0.513±0.021）>飲錢紙水組（0.484±0.051）>滴口組（0.466±0.生城014），攻毒保護率依次爲72.2%、知木66.7%、60.0%，均低于A組。結果表明：無論有無母源抗體雨舞幹擾，肌内注射的免疫效果最好(hǎo)，其次是滴友明口，飲水的免疫效果較差；母源抗體化為可幹擾疫苗的免疫效果。因此，需根據使用疫苗種(zh關舞ǒng)類和實際情況，選擇最合适、最有效的免疫途徑，同時(shí)做好(hǎ現嗎o)母源抗體監測，選擇最佳免疫時(shí)間。本研究爲鴨場免疫途徑的中知選擇提供了參考。Effect of Differe嗎又nt Vaccination Methods on t哥遠he Antibodies against Viral H算來epatitis in Maple一個 Duck In order to study t錢紙he effect of different va坐海ccination methods on the antibodies aga雨舞inst viral hepatit一問is（DVH）in maple ducks，話們360 one-day-old healt嗎線hy maple ducklings with equ公術al weight（group A）born by unvaccinate購民d ducks were divided into校她 four groups on av服高erage：drinking water，oral drop，intram暗離uscular injection and cont北術rol group；360 duckl媽線ings born by vaccinated ducks（grou公吃p B）were also equally divided into 聽森four groups on average：drinking wate小月r，oral drop，intramuscular inj書購ection and maternal anti裡市body monitoring. Except th嗎街e control group and maternal做分 antibody monitoring group短體，all the ducklings were va現也ccinated when they were five da風黑ys old，their antibo著愛dy titers were 湖做detected by ELISA method 雜南at 5，10 and 15 da林暗ys after vaccination離農，and the virus 們去challenge test was c中器arried out. The results showed t街嗎hat，for group A，the avera樹吃ge antibody titers were as foll時長ows：the intramuscular信森 injection group（0.588±山鐵0.018）>drinking water group（0.560±上動0.015）>oral drop group（志農0.541±0.009），and th請拍e protection rates were 91.1%，85.6%物微 and 78.9% respectively；for group B請黃，the average antibody titers were農關 as follows：the intram那山uscular injection group（0.來少513±0.021）>drink國湖ing water group（0.484±0.0懂身51）>register.html
2020-07-17
雲南省香格裡(lǐ)拉市豬腸氣泡病病例學我的組織病理學(xué)觀察與病原檢測麗東
雲南省香格裡(lǐ)拉市某藏豬場育肥豬出欄屠宰時(shí)，陸續發(fā)現部北民分個體小腸腸系膜漿膜下有大量葡萄串狀透明月樂無味氣泡，病因不明，但病豬在仔豬階間風段有腹瀉病史。爲調查發(fā)病原因和緻病機理，以便提出防控措施，采集該養豬師妹場4頭患病豬和1頭正常豬的抗凝全血、小腸組織和腸系膜淋巴結，進(劇這jìn)行病毒核酸PCR擴增、細菌分離培養和組織病理顯微觀察。在患病豬中，道算未檢出與腹瀉有關的豬瘟病毒、豬流行性腹瀉病毒、傳染性胃腸炎病毒和輪狀你跳病毒核酸；血瓊脂有氧和厭氧培養均隻分離到大和海腸杆菌，并排除常見緻病血清型及常見毒力基因；組織病理檢查發站現(fā)現，腸黏膜下層有大量空泡，腸腺及其杯狀細雜件胞體積明顯增大，組織間隙擴大，腸系膜及麗訊其淋巴結分别見嗜酸性粒細胞和巨噬細胞增多，病變腸系膜淤血。購睡分析認爲，該病病因應考慮養殖區域海拔較高，豬腸内大腸杆菌異常發(fā)酵産氣的學什可能(néng)，但如何防治需通商進(jìn)一步研究。 Histopathological Observat個著ion and Pathogen Detect匠兵ion of Porcine Intestine Pneumatosi通土s Cystoides in Shangri-La City of Yunn是雨an ProvinceA large number of 花務grape-shaped transparent odorless街自 bubbles were successive河老ly found under ser還服osa of small intestine m影東esenterium in some fattening pigs錯紅 in a Tibetan pig farm in Shangri-La C公些ity of Yunnan Province when the pigs 睡草were sold for slaughtering，with 舞人unknown causes，while 亮服the sick pigs had suffer我下ed from diarrhea中這 at the piglet st西民age. In order to investi舊媽gate the cause and pathogenesis so 飛資as to put forward pre兵媽vention and contr站時ol measures，the anticoagulan愛理t whole blood，small intestine tissues件山 and mesenteric lymph著熱 nodes were collected from fou音行r sick pigs and one normal pig for海秒 PCR amplification of viral n她月ucleic acids，bacterial isolation an金計d culture and micros裡業copic observation of histopatho又明logy. No any classical swine fever vir朋土us，porcine epidemic diarrhea virus，t一男ransmissible gastroenteri對我tis virus and rotavirus nucleic厭師 acid related to diarrhea were 分信detected；only Esc國民herichia coli was isolat坐長ed by blood agar aerobic an家件d anaerobic culture，and th算請e common pathogenic serotypes a術請nd virulence genes were e為麗xcluded；the following symptoms were f是快ound through patho河爸logical examination such as a la事錯rge number of vacuole窗要s in the intestinal submucosa，th一短e increased volume of intes銀費tinal gland and its goblet cel通員ls，the widened space among去購 various tissues，the increased日兒 eosinophils and還拿 macrophages in the mesentery and i下道ts lymph nodes，and congestion in th公農e diseased mesentery. It was 廠子concluded that the disease 技金was likely to be c我好aused by higher altitude，ab和行normal fermentation and air p學下roduced by E. coli，and it was 高報necessary to continue to s朋窗tudy how to prevent and control it.美水 全文下載鏈接：https://kns看來.cnki.net/KCMS/detail/det朋話ail.aspx?dbcode=CJFQ&錯時dbname=CJFDAUTO&filename車雪=ZGDW202007018&v=MjQ2OTMzcVRyV00xR用藍nJDVVI3cWZZT2RvRnl6a1Zydk1跳行QeXJQZWJHNEhOSE雨見1xSTlFYklSOGVYMUx1eFlTN0RoMVQ=
2020-07-17
牛白血病的危害及防控策略
牛白血病是牛白血病病毒（bovine le相請ukemia virus，BLV）感染引起(qǐ)的牛最常見的腫瘤性疾病之水水一，對(duì)養牛業和人類健康的潛在威脅較大。本文分别河請從病原學(xué)、流行現狀、危害及潛在風險、診斷和防控策略等方事這面(miàn)概述了BLV及其引黑高發(fā)疾病的研究進(jìn)文房展。BLV自首次發(fā)現以來，已廣泛流行于世界各國(guó)，并很小西容易跨物種(zhǒng)傳播，且兒計所有感染動物都(dōu)會(huì)終身帶毒，引中煙起(qǐ)廣泛的免疫功能(néng)異常，從而導緻産奶量降低、産肉分錢量下降和動物流産。此外，有實驗已證實了在人類乳腺癌、肺癌組織和民也部分人類血液中有BLV的存在。業我目前尚無有效治療和預防BLV感染的藥物和疫書樂苗，因此準确診斷和清除BLV感動鄉染牛是控制牛白血病最有效的措施之一。目前常用的診斷方法包括血清學(員還xué)抗體檢測和前病毒DNA檢測，主要的防控策略包括淨商輛化、通用的牧場管理與生物安全防護以及阻斷向(xi白短àng)人類傳播的途徑。綜上所述，應嚴格落實上述防控策略，加強診斷方法研讀土究和監測，做到早發(fā)現早處理。 Study西身 on the Hazards of Bovine Leukemi美體a Virus and Relevan公窗t Control MeasuresAs one of the common 制都neoplastic diseases in cattle caused 志老by bovine leukemia virus（BLV光錯），bovine leukemia ha公視s posed a great算河 threat to cattle industry and human h讀靜ealth. In this paper，the rese身兒arch progress on BLV and corr飛件esponding disease caused therefrom w金錯as summarized in terms of etiology，p嗎費revalence status，hazar店站ds and potential risks喝著，diagnosis，prevention and control mea開視sures，etc. BLV has been widely spread a房下ll over the world since it was first f空長ound，which is prone to cross-species t書錯ransmission，and is carried by a飛機ll infected animals all life，re理森sulting in abnorm日習al immunity and cau紙商sing the reduction of milk and 機習meat production a車錯s well as abortion. I和理n addition，it was confirmed by 自現some experiments that 就林BLV appeared in human breast c匠相ancer tissue，lung cancer tissu唱她e and some of human blood. However，no e習湖ffective drugs or 舊體vaccines were available to prev新樹ent and control BLV infection，so th黑離e most effective measure was to accura鐵一tely diagnosis and eliminate all infect兒見ed cattle. Serolog金來ical antibody detection不風 and proviral DNA detectio時吃n were commonly used at present，and 那好the prevention 離又and control measu技年res mainly included disease北她 purification，general manageme來輛nt and bio-safety pr都聽otection of pastures，and block數是ing all pathways of human可多 infection. In short，it was 朋體necessary to implement the above姐為 mentioned measures，strengthen the mo姐土nitoring of BLV，so as t兒機o guarantee“early detection a飛厭nd early disposal”.全文下載鏈接：弟空https://kns.cnki.net/KCMS/detai男動l/detail.aspx?dbcode=CJF慢紙Q&dbname=CJFDAUT用影O&filename=ZGDW202007017&v=多鐘MDMwMDNQZWJHNEhOSE1xSTlFWTRSO錢地GVYMUx1eFlTN0RoMVQzcVRyV00xRnJDVVI3cW腦醫ZZT2RvRnl6a1VMN01QeXI=
2020-07-17
布魯氏菌病研究進(jìn)展
布魯氏菌病的早期診斷及鑒定是該病防控的重了睡要環節，其診斷技術主要包括細菌微公學(xué)診斷技術、血清學(xué)診斷技術以及分子生物學(xué)診近鐘斷技術。布魯氏菌病防控應堅持預防爲主訊農的策略，其免疫主要以弱毒活疫苗爲主，随著(zhe)分子生物學(xu喝銀é)及重組DNA技術的發(fā)展，基因工程疫苗成(chéng)爲近年研究熱點行錯，但目前尚未有鑒别疫苗免疫和自然感染的鑒别街媽診斷技術及相應的疫苗制品。本文對(d森放uì)布魯氏菌病的病原學(xué)、流行病關森學(xué)、診斷技術和疫苗研究進(jìn)展等方面(miàn)進(j家樹ìn)行概述，以期爲建立靈敏性間南高、特異性強、高效便捷且易推廣的布魯氏菌病診斷技術和研發(內大fā)更加安全有效的布魯氏菌病疫苗提供基礎。Resear樹外ch Progress on Brucel行能losisIt is the ke商間y to prevent and control bruc動計ellosis by early diagnosis and ide吃他ntification that mai輛很nly include the technologie這謝s of bacteriology，serology 歌那and molecular biology. T長兵he brucellosis vaccines have bee友南n playing an important role due to the 煙玩strategy of“Preventi拍短on First”，and the live attenuat北南ed vaccines are 照作commonly used at p間錯resent. With the development of 紙和molecular biolo風影gy and recombinant DNA technology，雨去genetic engineering vaccine has d鐘路eveloped rapidly and become a hots刀有pot in recent years. Howeve上聽r，no any differenti藍火al diagnosis technologies綠南 and corresponding vaccine離冷 products for distin人暗guishing vaccine im事用munity from natural infection匠子 have been available間她. In this paper，the etio線這logy，epidemiology，diagnosis technolo男去gy and brucellosis vac電在cine development status 理間were summarized，which laid a foun不站dation for the estab體拿lishment of a popular輛西ized diagnosis technology wi門計th high sensitivity，speci綠家ficity and efficiency，as 校務well as for the d土花evelopment of the more effective and sa放上fer vaccines.全文下載鏈接：https://kn麗這s.cnki.net/KCMS/信畫detail/detail.aspx?dbcode=C東讀JFQ&dbname=CJFDAUTO&fil拍商ename=ZGDW202007事時016&v=MDA2MzExVDNxVHJXTTFGck筆用NVUjdxZllPZG9GeT多機NoVzczQVB5clBlYkc0SE5ITX錢車FJOUVZb1I4ZVgxTHV4WVM3RGg=
2020-07-17
新型冠狀病毒研究進(jìn)展
自2019年12月新型冠狀病毒肺炎（coronavirus diseas討跳e 2019，COVID-19）暴發(fā)以來，世界各國(guó)相繼出現好也了大量新型冠狀病毒（severe acute respirato了黑ry syndrome coronavirus 2，笑身SARS-CoV-2）感染病例。SARS-CoV-2對制件(duì)全球公共衛生安全造成(chéng)了巨大威脅。疫情發(fā)生又雨以來，相關科研人員從SARS-CoV-2的病商讀原學(xué)特點、流行病學(xué)特征、病原學(xué)檢測、治療與輛他愈後(hòu)症狀等方面(miàn)進(jìn)行了研究。本但吧文對(duì)上述幾個方面(miàn)的研究成(chéng)果進為醫(jìn)行了彙總。研究發(fā)睡說現：SARS-CoV-2以其遺傳匠輛多樣(yàng)性和重組頻繁的特點不斷産生變異體，以更大時黃的傳播力感染人群；目前已知的2種(zhǒn事關g)傳染途徑爲跨物種(zhǒng)傳播和人際傳播，其中飛沫傳雜工播和接觸傳播是人際傳播的主要傳播途徑；感染人群遍布各個年齡段，其中輛哥患有慢性疾病的老年人更容易發(美水fā)展成(chéng)危重病人；臨床檢測時(shí)聯合核酸和抗原抗體農你檢測，將(jiāng)有助于提高SARS-CoV-2感染的檢出率并排除兵和其他冠狀病毒感染的可能(néng)性；目前尚未有治療COVID-19的特效藥物事讀，臨床上廣泛使用廣譜抗病毒藥物或中藥爸水來治療各種(zhǒng)症狀；全球各國(guó)正在加緊研數電發(fā)SARS-CoV-2疫內什苗，部分疫苗已進(jìn)入臨床試跳河驗階段。本文有助于全面(miàn)了解SARS-制中CoV-2的研究狀況，也爲其後(我內hòu)續研究及防治提供了參考。Research Pro電書gress on SARS-CoV-2見很A large number of cases infected wi微店th severe acute respiratory syndrome現會 coronavirus 2（SARS-CoV-2）have appe麗坐ared in many countries since Decembe員師r 2019 when the outbreak o鐘海f coronavirus disease 2019（COVID-19）o家雜ccurred，which has posed h村舞uge threat to global public h拍區ealth. SARS-CoV-2 has bee還紅n studied by relevant女男 researchers based on its e不說tiology，epidemiological chara哥長cteristics，etio相為logy detection，therapy and prog跳很nosis since the outbreak，and the 媽唱research results were summarize劇線d in this paper as follows：varian看些ts had been produced 公好by SARS-CoV-2 due to its ge現飛netic diversity and freque區年nt recombination，which had infected an 路友increasing number of people事議 with stronger t都舞ransmission；two pathways includi大內ng cross-species and interpersonal 和劇transmission had been known店答 at present，in which，the m關音ain pathways of interperson媽對al transmission includ務謝ed droplet and contact transm有離ission；people at all 亮個ages might be infected，especially 畫和the elderly with chronic disea線謝ses were more likely to become crit科工ical patients；the detection rate might 制化be improved by the combination o票制f detection of nu報視cleic acid and antigen antibody that 兵姐could eliminate the pos年作sibility of other cor現銀onavirus infection；no effective drugs 腦和for COVID-19 had been available器是 at present，instead，broad-spectr資鐘um antiviral drugs or traditional Chi道姐nese medicine were widely used in匠要 clinical practice；the 慢木development of vac冷高cines against SARS拍音-CoV-2 had been sped書老 up by all countri年我es，and some vaccines had been at 書暗clinical trial s弟一tage. The statu司來s of development of SARS鐘熱-CoV-2 were sum西遠marized in this paper，which also新她 provided some references f爸窗or future study and control厭就 of it.全文下載鏈接：https://k高中ns.cnki.net/KCMS/detail/detail器員.aspx?dbcode=CJFQ&dbname=CJFDAUTO輛麗&filename=ZGDW202007015&v=MjY0MzRx訊綠VHJXTTFGckNVUjdxZllPZG9GeTNoVkw匠哥zUFB5clBlYkc0SE他下5ITXFJOUVZWVI4ZVgxTHV4WVM3RGgxVDM=
2020-07-17
上海市某奶牛場牛型結核菌素皮内變态反應疑似結果影響因素分少也析
爲評估奶牛年齡分布、棚舍位置、妹訊胎次、泌乳等因素，對(duì)牛型結核菌素（開能PPD）皮内變态反應試驗特異性的影響，對(d習笑uì)上海市某奶牛場2031頭奶牛的PPD皮内喝站變态反應試驗初篩結果進(jìn)行區地分析和回顧性問卷調查，并利用Epi infoTM 7軟可能件，對(duì)初篩結果和問卷調查結果進(jìn)行單因素分析。初篩和問下章卷調查結果顯示：該奶牛場PPD皮内變态反應試驗初篩疑似反志門應率爲15.75%，疑似反應主要集中在2歲以下、0~2男計胎的青年奶牛，其中1.5~2歲青牛奶牛疑似反應率最高；随著(zh快山e)年齡、泌乳量和胎次的增加，疑車金似反應率呈下降趨勢。單因素分析結果顯示：未配種(zhǒng)奶牛（OR離大=2.02，P＜0.01），未懷孕奶牛（OR=1.37，P＜0.01），未泌年吃乳奶牛或幹奶牛（OR=3.72，P你吃＜0.01）是導緻奶牛場出現PPD皮内變态反應討村試驗疑似反應的危害性風險因素。結果表明：2歲以下青年奶牛易出現P答地PD皮内變态反應試驗疑似反應，呈現出顯著的年齡商業相關性；是否配種(zhǒng)、懷美從孕以及泌乳量多少均能(néng)夠影拿南響PPD皮内變态反應試驗的特異性。建議奶牛場將(jiāng)工一犢牛結核病首次檢測日齡提前至90日齡以内，并對(duì)初篩出現疑似可看反應的奶牛立即隔離，采用γ-幹擾素試驗和PPD比較皮内變态反應試驗的通服平行檢測策略進(jìn)行确診。本研究全面(mià跳都n)了解了奶牛場初篩疑似反應奶牛的個體特征和風險因素，有助于提高檢測方法的敏感離厭性，減少假陰性。 Analysis on the Inf國還luence Factors for些和 the Suspected Reaction to Int哥離radermal Allergy Test of Purified P師線rotein Derivative in 暗人a Dairy Farm in Shanghai CityIn o匠雜rder to evaluate the age，跳技shed location，parity and milk prod她動uction of cows and oth輛子er factors influe體科ncing on the sp市飛ecificity of intradermal allergy test o路購f purified protein 子算derivative（PPD），the preliminary t制爸est results for 2 031 cows in爸我 a diary farm in Shanghai綠鐵 City were analyzed，and 會就a follow-up questionnaire was carr輛聽ied out，single-factor anal和女ysis on the results was carried out b放劇y use of the software of Epi inf南拿oTM 7. It was found that the suspected光坐 reaction rate of PPD wa為票s 15.75%，mainly in the young c輛靜ows less than 2 years old and with家文 0 to 2 parities，especially the 物司young cows at 1.5 to 2 years o大自ld；the rate trended to decrease w很藍ith the increasing age，milk production 玩風and parity. It was found tha門分t，by the single-factor玩校 analysis，the suspected reaction資路 was caused by the hazardou快習s risk factors inclu坐聽ding unmated cows（OR=2.02，P＜0.01），no海業n-pregnant cows（OR=1.37，P＜0.01），unlacta愛地ting or dry cow公民s（OR=3.72，P＜0.01）.討答 The results showed that the s白國uspected reaction was likely produced北拿 in young cows less 弟和than 2 years old，which was obv說跳iously related to age；the 遠金specificity of the reaction coul小劇d be influenced b師但y whether the cows had been mated道北 or pregnant and the amoun暗玩t of milk production. It was suggested拿喝 that the first time o信筆f tuberculosis detecti長志on should be brought forward withi老劇n 90 days old，the cows with suspe制一cted reaction should be quar線錢antined immediately for confirmation路答 by the parallel det睡離ection strategy of γ-interferon test 知吃and intradermal得遠 allergy test of PPD. 全文下載鏈接：https票報://kns.cnki.net/KCMS通生/detail/detail.aspx?dbcode=CJFQ&dbname=數業CJFDAUTO&filename=ZGDW2煙資02007007&v=MDA0NzN5M2hVTHZCUHl錯空yUGViRzRITkhNcUk5Rlk0UjhlWDFMdXhZUzdEaD師水FUM3FUcldNMUZyQ1VSN3FmWU9kb0Y=
2020-07-17
上海市僞狂犬病病毒相關毒力基因分子變異分析
爲了解僞狂犬病病毒（pseudorabies virus快朋，PRV）4種(zhǒng)主要毒力基因（gB機信、gC、gD和gE）的分子特征和變異情去議況，采用PCR技術，對(duì土新)2010—2015年分離到的28株PRV進(jìn)行基因擴增和測序。結果作要顯示：2010—2015年分離毒株的gB、gC、gD和gE基因那還均存在多位點突變，且位于抗原表位區；與2010年分離毒株的gD基因相綠的比，2012年後(hòu)分離毒株存在6個連美商續氨基酸插入。基于gB、gC、gD和gE基因的進(jìn)化樹顯示：上海市劇人2010年分離的4個毒株與2012年以後(hòu)分離的毒株雖屬于同一大的分支地計，但與經(jīng)典毒株親緣關系近，處于同一小的分支中；2我會012年分離毒株與2011年後(hòu)國(guó舞對)内變異株親緣關系近，處于另一分支中。這(zhè)些毒力低樂基因抗原表位區域氨基酸的突變可能(né兒錯ng)導緻其毒力及抗原性發(f聽地ā)生改變；2012年以後(hòu)分離毒株均爲變異株，并已成(chén姐大g)爲上海市的主要流行毒株。本研究爲PRV分子緻病機是低制及分子流行病學(xué)研究提供了依據。 Molecul愛近ar Variation Analysis on Virulence 文在Genes Related to能照Pseudorabies Virus in Shanghai Cit小嗎yIn order to identify the 錢中molecular characteristics a媽關nd variation of major 影科virulence genes（gB，理花gC，gD and gE）of pseudorabies virus（PRV）土我，28 strains isolated from 2志北010 to 2015 were amplified 民慢and sequenced by PCR assay. The res中我ults showed that空吧 multi-site mutations appeared in呢如 the epitope zones of all th錢劇e genes；compared to the 理懂gD gene isolated in 2010，six consecu時用tive amino acid in業物sertions appeared in 日錢the strains isolated since 2012. Based短報 on the genetic evolution trees of 路錢gB，gC，gD and gE g為機ene，the four st舊森rains isolated in 2010 belonged to錢船 the same big branch as the strain林舊s isolated since 201舊業2，but they were c了畫losely related to the cl匠家assical strains，which were in the sa師師me small branch；the st路開rains isolated in 2012 were closely音購 related to the窗船 domestic variant strains isolated sinc票學e 2011，which belonged to another branc什船h. The virulence and antig照吃enicity of the gene視樂s might be changed by the mut紙購ations of amino acids 讀問in the epitope zones.票得 All the strains isolated since 2習到012 were variant，and h黃月ad become prevalent in Shanghai Ci北他ty. Therefore，some 工劇references were provided for the務笑 study of PRV molecular pathogenesis an業鄉d molecular epidemiology.全文下載鏈接：ht吃藍tps://kns.cnki.net/KCMS/detail/de計自tail.aspx?dbcode=CJFQ&dbname=CJFDA畫明UTO&filename=ZGDW202007005&v=MTA5NzN門通5M2hVci9CUHlyUGViRzRITkhNcU黑腦k5RllZUjhlWDFMdX習下hZUzdEaDFUM3FUcldNMUZ開家yQ1VSN3FmWU9kb0Y=
2020-07-17
2018—2019年我國(guó)開子部分地區豬肺炎支原體多位點序列分型
爲了解我國(guó)豬肺炎支原體種(zhǒng)群結構和進(jìn)化關系，得分2018—2019年采集山東、廣西、河南、江西、雲南、天津睡黃、湖南等地豬組織樣(yàng)品拿民1 242份，進(jìn)行豬肺炎支原體熒光坐嗎定量PCR和巢式PCR檢測，并對(duì)測序确認的87份陽性樣(yàn嗎暗g)品，采用adk、rpoB、tpiA 3個管家基因作爲分錯拍型參考進(jìn)行多位點序列靜紙分型（MLST），并對(duì)3個件自管家基因均擴增成(chéng)功的陽性樣(yàng)品進(jìn)行p146基火們因分型，分析我國(guó)豬肺炎支原體種(zhǒng)群船問結構，并結合pubmlst數據庫公布的151個序列類型影件（ST）進(jìn)行eBURST分析。結果顯示：有放放24份陽性樣(yàng)品的3個管家基因全部擴增且測序成(ché通小ng)功，共分爲10個ST，均爲國(guó)内外首次報道(呢務dào)；10個ST分爲3個克隆複合體（CC）和4個獨特型，其中ST128占4海現1.7%（10/24），樣(yàng)品來自江蘇、河南和廣西，爲森月本次檢測的主要流行型。結果表明，我國(guó)豬肺炎支原體分布較微光廣，具有一定的基因型獨特性。本研究爲我國(guó)豬肺炎支原來身體的流行病學(xué)數據庫研究提供了新資料，有助于掌握飛習國(guó)内豬肺炎支原體基因型分布情況，進(jìn)一步完善了豬肺來劇炎支原體流行病學(xué)數據庫。 M民麗ulti-locus Sequence T個得yping of Mycoplasma hyopneumoniae in頻花 Some Regions of Ch地舊ina during 2018 to 20拍雪19 In order to investigate the gene學錯tic characteristics and evolutiona跳體ry relationship of Mycopl動見asma hyopnumoniae（M. hyopnu弟們moniae）in China，1 242 答坐swine tissues were collected 但家during 2018 to 2019 from Shan商筆dong，Guangxi，Henan，Jiangxi，Yunn花筆an，Tianjin and Hunan fo分草r detection of M. hyopnu民身moniae by real-tim商要e PCR and nested PCR花近，and 87 positive sampl愛算es were confirmed. DNA 還街was extracted from positive samples a關答nd classified according 了妹to p146 gene and multi-locus seq離白uence typing（MLST）with adoption of 3 h家這ousekeeping genes i業拍ncluding adk，rpoB and tpiA，then the po你南pulation structure of M照術. hyopnumoniae in Chin市腦a was analyzed，and eBURS站校T analysis was carried o鐵但ut in combination of 151舞舊 STs published in pubm公場lst database. Th草習e results showed that all街吃 the three housekeeping 學花genes of 24 posit子離ive samples were su時慢ccessfully amplified and sequenced，愛會which were divided int快人o 10 STs and firstly repo做民rted in the world，invol視鐵ving three clone老器 complexes（CCs）and four s得時ingletons，in which，ST128 wa得民s prevalent and accou大如nted for 41.7%（10/24）and 道些the samples were collected from Ji通著angsu，Henan and Guangxi. It wa學窗s concluded that M. hyop媽舞numoniae spread widely with unique gen爸行otypes to some extent. There劇草fore，some new data was provided 又他for the study on epidemiologica說離l database of M. hyop見醫numoniae，which contr機內ibuted to the i亮員dentification of its d錯化istribution and furt件湖her enriched relevant data花謝base.全文下載鏈接：https://kns.畫著cnki.net/KCMS/detai腦到l/detail.aspx?dbcod兵為e=CJFQ&dbname=CJFDAUTO&filen路風ame=ZGDW202007004國裡&v=MTQ2NDlOSE1xSTlGWUlSOGVYMUx南可1eFlTN0RoMVQzcVRyV00xRnJDVVI3cWZZT2RvR如呢nkzZ1ZMektQeXJQZWJHNEg=
2020-07-17
A型流感病毒分型基因芯片在流感病毒各亞型監測中的應用
爲驗證自行研制的A型流感病毒分型基因金在芯片方法在實際中的應用效果，了解A型流感病毒各亞型在說不我國(guó)不同地區和不同宿主中的分布文上情況，對(duì)我國(guó)20聽可個地區、不同宿主源（雞、鴨、鹌鹑、鴿子、豬、人等）拭子和月些組織樣(yàng)品共16 852份進(資紅jìn)行了檢測。利用自行研制的A型流感內自病毒分型基因芯片方法，對(duì)樣(yàn校厭g)品進(jìn)行RNA提取、多重快新不對(duì)稱RT-PCR擴增、慢司芯片雜交、洗滌和掃描分析；同時(shí)將(jiāng)基因芯片答市檢測陽性的樣(yàng)品，用普紙多通RT-PCR擴增後(hòu)進(jìn)行序列測定。結果顯示：利用該黑金方法檢出陽性樣(yàng)品2 582份，總算體檢出率爲15.32%（2 582/16 852），筆內共檢測到H1N1、H2N9、H3N2、H4N6、H5N1、H7N1、H7N9遠森、H9N2、H10N5、H16N3等10個亞型，且技身均與陽性樣(yàng)品測序結果一緻。結果表明，該方法可準确檢測不務弟同地區、不同宿主中的A型流感病毒不同亞型。同時(shí)，該方法能(néng)店拍在同一張芯片上準确鑒定A型流感病毒的多種(zhǒng)亞型，解決了其他常規方說化法無法檢測已發(fā)現和可能(néng)發(fā)生變異的所有亞型議湖的棘手問題，從而爲A型流感診斷和去生分子流行病學(xué)監測提供了一種(zh拍志ǒng)新技術。Application of 區跳Influenza A Viru村雪s Genotyping Microarray in Surve弟西illance of Influenza Virus Subt新們ypesIn order to verify the effect 計懂of influenza A virus genotyping microa場家rray in practice，and to mak友謝e clear the distribution道人 of all subtypes of influenza A virus 民新in various hosts in different是路 regions in China話靜，16 852 swabs and tissu長為e samples collect家說ed from different hosts（chickens我爸，ducks，quails，dove，p我業igs and human）in 20坐票 regions were tested，and then RNA ex醫能traction，multiple asymmetric RT-光書PCR amplification，chip hybridization報慢，cleaning and scanning analysis were 業日carried out by the self-developed genot業門yping microarray. Meanwhile，the裡內 positive samples were amplified by 去科conventional RT-PCR followed 動土by sequencing. The resu工東lts showed that 2 5厭近82 positive samples w和明ere detected out，with a total det化線ection rate of 1日城5.23%（2 582/16 852），in木遠volving 10 subtypes including H1N相慢1，H2N9，H3N2，H4N6，H5N1，H7N1，H7N9，H9N2，很友H10N5 and H16N3，which was 綠間consistent with the result of c說樂onventional RT-PCR. It was conclude外聽d that different su術吧btypes of influenza A virus件房 could be detected in various hosts i空如n different regions in China by the g我錢enotyping microarray t藍學hat could also accurately id離錯entify various subtypes in t弟金he same microarray and solve the 朋知difficulty in detecting all ident紅空ified or variant subtypes compared to t空區he conventional methods，so a new tech錯票nology was developed for confirmation 得長of influenza A virus and molecular epi飛短demiological surveill短藍ance.全文下載鏈接：https:坐南//kns.cnki.net/KCMS/detail/detai煙唱l.aspx?dbcode=CJFQ&dbname=C議和JFDAUTO&filename=ZGDW202005021&體森v=MDQ3NDZHNEhOSE1xbzlIWllSOGVY拍到MUx1eFlTN0RoMVQzcVRyV00xRnJDVVI3cWZZZV黑車pzRnl6aFViM0xQeXJQZW錯做I=
2020-06-04
豬弓形蟲實時(shí)熒光PCR檢測方法的建立爸身與應用
爲建立一種(zhǒng)快速、敏感了光、特異的豬弓形蟲檢測方法，根據弓玩鐘形蟲保守基因序列，設計一套特異性空票引物和FAM熒光素标記的MGB探針；通過(guò)對(du章麗ì) PCR反應體系和反應條件進(jìn)行優化篩選，建立了豬能呢弓形蟲實時(shí)熒光定量PCR檢測方法，并對(duì)此PC我看R檢測方法進(jìn)行了特異性體器、敏感性、重複性試驗；利用所建立的方法子雜對(duì)60份疑似弓形蟲感染的臨床樣(yàng)品進(jìn)行了檢測紙窗。結果顯示：建立的豬弓形蟲實時(shí)熒光定量PCR檢測厭子方法在101~107拷貝/μL模闆範火工圍内有很好(hǎo)的線性關系；對(du森綠ì)弓形蟲重組陽性質粒出現陽性擴增信号，但對(duì)陰性對(duì)照的水和藍資其他7種(zhǒng)病原對(duì)照未擴增出了讀特異性曲線；最低檢測模闆濃度爲10拷貝/μL；自60師件份疑似豬弓形蟲感染樣(yàng)品中檢出32份陽輛票性，并且和克隆測序結果一緻。結果表明，本研究建立的豬弓形討喝蟲實時(shí)熒光定量PCR檢測方法可用于豬弓形蟲的快速檢測，唱月從而爲豬弓形蟲病的診斷提供了特異、敏感、高吃麗通量的方法。Development and Applicatio新下n of a Real-time 女農PCR Method for Detection 人身of Toxoplasma gondii In order to e區站stablish a rapid，sensitive and specifi話金c method for detection of Tox見畫oplasmas gondii（T. gondi），a p湖金air of specific primers a門男nd FAM（fluorescein）-labeled站懂 MGB probes were desi票喝gned based on the conserved gene草年 sequences of T. gondii. Followed by op體去timizing PCR reaction system and co微議nditions，a real-time flu鐘廠orescent PCR assay was樂醫 established，and the spe了老cificity，sensitiv影通ity and reproducibility 計線test were carried out；then 60 clinica玩街l samples suspected of T. gondii 看少infection were tested by the e也兵stablished method. The results員你 showed that the method expres草人sed a good linear re哥如lationship when the template 鄉機was within the range of 101–107 議吃copies/μL. Specificity 影南test showed that posi謝水tive signal was observed o司外nly when amplifying t照資he recombinant plasmids of T. gon章近dii，rather than the water of nega個得tive contrast or other 7 pathogens；the 姐那minimum concentration of detect還劇ion template was 10店水 copies/μL；32 out of 60 suspected sa服呢mples were detected positive習一，and the result was consistent wi身木th that of cloning and sequencing. As書草 a conclusion，the established m學工ethod in this study市刀 could be used for rapid能湖 detection of T. gondii，which pro多區vided a specific，sensitive and hig話呢h-throughput method for t計時he identification of toxoplasmosis.全站說文下載鏈接：https://kns.cnki.net/KCMS/detail/老們detail.aspx?dbcode=CJFQ&dbname=CJF很對DAUTO&filename=ZGDW202005020&v=MTYwNz中火hyTVB5clBlYkc0SE5I懂票TXFvOUhaSVI4ZVgxTHV4WVM3RGgxVDNxVHJXTT是腦FGckNVUjdxZlllWnNGeXpoVXI=
2020-06-04
美洲型豬繁殖與呼吸綜合征病毒實時(shí)熒光R很風T-PCR定量檢測試劑盒測量不确定度研究
爲填補測量不确定度在獸醫領域病原學(xué)檢測的空白，建立影兵豬繁殖與呼吸綜合征病毒（PRRS紅門V）實時(shí)熒光定量RT-PCR檢測試劑盒測量不确定度評鐵火估模型，對(duì)市售的3種(zh中玩ǒng)美洲型PRRSV實時(shí)熒光RT-PCR檢測試靜離劑盒進(jìn)行了測量不确定度評估，評估如就了移液器、溫度、重複性測量因素對那器(duì)試驗結果産生的不确定可北度影響。研究數據表明：移液器與重複性測量對(duì)試驗結果産生的不确定度影響弟事較大，應予以重視，需規範操作；建立的3種(zhǒng)試劑盒測量不确定度模型縮湖鄉小了試劑盒對(duì)可疑樣(yàng)品的判定區域，對(duì)樣(yàng醫外)品臨界參考值的檢測結果判定具有實際意義。通過(guò)比對(duì)4個廠商內家的核酸提取試劑，篩選出了提取效率最高的試劑盒。本研究建河做立的PRRSV實時(shí)熒光RT-PCR物好定量檢測試劑盒測量不确定度模型爲獸醫實驗室檢測結果不确定度評估及應用奠定了基礎坐放。 Study on the Unce鐵畫rtainty of Real-time PCR Kits for Qua女站ntitative Detection of PRRSV of Am件算erican TypeIn order to fill in the bla冷木nk of the measurement uncertainty in th在懂e field of veterina件麗ry etiology，an evaluation m學但odel of measurement uncertainty for rea畫我l-time PCR kits f化件or quantitative detection of P好為RRSV was established，and three k鐘快inds of commercial風冷 RT-PCR kits（detecting PRRSV st月微rain of American t相你ype）were evaluated，including the influe報費nce of pipette，temperature and repeated輛街 measurement on te要麗st results. It was 秒短found that the test results were grea新冷tly influenced by the pipe分喝tte and repeated measurem議村ent，which should玩林 be paid attention to，and the operati短外ons should be standar秒冷dized；the determinatio視不n area of the kits for su中黑spected samples was reduc音厭ed，which contri南藍buted to the determination of th明秒e critical reference value of samp務開les. The kit wi笑通th highest extraction effici紅在ency was selected through co答畫mparing the nucleic a在湖cid extraction reagents 弟舊produced by four manufacturers. In conc地訊lusion，a foundation was hereby laid 腦也for evaluation and application of me子技asurement uncertainty in vete森近rinary laboratories by視光 the model esta議來blished in this study. 全文下載鏈接：h外睡ttps://kns.cnki.net/KC工劇MS/detail/detail.aspx?dbcode=CJFQ&dbn得銀ame=CJFDAUTO&fi謝知lename=ZGDW202005018&v=Mj開可I2NzZXcnpOUHlyUGViRzRITkhNcW85RWJJUjhlW化看DFMdXhZUzdEaDFUM3FUcldN道還MUZyQ1VSN3FmWWVac0Z5emc=
2020-06-04
基于CRISPR/Cas9技術快速構建來船PRV gE基因缺失病毒
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2020-06-04
A型塞内卡病毒研究進(jìn)展
A型塞内卡病毒（Senecavirus A，SVA）是新發(fā)現的一種(現場zhǒng)影響養豬業的RNA病毒河來，現已在美洲的美國(guó)、加拿大、巴西、計有哥倫比亞以及亞洲的中國(guó)、泰國(guó)、越南等爸老多個國(guó)家被(bèi)兒對發(fā)現。2015年SVA首次在我國(guó)廣東省發(煙公fā)現後(hòu)，已有15事靜個省、自治區和直轄市報道(dào)檢機務測到該病毒，表明SVA已在我國(guó)不同地域廣泛分布。豬感染SVA後行銀(hòu)的臨床症狀與口蹄疫、豬水泡會都病及水泡性口炎等類似，難以鑒别。SVA的實驗女街室診斷多采用病原學(xué)和血清學(xué)方法。對(d站兵uì)于該病目前仍無商品化疫苗可用說道，但已有研究出重組活疫苗候選株姐資和油佐劑滅活候選疫苗的報道(dào)。SVA未來的流行放但态勢目前難以預測，因此需密切關注SVA女水在全球的流行現狀，并深入研究SVA的緻病及免疫筆公機理，同時(shí)盡快研發(fā)有效的疫苗和診斷試劑如低。通過(guò)病原學(xué)、臨床症狀、流行狀況、診斷方法、疫苗研發(分美fā)等方面(miàn)的綜述，本文爲我國(guó)SVA防控提玩亮供了參考。Research Progress on 煙爸Senecavirus AAs an em物嗎erging RNA virus in pig industry，Senec年海avirus A（SVA）has been found in many 嗎對countries，including the United States自話，Canada，Brazil，Columbia，China，開電Thailand，Vietnam，etc. I機湖n China，the virus has been reporte放很d by 15 province海知s/autonomous regions/municipalitie近玩s since 2015 when it was 身著firstly found in Guangd嗎那ong，indicating 為樂a wide prevalence in dif習爸ferent regions. It was diffic年器ult to identify the clinical symptoms 件上of infected pigs th她新at were similar to those of foo上離t-and-mouth disease，swine vesicular 技術disease and vesicular謝呢 stomatitis，hence the metho厭放ds of etiology and biology 器煙were widely used by SVA detection 輛呢laboratories. No any冷時 corresponding commer兵年cial vaccines were available，but 下事recombinant live vacci醫月ne candidate strains and oil-ad可書juvan inactivated購暗 candidate vaccines had be腦懂en developed and r公在eported. Consider的站ing current difficu員鐘lty in prediction of 煙友the development of SVA，it was necessa不紅ry to pay attention to its global p少區revalence，and to 用年study its pathogenesis and immune mecha不知nism so as to develop ef房嗎fective vaccines and diagn山件ostic reagents soon他站est. In this paper，the etiolog著風y，clinical symptoms，ep學月idemic status，diagnostic輛音 methods，vaccine resear子報ch were discussed for the purpose of pr還姐oviding some references for th玩商e prevention and control of S市生VA in China. 全文下載鏈接：http行飛s://kns.cnki.ne花這t/KCMS/detail/detail.aspx?db會黃code=CJFQ&dbname=CJFDAUT用都O&filename=ZGDW聽答202005014&v=MjQwNDhZZVpzRnl6blc3N0tQeX美線JQZWJHNEhOSE1xbzlFWUlSO紅輛GVYMUx1eFlTN0RoMVQzcVRy街車V00xRnJDVVI3cWY=熱長
2020-06-04
非洲豬瘟病原學(xué)和流行病學(xué)研究新進(jìn)展
全球非洲豬瘟疫情形勢十分嚴峻，201外老9年以來共29個國(guó)家/地區報告了新發(fā)或正在流行的疫情，非洲話物豬瘟的防控備受全世界關注。爲全面(miàn)了解非洲豬瘟病毒（ASFV）爸為特性，概述了近年來對(duì)ASFV病原學(xué資東)特性和流行病學(xué)特點的一些新認識：ASFV對(duì)外界抵抗請不力較強，但怕幹燥、高溫、強酸和強堿；除家豬、野豬、軟蜱外，蒼蠅也可能(né醫城ng)扮演著(zhe)儲存宿主的角色；污染的飼料、飲喝黃用水也是ASFV的重要傳染源，其中飲水造成(chéng)ASFV感信務染的劑量更低，二者的風險點相差1萬倍，防控時(shí)要高度飛這重視飲用水；非洲豬瘟傳播途徑較多，但肉制品、環境、設備弟草或車輛、人員是傳播的重要風險因素，管理好(h爸近ǎo)其流動是防控的關鍵。本文對(duì)于識别非洲豬瘟的所有潛在傳染裡員源和傳播途徑，以便采取更有效的防控措施具有參考意義。 R到數esearch Progress鐘船 on Etiology and Epid國算emiology of African Swine FeverThe e火答merging or prevalent outbreaks o制城f African swine fever（ASF）老也have been reported少雨 in 29 countries/territ算道ories since 2019，it市影s prevention and contr要中ol has been concerne資動d around the world due to the seriou上務s threats. In order to make cl去化ear the characteristics of African能熱 swine fever virus（ASFV），some new know購銀ledge about the et房多iology and epid兒山emiology found in recent ye技些ars was summarized，that was，the virus w雪了as with strong resistance 的見to outside conditions，except drynes兵空s，high temperature，st姐見rong acid and st房金rong alkaline；its reservoir host電信s might include flies in 商錯addition to domestic pigs，wild b錢媽oars and soft ticks；the virus migh資嗎t spread via polluted 聽睡feed and drinking water，especi化書ally the latter that needed to 低習be highly concerned due to its 雪媽risk level which廠明 was 10 000 times higher than that 金銀of polluted feed；in spite 員愛of various transmis說草sion pathways，it was章鐵 the key to manage th算黑e movements of m家動eat products，equip妹技ment or vehicles and p舊車ersonnel as well as env山唱ironmental changes that w知話ere the major risk factors. As商頻 a conclusion，the study m的工ight contribute to the im司吃plementation of effective pr有兵evention and control mea去近sures through id上麗entifying all potential sou空男rces and transmission pathways o大請f ASFV.全文下載鏈接：https://kns.cnki.net/們自KCMS/detail/detail.議會aspx?dbcode=CJFQ海玩&dbname=CJFDAUTO&file樂家name=ZGDW202005013&v=MjY2MzBac0Z5em5弟器VTHZOUHlyUGViRzRITkhNcW85RVo0UjhlWDFMd看市XhZUzdEaDFUM3FUcldNMUZyQ1VSN3FmWWU=
2020-06-04
2014—2018年我國(guó)5株基因VII型新城疫病毒的基因組特近一性
爲了解我國(guó)基因VII型新城疫病毒的分子生物學(朋房xué)特性，對(duì)2014—2018年分離的5株基因VII型新城疫病動河毒代表株進(jìn)行了基因組測序和生物信息站快學(xué)分析。序列分析顯示：5株病毒F蛋白裂解位點爲112報請RRQKR/F117或112RRRKR/F117，具有強黃術毒株的典型特征；病毒基因組長(cháng)度均爲15 1家書92 nt，其F蛋白融合肽、七肽重複區和跨膜區，以及HN蛋白跨膜區、中和抗原草亮表位等功能(néng)區有多處氨基酸變異。病見樹毒F基因遺傳進(jìn)化分析顯示：201雜土4—2015年分離的2株病毒屬于基因VII拍技.1.1亞型；2016—2018年分離的3株病毒屬于基因VII.2亞型，不都暗示此亞型毒株可能(néng)已逐步取代基因VII.1.1亞機村型，成(chéng)爲家禽中的優勢流行毒株。本研究進(jìn)一步見門掌握了我國(guó)基因VII型新城疫病毒的遺傳特性，從而爲科學(樹舞xué)防控該病提供了參考。 Genomic C秒校haracteristics of Five Stra科船ins of Genotype VII Newcastle輛區 Disease Viruses Isolated 會信in China from 2014 to 2018線工In order to recognize the m見關olecular biological charac這看teristics of genotype VII Newcastle di高子sease virus（NDV）in China，five吃作 strains of representativ放作e genotype VII NDVs isolated from 2笑開014 to 2018 were sequen城煙ced and analyzed. 光年It was found that the c匠道leavage site of F pro山微tein of the five isolates was 1業內12RRQKR/F117 or 11我做2RRRKR/F117，with the typical char體土acteristics of virulent strain女花；the complete lengt船計h of virus genome was 15 192 nt，and se雪和veral amino acid mutation姐木s were identified in the fus一土ion peptide，heptad repeat regi是大on and transmembrane d影森omain of F protein，and the transm見如embrane domain and neutralizing e錯哥pitope of HN protein. Analys通裡is on genetic e長到volution of F genes sho話南wed that，the two strains 草多isolated from 2014 to 2015 belonge區那d to sub-genotype 讀和VII.1.1，and other three isolated f用坐rom 2016 to 2018 fe們刀ll into sub-genot拍新ype VII.2，which might gradually repl道個ace the sub-genotype V請子II.1.1 and become dominant in poultry少得. In short，the gene日了tic characteristics of NDV gen相錢otype VII were identified，which pr謝上ovided some refe妹會rences for scientific prevention and 離房control of Newcastle disease. 全爸服文下載鏈接：https://kns.cnki.net/KCMS/de術國tail/detail.aspx?dbcode=CJFQ&db錯科name=CJFDAUTO&filename=ZGDW20200你飛5008&v=MjQ3NjllWnNGeXpuVXJ6QVB5c音慢lBlYkc0SE5ITXFvOUZiSVI4ZVgxTHV4WVM3得制RGgxVDNxVHJXTTFGckNVUjdxZlk=
2020-06-04
我國(guó)台灣地區H5亞型禽流感病毒遺傳進(j厭煙ìn)化分析
2019年以來，我國(guó)台灣地區多次暴發(fā)H5人數亞型禽流感疫情，病原包括H5N2、H5N5、H7N7等子從亞型禽流感病毒。爲了解台灣地區流行的H5亞型禽流感病毒北算的分子流行特點，下載GenBank Influenza Virus Datab金器ase和GISAID中公布的台灣地區所有有志H5亞型禽流感病毒HA序列，對(du男男ì)近年來公開(kāi)報道(dào)的台灣地區流行的H5亞型禽流感病毒進(j道河ìn)行遺傳進(jìn)化分析，并與大陸地區流行的H5亞型禽遠照流感病毒進(jìn)行比較。結果顯示：台灣地區同時(shí)存在歐亞譜系文靜和美洲譜系兩(liǎng)個分支的H5亞型禽流感病毒流行，而大體樂陸地區流行的H5亞型病毒都(dōu)是歐亞譜系。民對歐亞譜系中，台灣地區流行的主要是第2.3.4.4a分支病毒，而大陸地區流行鄉窗的主要是2.3.4.4d分支病毒，兩(liǎng)訊道個地區流行的H5病毒譜系并不完全一緻。由于台灣地區的禽流感生态系物男統與大陸地區南方相似，因此台灣地區流行的美洲譜系H5亞和內型禽流感病毒有通過(guò)野鳥傳藍工播傳入大陸地區的潛在風險，應提高警惕，加強禽流感調查監測，嚴厲查處非法走私禽類腦那及其産品的活動。本研究爲我國(guó)H5亞型禽流感防控提供了信息支持。。 謝微Phylogenetic Analys不睡is on H5 Subtype Avia街光n Influenza Virus in T房技aiwan of ChinaH5 subtype avian in離一fluenza（AI）including H5N2，H5N5水刀 and H7N7 had tak會數en place several times in Tai土街wan of China since 20用視19. In order to identify the 還匠molecular characteristics of H5 subt匠費ype AIV prevalent頻水 in Taiwan，all HA sequences o花我f the viruses isolated from Ta煙明iwan registered in人人 GenBank Influenza Virus Resource a工訊nd GISAID Database were那玩 downloaded to analyze their evolutio的歌nary relationships，是大and these H5 subtype AIV strai窗動ns were compared with those prevalent i機刀n mainland China.區雪 The results showed that the H5 subt開業ype AIV prevalent in Taiwan belonge中可d to Eurasian or American linea民嗎ge，while the strains prevalent in m內電ainland China only fe器數ll into Eurasian li我微neage. For Eurasian line報工age，clade 2.3.4吃遠.4a virus was mainly prev草哥alent in Taiwan，while cl就能ade 2.3.4.4d virus was prevale子爸nt in mainland China.車厭 As avian influ妹農enza ecosystem in Taiwan was si習生milar to that in sout術低hern mainland Ch時男ina，the viruses of American個頻 lineage prevalent in Taiwan h花票ad a potential risk of b讀火eing introduced 商站into mainland China via wild birds，微的so it was necess下拍ary to improve early warning system，做那strengthen investig學湖ation and surveillance on AIV，inves黑海tigate and handle with an公老y illegal smuggling 身藍of poultry and related弟都 products. In c兵她onclusion，some data was prov店又ided to prevent and contro筆一l H5 subtype AI in China對不..全文下載鏈接：https://kns.cnki.n新影et/KCMS/detail/detail.asp雪市x?dbcode=CJFQ&dbname=CJFDAUTO&filename=術科ZGDW202005007&v=MTA5MTR6bVZMM09Q多白eXJQZWJHNEhOSE1xbzlGWTRSOGVYMUx1筆長eFlTN0RoMVQzcVRyV對作00xRnJDVVI3cWZZZVpzRnk=
2020-06-04
2019年我國(guó)部分地區屠宰生豬旋毛蟲和中制弓形蟲檢測
爲了解我國(guó)部分地區屠宰生豬數一旋毛蟲和弓形蟲感染情況，2019年在廣東（5個）花校、山東（4個）、雲南（6個）等省份的15個大型、中小型生豬去刀屠宰場，采集豬膈肌樣(yàng)品315份，用PCR方法進(jìn)從老行旋毛蟲和弓形蟲病原學(xué)檢測；在以上3省15哥現個大型、中小型生豬屠宰場（每省5個），采討美集血清樣(yàng)品254份，用ELI算醫SA方法進(jìn)行旋毛蟲和弓形蟲血清學(x懂道ué)檢測。結果顯示：經(jī時一ng)PCR檢測，采樣(yàng)地大算區所有膈肌樣(yàng)品均爲旋毛蟲陰性，僅在雲南省120農也份膈肌樣(yàng)品中檢出1份弓形蟲陽性；經(jīng)ELISA檢測土多，采樣(yàng)地區血清樣(yàng)快看品的旋毛蟲和弓形蟲抗體陽性率分别爲1.97%和2.一熱36%，不同省份的陽性率分布不一緻刀知，中小型屠宰場陽性率均高于大型屠宰場。結果表明，廣東看志、山東、雲南等省份屠宰生豬的旋毛蟲和弓形蟲攜帶率極低，基本可以保證豬舞下肉的産品安全；部分地區屠宰生豬存在一定的弓形蟲感染抗體，尤其是中小型屠宰服紅場，表明此類豬群需要加強飼養環節的房呢弓形蟲感染控制。本研究爲保障豬肉食品安全及市事飼養環節的豬旋毛蟲和弓形蟲感染控女民制提供了依據和指導。 Detection of Trich下年inella spiralis and Toxopl可們asma gondii in Pigs for 子火Slaughtering in Some Regions of China費信 in 2019 In order to make clear 舞廠the prevalence of Trichinella spiral亮和is（T. spiralis）an樹見d Toxoplasma gond畫街ii（T. gondii）in pigs for 喝拍slaughtering in some re北我gions of China，in 201事計9，315 diaphragmatic samples were coll那家ected from 15 large or small 子坐and medium slaughterhouses i人下n Yunan（5 farms），Shandong（4 廠爸farms）and Guangdong（5 farms）森冷provinces for etiological de如服tection by PCR；and 254 serum s兵影amples were collected from the slau拿秒ghterhouses in the above three prov鐘弟inces（5 slaughte鐘志rhouses per province）for s靜農erological test by ELISA. T飛喝he results showed tha文嗎t，for PCR detection，all the diaph理從ragmatic samples were nega亮還tive for T. spiralis，and only o弟書ne out of 120 samples from Yunnan wa兒裡s positive for T. gondii；f金商or ELISA detection，the positi長愛ve rates of antibod工爸ies against T. spi年少ralis and T. gondii雜算 were 1.97% and 2.36%，respec這了tively，with different distribution 我要in different province地森s，that was，the posi商吃tive rate of small and medium sla業訊ughterhouses was higher than校小 that of large ones. In sh做爸ort，the carrying窗好 rate of T. spiralis and T. gondi用呢i in the pigs for slaughteri老友ng in the above provinces wa玩腦s extremely low，which coul一飛d ensure the safety of pork produ鐘冷cts；infection of T. gondi新關i were presented in some regions in cer生家tain degree，espe人靜cially in small and medium slaughte空玩rhouses，indicating 畫在that T. gondii should 你議be controlled in the feeding proces遠醫s of such pig population. Some 花鐵basis and guidance wer姐銀e hereby provided to safeg他做uard the safety of pig 購雪products and to control infect紙放ion of T. spiralis a在輛nd T. gondii in 技話feeding process.全文下載鏈接：https://kns.湖吧cnki.net/KCMS/detail/detail.aspx?dbc時亮ode=CJFQ&dbname=CJFDAUTO&音間filename=ZGDW202005001&v=MDMxMjh從城ITkhNcW85RlpZUjhlWDFMdXhZU還你zdEaDFUM3FUcldNMUZyQ1VSN3F黃花mWWVac0Z5am5WTDNKUHlyUGViRzQ可又=
2020-06-04
志賀氏菌檢測和分群實時(shí)熒光PCR方法的建立
爲對(duì)志賀氏菌屬細菌進(j亮件ìn)行快速、準确檢測和分群鑒定，根據志賀雨商氏菌invC、rfc、wbgZ和rfpB基因，分别設計引物光人和探針，建立了鑒定志賀氏菌屬以及福氏志賀氏菌、宋内多愛志賀氏菌和痢疾志賀菌氏菌實時(shí)熒光P拍跳CR方法，同時(shí)將(jiāng)根據ompA基因設紙謝計的引物和探針作爲内參照，并通過(guò)特異匠都性試驗和人工接種(zhǒng)試驗等對(學鐵duì)該方法進(jìn)行驗證。結果顯示，該好什方法對(duì) 17 株志賀氏菌和 19株非志賀氏菌均出現100%的特們長異性；用增菌肉湯直接進(jìn)行PCR檢測，發(fā)現在小舞消毒奶、冰淇淋、酸奶、奶酪、熟肉和香和服腸等即食食品中的志賀氏菌檢出限爲1~3 cfu/25 g（mL），在原奶、要鐘生肉和奶粉中的檢出限分别爲≤8 cfu/25影訊 mL、12 cfu/25 g和≤12 cfu/25 g子從；分離培養後(hòu)再對(duì)可疑菌落進(jì信問n)行實時(shí)熒光PCR鑒定，發(fā)現所暗理有樣(yàng)品的檢出限爲1~3 cfu/25 g（mL），與傳統培養法他放檢出限一緻。結果表明，該實時(shí)熒光PCR方法是一個快速、敏感、特異的檢的商測方法，适合于志賀氏菌的常規檢測分析。Estab但是lishment of a R很我eal-time PCR Assay for 兒就Detecting and Classifying Shigell煙畫aIn order to detect and cla件到ssify Shigella bac紙綠teria rapidly and accurately，the火厭 primers and probes were地裡 respectively desi熱妹gned according to the genes計道 of invC，rfc，wbgZ and rfp煙店B，and a real-time PCR ass們我ay was established to identify S玩商h. castellani including Sh. flexneri，些些Sh. sonnei and Sh. dys化子enteriae，then it was verifie吃水d by specific test and artifici光為al inoculation test w討謝ith reference to the 作子results of using the primers and p道算robes designed according to ompA讀也 gene. The results showed that th術愛e specificity was 100% when all 17 s月要trains of Shigella and 19 non-s能工higella strains we公明re amplified by the established assay；t在行hrough PCR assay with enrich冷腦ment broth，the dete房村ction limits of Shigella in pa要東steurized milk，ice cream，acidop雪明hilus milk，cheese，co但身oked meat，sausag校書e and other inst綠什ant foods were 火動1–3 cfu/25 g（mL），an鐘體d that in raw milk，raw meat影妹 and milk powder were≤8 cfu/25 通船mL，12 cfu/25g and≤12 cfu/25 g，respec習他tively；the suspicious bacterial col門子onies were isolated 用些and cultivated for行村 real-time PCR assay，it was fou熱麗nd that the detection lim在離it of all samples w錯慢as 1–3 cfu/25 g（mL），which was cons美道istent with the result by trad子高itional cultivation method. In要鐘 short，the real-time PC愛得R assay was rapid，sensitive a上技nd specific，whic問家h was appropriate for r房錢outine detection an地門d identification of Shigella.全文下載鏈接：ht嗎科tps://kns.cnki.net/KCMS/detail/de雜志tail.aspx?dbcode=CJFQ&dbname=CJFDAUTO&秒時filename=ZGDW202003023&v知會=MjUxNzhNMUZyQ1VSN3FmWk9k小木dEZ5am1WN3ZJUHlyUGVi藍不RzRITkhNckk5SFo0Uj分行hlWDFMdXhZUzdEa長鐘DFUM3FUclc=
2020-03-12
雞傳染性喉氣管炎病毒實時(shí)熒光PCR檢測方法的建立與應用
爲探索适合臨床高通量雞傳染性喉氣管炎檢測的方法，針對(duì)雞傳染性喉氣讀員管炎病毒gB基因序列保守區域，篩選空金1對(duì)特異性引物和1條特異性探針，建立了會腦實時(shí)熒光PCR檢測方法，并對到章(duì)該方法進(jìn)行特異性、敏感性評估和臨床應用檢測。結果顯示：篩選上南出的引物和探針與其他禽類常見病毒無交叉反應，檢測下限達到新樹100拷貝/反應；對(duì)來自13個場的480份臨床樣(yàng)本進(j街著ìn)行檢測，檢測出17個陽性樣(yà員習ng)品，與常規PCR檢測結果符合率爲100%。結果鐘站表明，此方法特異性強，靈敏度高、耗時(shí)但內少，可用于雞傳染性喉氣管炎的快速檢測。Establishm讀海ent and Application 志間of a Real-time PCR fo跳票r Detecting Infe紙木ctious Laryngot知話racheitis VirusIn order to develop an學去 appropriate method for high-thr木地oughput detection of avian infec但間tious laryngotracheitis（AILT），a re很好al-time PCR assay was established wi舊冷th a pair of specific primers子高 and a probe ba老著sed on the conservativ公書e zone of gB gene sequence of AILT，為道then its specificity and sensitivity 城空were evaluated，and its clini厭志cal application was tested. 裡店The results showed that the sel匠森ected primers and probe faile道車d to cross-react票算 with other common poultry viruses，wit森街h the detection limit of 100 copies/能子reaction. 17 out of 480 clinical作農 samples collected from 13 farm冷商s were positive.低我 The results completely conformed to th風不e test result by下離 common PCR assay. As a conclusi是長on，AILT could be rapidly detecte村時d by the established met去黃hod with high spe務數cificity，high s道東ensitivity and less 鐘能time-consuming.全文下載鏈接：https:/都業/kns.cnki.net/KCMS/detail/det弟爸ail.aspx?dbcode=CJFQ&dbnam冷個e=CJFDAUTO&filenam來遠e=ZGDW202003021&v=MTM1MDBQeXJQZWJHNEhOS子下E1ySTlIWllSOGVYMUx1eFlTN0RoMVQzcV現著RyV00xRnJDVVI3cWZaT2R0RnlqbVU業路3N04=
2020-03-12
禽源HSP70、HSP40和RPL4基因的克隆和表達
本研究參照GenBank中編碼禽源熱休克蛋白HSP70、HSP40媽書 和核糖體蛋白RL4等的基因序列志吧，分别設計特異性引物，采用RT-PCR擴增HSP還購70、HSP40和RPL4基因，經(jīng)雙酶切後內什(hòu)克隆到真核表達載體pEF1α-Myc，得銀呢到重組質粒pEF1α-Myc-H我術SP70、pEF1α-Myc-HSP40和pEF1α-My舞放c-RPL4；將(jiāng)構建好(hǎo)的重組質粒，轉醫下染HEK293T細胞後(hòu)，體間采用間接免疫熒光和Western-blot技術，對(duì)目的蛋白進近道(jìn)行驗證。結果表明，Myc-HSP70、Myc-HSP40和Myc船嗎-RPL4融合蛋白在HEK293T細胞内得到了正确表達。多水本研究爲後(hòu)續禽源HSP70、HSP40和RPL4基因人嗎的功能(néng)研究奠定了基礎。Cloning and Expre購讀ssion of Avian-origin 南媽Genes of HSP70，HSP40 and RPL4In this她雨 study，specific primers were 們道respectively designe在章d according to the gene sequences 業人of the heat-shock 唱好protein（HSP70 and HSP4離友0）and the ribosomal protein（RPL4）regi裡綠stered in GenBank，then the genes of得鐵 HSP70，HSP40 and R請短PL4 were amplified by RT-PCR，and af工能ter double enzyme digestion，the qualifi體近ed genes were cloned into the e微暗ukaryotic expression plasmid，物議pEF1α-Myc，to obtain the recombinant pla畫動smids including pEF1α-Myc-購機HSP70，pEF1α-Myc-HSP40 and pEF1α-Myc-R門年PL4；after the recombinant plasmids南校 were transfected into HEK293T ce器答lls，the expressed protein was verified 作熱by indirect immunofluorescence and 請遠Western-blot. Th人體e results showed that the rec風都ombinant proteins of Myc-HSP70，Myc朋技-HSP40 and Myc-RPL4 were correctl河書y expressed in HEK293T cells. Therefore地電，the foundation was laid for fur師用ther study on the genes of HSP西但70，HSP40 and RPL4.全文下載鏈接作體：https://kns.cnki.net務東/KCMS/detail/detail.aspx?dbcode=C技文JFQ&dbname=CJFDAUTO&filename=關分ZGDW202003020&v唱體=MDI5OTRqbFdyN0xQeXJQZWJHNEh弟筆OSE1ySTlIWklSOGVYMUx1eFlTN0RoMVQzcVR煙在yV00xRnJDVVI3cWZaT2R0Rnk=
2020-03-12
山東省3家鹦鹉養殖場多瘤病和喙羽病的病原檢測與序列分析
2018年山東省某養殖場發(fā)生大量幼齡鹦鹉死亡事(shì)件，疑似還林爲病毒感染。爲探尋病因，開(kā她近i)展了該場及省内另外兩(liǎng)個鹦鹉相校養殖場的流行病學(xué)調查。利用P家如CR技術對(duì)臨床樣(y水麗àng)品中提取的DNA或RNA進(jìn)行檢測，結果發(fā)現新城疫與禽鐘光流感病毒均呈陰性，而禽多瘤病毒（APV1）與鹦鹉喙新兵羽病病毒（PBFDV）呈現爲強陽性，由此推斷此3個鹦鹉養車城殖場存在APV1和PBFDV感染，平均病毒檢出率分别爲67業拿.9%與71.4%，共感染檢出率率爲國站58.0%。對(duì)陽性樣(yàng)品進(jìn)化全基因分析發老北(fā)現：區域内的PBFDV流行毒株同源性高，與該地區去腦早期報道(dào)的毒株親緣關系較爲接近；A機身PV1的VP1基因同源性較高，與歐洲分離毒株親緣關系較爲接近土麗，說(shuō)明我國(guó)流行的APV1或來源于進(j懂玩ìn)口鹦鹉。本研究警示，需要加強鹦鹉疾病防控，嚴格鹦鹉進(jìn)出口檢林綠疫，并制定和健全标準的鹦鹉病檢疫程序。Pathog動音en Detection and Seque事懂nce Analysis of Aves Polyomavirus 說飛1 and Psittacine Beak & Feathe能雪r Disease Virus in煙票 Three Parrot Far事月ms in Shandong ProvinceIn拍大 2018，a large num計術ber of young parrots die飛但d in a farm in Shandong P村月rovince，which was suspected of 技了being infected with virus. In order to喝答 find out relevant causes，an epidemiol們務ogical investigat關又ion was carried out in the infe機現cted farm and other 你影two farms in Shan那拿dong Province. DNA 你又or RNA extracted from clinical司學 samples was tested by PCR，it was 從媽found that Newcastle disease virus（NDV什如）and avian influenza virus（AIV）we唱哥re detected negative，while Av笑人es polyomavirus 1（APV1）and p學短sittacine beak & feather disease vir他計us（PBFDV）were detected strongly pos去暗itive，therefore，a 行是conclusion was given that parrots 聽請in the three farms had alread男線y been infected with APV1 a司請nd PBFDV，with average positive ra雪老te of 67.9% and 71.4% respective學山ly，and the average co-infection 城和rate was 58.0%. As per th舊外e results of whole-genome analys了睡is on these positive samples，th數煙e prevalent strains of PB多刀FDV in the region s件好hared high homology雪遠，which was more similar to those report一農ed earlier；the VP1 genes of A員的PV1 were with higher homology，which wer高坐e more closer to thos厭業e isolated in Europe，i友腦ndicating that the AP會嗎V1 prevalent in 在黃China might be in船煙troduced by impo長腦rted parrots. Hence，it was n麗木ecessary to strengthen the preventio木做n of parrot diseases a紅服nd increase quarantine都綠 indicators related to i湖哥mported parrots in Chi熱信na.全文下載鏈接：https議紅://kns.cnki.net/KCMS/deta從筆il/detail.aspx?dbcod影月e=CJFQ&dbname=CJFDAUTO&f低鄉ilename=ZGDW202003022&門資v=MTAxMjJDVVI3cWZaT2R0Rn農西lqbVVidk5QeXJQZWJHNEhOSE1yS森姐TlIWm9SOGVYMUx1eFl吧上TN0RoMVQzcVRyV00xRnI=
2020-03-12
結核病實驗室檢測技術研究進(jìn)展
結核病（tuberculosis，TB）是由結核分枝杆菌（Mycobacter計市ium tuberculosis，MTB）引起(qǐ)的一種(zhǒng)個們人獸共患慢性傳染病，其早期診斷和檢測非常困難。常規的TB實驗室檢測技術主要是痰志城塗片和痰培養，這(zhè)是診斷肺結核的金标準，但均有一定的局限性用近，故對(duì)其優化和改良是目前TB檢測研究的一個重要就少方向(xiàng)，目前主要集中在硬件設備的升級改造上。近年來，TB實驗檢錯動測診斷技術發(fā)展較快。核酸擴增方法成(chéng)爲白水TB診斷研究的新方向(xiàng)，包括環介導等溫擴增暗微（loop-mediated isothermal amp飛子lification method，LAMP），結核分枝低也杆菌/利福平耐藥實時(shí)熒光定量核酸擴增河場（Xpert Mycobacte-r下器ium tuberculosis/Rifamfampin，Xper腦員t MTB/RIF），交叉引物恒溫擴增（crossing pri睡腦ming amplificatio靜是n，CPA），實時(shí)熒光恒溫擴增（simultan北光eoous amplification a西章nd testing method，SAT）以及PCR技月爸術等。免疫學(xué)檢測技術是TB檢測的另一個重要發(內火fā)展方向(xiàng)，現已研視兵制出兩(liǎng)種(zhǒng)快速檢測方法T-S女雪POT.TB和QuantiFERON-Gold test（QFT）。區什還(hái)有一些新技術正在研發(fā)中，如基質輔助激光解吸電人熱離飛行時(shí)間質譜（matrix-assisted laser des謝器orption/ionization time of flight但街 mass spectrometry，MALDI-TOF-MS）以及代謝錢事标記檢測、納米孔測序等。TB檢測手段正由傳統的細菌學(xué)檢查轉向(x北讀iàng)免疫、分子診斷等更爲精确的方法。文金然而，不同診斷方法均有局限性，在風金實際應用中需采用多種(zhǒng)檢測方法相結合的方式。本文對(duì)于全面藍喝(miàn)了解多種(zhǒng)TB檢測技術的優點和局限性，從而就醫選擇合适的檢測方法具有幫助意義。Re些電search Progress on Lab體下oratory Technologies for Detection of T到亮uberculosisTuberculosis說志（TB）is a chronic zoonosis caus公謝ed by mycobacterium tub家紙erculosis（MTB），which is信什 very difficult to我小 diagnose and detect at its花科 early stage. Fortunately，the tec很飛hnologies of TB laboratory test鄉林ing and diagnosis has 就化been developing rapidly in recent 美醫years. As the main唱在 conventional testing 但城technologies，smear and cultiva頻資tion of sputum are gold st劇村andards，but both of the two tech如女nologies have some limitations，he愛車nce，their optimization and impr服冷ovement have become the impor我學tant direction for TB testing，wit技友h an emphasis on upgrading 樹也of related hardware equipm綠要ent. In order to solve such limitatio小物ns，nucleic acid amplif讀是ication methods have 答通been studied and explored，inc文雨luding Loop-mediated Isothermal Ampli北分fication（LAMP），Xp綠舊ert Mycobacterium tuberculosis/Rif銀很ampin（Xpert MTB/RIF），crossin慢會g priming amplification（CPA），simultan嗎下eous amplification and testing（SAT）影又and PCR. Immunological assays are als愛月o used for TB testing，in which，T-S個小POT.TB and QuantiFER農煙ON-Gold test（QFT）have been al日道ready developed. Other坐暗 new technologie動話s are also in developm商歌ent，such as Matrix-assisted會區 laser desorption ionizat時件ion time-of-flight mass spec上他trometry（MALDI-TOF器哥-MS），metabolic mark化文er testing，nano議笑pore sequencing，etc. At presen低月t，TB testing methods are tu信化rning to immunoassay，molecular diag問美nosis and other more accurate me短機thods from traditional bact美很eriological testing. H什討owever，all the above methods ar是區e limited，therefore differen機站t technologies should be used togeth能北er in practice. I銀體n conclusion，the advantages and l照湖imitations of various TB te會秒sting technologies were summariz資門ed，which would help to select ap冷友propriate method i多綠n practice.全文下載鏈接：https://kns.空但cnki.net/KCMS/de電樹tail/detail.aspx?dbcode=CJFQ&dbname=CJ是工FDAUTO&filename=ZGDW202003018&v=MD章些c1MjV4WVM3RGgxV就話DNxVHJXTTFGckNVUjdxZlpPZHRGeWpsVn和議J2QlB5clBlYkc0SE5ITX兵工JJOUViSVI4ZVgxTHU=
2020-03-12
冷凍原腸中細菌基因組DNA提取方法的建立
爲建立冷凍原腸中細菌基因組DNA的直接提空視取法，采用震蕩洗脫、金屬網過(guò)濾和梯度離心相結合的方法，對(廠光duì)2份冷凍原腸樣(yàng)品進(jì森懂n)行前處理；對(duì)經(jīng)前處理後(hòu西店)的原腸樣(yàng)品，采用酚制文/氯仿法提取細菌基因組DNA，然後(hòu)進(jìn)行瓊脂糖電泳和OD2工花60/OD280比值測定；以細菌基因組DNA爲模闆，用PCR技術擴增細菌16喝開S rDNA并構建克隆文庫，并從2個文庫中各随機商機挑取3個菌落進(jìn)行16S rDNA 的PCR鑒定和DNA測序筆讀。結果顯示：樣(yàng)品提取的DNA濃度、純度較高，OD260/OD280但店比值介于1.8~2.0；挑取的6個菌落中，1個爲陰性，剩餘5個爲陽性街快，爲腸球菌屬（Enterococ都文cus）和魏斯氏菌屬（Weissella）的5種(zhǒng)細菌。結果表明，信山本研究提取的原腸細菌基因組DN農間A質量較好(hǎo)，可用于非培養法研究冷凍原南日腸中細菌的多樣(yàng)性。本研究解決了因缺乏原腸細菌基因組DNA提取間黃方法，無法進(jìn)一步應用現代分子生物學(xué)方法研究冷凍原腸細土些菌的多樣(yàng)性、菌群組成子為(chéng)結構和動态變化等難題。Es得從tablishment of a Method for Extracting不吧 Bacterial Genome DNA from Frozen Arch數那enterons In order to establish a direct還裡 method for extractin愛些g bacterial gen筆來ome DNA from frozen arc船聽henterons，two samples of frozen archen暗小terons were pretreated by combination o北體f shake-elution，fi有開ltration via metal mesh and gra話就dient centrifugation te店不chnique；then the bacter鐘現ial genome DNA was extrac員議ted by use of pheno術很l-chloroform for agarose gel electr了家ophoresis and measurement o弟她f OD260/OD280 ratio；taking th和員e bacterial genome DNA as the template，問到16S rDNA was amplified by PCR ass北爸ay，then the clone libraries were公習 constructed. Three bacterial coloni說著es were randomly selected from each of答醫 the two libraries f見車or PCR identification an錯對d DNA sequencing o公姐f 16S rDNA. The results sh要上owed that the concentration an就物d purity of DNA extract遠不ed from the samples were rel什空atively high，and空著 the OD260/OD280 rat花司io ranged from 1.8 to 2.0；one out of 他子six selected bacterial colonies到家 was negative，and others wer聽小e positive，furthermore，t校兒he five species of ba飛呢cteria belonged to En問兒terococcus and Weissella. In c站也onclusion，the bacterial genome DNA extr路物acted from frozen森嗎 archenterons was of good qu訊照ality，which could be used to study th訊校e diversity of bacteria in f文能rozen archenterons by culture-高問independent method. With the met站視hod established草道 in this study，it 通輛was possible to co兒市nduct further study 開近on bacterial divers答國ity，community structure and variat樂拿ion by using moder子銀n molecular biolog能中y technologies.全文下載鏈接：https://河亮kns.cnki.net/KC快農MS/detail/detail.aspx?dbcode=道愛CJFQ&dbname=CJFDAUTO&filename=ZGDW不樂202002020&v=MjY5MzJGeW5廠銀oVkw3T1B5clBlYkc0SE5ITXJZOUhaSVI4ZVgxTH上樹V4WVM3RGgxVDNxVHJXTTFGckNVUjdxZl匠嗎pPZHQ=
2020-03-12
我國(guó)牦牛源冠狀病毒流行現狀分析
爲了解我國(guó)牦源牛冠狀病毒（BCoV）流行現狀，通過(g都相uò)查閱文獻資料方法，彙總分析我國(guó)牦牛主産地區的BCo理著V檢測情況。資料顯示：2012年以來，我道南國(guó)青海、四川、新疆、西藏等牦牛主産省（自治區）的牦牛群中普遍存木綠在BCoV感染且感染率較高，平均血清抗體陽性率多在80%以上，病原學(xué)音下陽性率多在60%以上。需采取加強飼養管理、控制混合感染、做好(hǎo)隔離姐河淨化等措施，控制牦牛群中的BCoV流行。我國(guó)的BCo什唱V研究起(qǐ)步較晚，流行病學(xu拍舊é)方面(miàn)的報道(dào)較少，有必要訊就進(jìn)一步加強該病毒在牦牛中緻病機理、流行情況和防治技術等方面頻費(miàn)的調查研究。本文爲掌握我國(guó)牦牛中月身的BCoV流行情況及其防控提供了參考。A體國nalysis on the Preval放說ence Status of Yak-derived Bovine Coro麗站navirus in ChinaI腦拍n order to investigate the pr吧微evalence status o風坐f yak-derived bovine coronavirus（BC但報oV） in China，relevant te綠森sting results in main product身人ion regions of yak were s票拍ummarized and analyzed by means市去 of reviewing liter工兒ature. As it was found tha金對t，since 2012，BCoV had bee黃分n widely prevalent in the main provi照兵nces（autonomous reg風行ions），including Qinghai，見舊Sichuan，Xinjiang and Tibet，with a h會謝igh infection rate，and th飛海e average positive rates計頻 of serum antibodies in most regions 妹銀were above 80%，the positive ra請音tes of pathogen日鄉s were above 60%. In orde姐厭r to control the di上小sease，a series of m妹腦easures should be carried少裡 out，such as，strengt知購hening husbandry management，prevent低件ing any mixed infect光不ion，and striving又不 to isolate and purify t民信he virus. However，there were few我就 epidemiological reports due to late要森 beginning of BCoV study in China，it wa吃自s necessary to further stre就他ngthen relevant investigation and resea物鐵rch on the pathogenic船討 mechanism，prevalence and control te見呢chnology of the disease分林. This study would provide服靜 some references for 時林identification of B公的CoV prevalence 腦土and its prevention and control in Ch樂物ina.全文下載鏈接：https://外影kns.cnki.net/KCMS/detail/detail.a數我spx?dbcode=CJFQ&dbname=我慢CJFDAUTO&filename=ZGDW202003003風學&v=MzA0NDZZUzdEaDFUM3FUcldNMUZy美短Q1VSN3FmWk9kdEZ5amtXN3pBUHlyUGVi樹票RzRITkhNckk5Rlo0U少視jhlWDFMdXg=
2020-03-12
新型冠狀病毒2019-nCoV與動物冠狀病毒進(jìn)化農跳關系分析
新型冠狀病毒（2019-nCoV）感染肺炎疫情自2不好019年12月在湖北省武漢市發請書(fā)生以來，已在我國(guó火森)和世界多個國(guó)家傳播，引發(fā)全球樂呢關注。爲闡明2019-nCoV與已知動物冠狀有林病毒的關系，從冠狀病毒的宿主分布、裡輛遺傳進(jìn)化分析、宿主受體分析等角度，對(妹我duì)2019-nCoV的可水也能(néng)來源進(jìn)行了綜合分析。結果顯示：2019-nCo光相V與從雲南省中菊頭蝠中檢測到的冠狀病毒分離株RaTG13基因組同源性最高，去路爲96.2%，兩(liǎng)者遺傳進(jìn)化關系也討制較爲接近；從我國(guó)廣東省和廣西壯族自治區窗鐵查獲的走私穿山甲中檢測到的冠狀病毒則與2019-nCoV遺傳關系相對匠什(duì)稍遠，與2019-nCoV基因組同源性分别爲90.4%和85藍短.5%；2019-nCoV與已知家畜、家禽，以及犬數好貓等寵物中檢測到的冠狀病毒基因組同源性≤54.2%，遺傳進(jìn)化關系較遠關通。結合監測結果，基本排除2019-nCoV來源于已知家畜、家禽以及犬金要貓等寵物冠狀病毒的可能(nén站關g)性。Phylogenetic Analysis betw商就een the Novel Coronavirus（2019信匠-nCoV）and Animal C謝土oronavirusesSince the outbreak of pn放跳eumonia caused by the nov窗信el coronavirus（2019-n水錯CoV）in Wuhan City o亮船f Hubei Province in Decem來暗ber 2019，it has spread i低愛n China and many other countries aroun月購d the world，which is the 美西focus of global atten購嗎tion. In order to clarify the r新腦elationship between 2019-nCoV an計熱d the known animal coronaviru樂秒ses，the comprehensive anal妹讀ysis was performe慢說d to find the possib多民le source of 2019-nCoV based on the線哥 host distribution，genetic phy小她logenetic analysis，host recepto線雨r analysis of coronaviruses拍跳 in the study. T學煙he results showed that the geno空也me homology between 2019-你低nCoV and the coronavirus Ra對自TG13 detected from Rhinolop車視hus affinis in Yun房路an Province was the highes學黃t（96.2%），and 2019-nCoV was close錢輛ly related to RaTG13 in genetic p子報hylogenetic analysi著內s. The genetic relatio照一nship between 2019-n山事CoV and the two coronaviruses de年窗tected from seized 風時smuggling pangolin in Guangdon睡什g and Guangxi were relatively long煙為er compared with RaTG13，and the genome明商 homology of the 2019-nCoV w著聽ith coronaviruses detected fr是河om seized smuggling pangolin 到樂in Guangdong and Gu我熱angxi was 90.4% and 85.5%小公，respectively. Genetic distance betw為路een 2019-nCoV and coronaviruses obtaine能時d from known domestic livestoc外時k，poultry，pets includin月友g dogs and cats was筆店 genetically long，and the genome 西綠homology of 2019-關作nCoV with them was≤54.2%. Combined w就知ith the published surveillance月麗 results，the possibilit自林y of 2019-ncov originating from the kn物銀own coronaviruses of domestic 刀到livestock，poultry，pets including草制 dogs and cats 從工is basically excluded. 全文下載鏈接：https:/文為/kns.cnki.net/KCMS/detail/deta熱窗il.aspx?dbcode=CJFQ&dbname=CJFDAU票子TO&filename=ZGDW202003002&v=MjI3MTVU說錢cldNMUZyQ1VSN3FmWk9kdEZ5amtWTC9QUH子劇lyUGViRzRITkhNckk5RlpvUjhlW什少DFMdXhZUzdEaDFUM3E=
2020-03-12
農業農村部派工作組赴玉樹州指導抗災保畜
依法做好(hǎo)非洲豬瘟防控工作——農業農村部畜牧獸醫局負責人就(jiù)非見靜洲豬瘟防控答記者問
市場監管總局、農業農村部要求豬肉制品生産企業進(jìn)月答一步做好(hǎo)非洲豬瘟防控
農業農村部部署2019年畜牧獸醫重點工作
中心組召開(kāi)學(xué)習（擴大冷姐）會(huì)議傳達學(xué)習“不忘就們初心、牢記使命”主題教育工作會(h電日uì)議精神
韓長(cháng)賦在全國(guó)非洲豬瘟防控工作視頻會(huì)上強國錢調 确保打好(hǎo)打赢非洲豬瘟防控攻堅戰 努力保障生豬産業持續市微健康發(fā)展
2018年我國(guó)農産品市場我花整體供應較爲充裕
澳大利亞默多克大學(xué)代表團訪技火問中心
農業農村部關于印發(fā)《2019年獸藥質量監督抽檢和風險監測計劃》的通知體好
淋巴細胞脈絡叢腦膜炎病毒實時(shí)熒光RPA快速檢測方法的建分機立
